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Bisulfite Treatment of DNA Anderson Lab - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Bisulfite Treatment of DNA Anderson Lab. Adapted from Frommer et.al. - [Read Bisulfite Treatment of DNA Anderson Lab]

Bisulfite Treatment of DNA for Methylation Analysis - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Adapted from Frommer et.al. Good protocol for bisulfite treatment of DNA. Includes tips on Methylation PCR for CpG methylation analysis. University of Texas M. D. Anderson Cancer Center - [Read Bisulfite Treatment of DNA for Methylation Analysis]

Bisulfite-PCR for Restriction Analysis and/or Sequencing - http://www.mdanderson.org/departments/methylation/display.cfm?id=8E0AAAAC-FA42-486C-A93F60CD6E1C7E51&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Bisulfite-PCR for Restriction Analysis and/or Sequencing. Protocol written by Jean-Pierre Issa. M.D Anderson Cancer Centre Texas. - [Read Bisulfite-PCR for Restriction Analysis and/or Sequencing]

Bisulfite-PCR for Restriction Analysis and/or Sequencing Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=8E0AAAAC-FA42-486C-A93F60CD6E1C7E51&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: PCR Protocols: Bisulfite Conversion Based PCR Protocols

Protocol for bisulfite-PCR for restriction analysis and/or sequencing. Bisulfite-PCR followed by restriction is a rapid and semi-quantitative method of analyzing DNA methylation. The PCR products are also suitable for either direct sequencing or cloning and sequencing. The most important step here is primer selection. - [Read Bisulfite-PCR for Restriction Analysis and/or Sequencing Protocol]

Dewhurst Lab Protocols in Phage Display - http://128.151.62.33/pdf/DEWphage01.pdf

Category: Peptide Protocols: Phage Display Cloning Systems and Protocols

Dewhurst Lab Protocols in Phage Display. - [Read Dewhurst Lab Protocols in Phage Display]

Differential Display-PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3844

Category: PCR Protocols

Method uses PCR to amplify and display many cDNAs derived from the mRNAs of a given cell or tissue type. The method relies on two different types of synthetic oligonucleotides: anchored antisense primers and arbitrary sense primers. A typical anchored primer is complementary to approx. 13 nucleotides of the poly(A) tail of mRNA and the adjacent two nucleotides of the transcribed sequence. - [Read Differential Display-PCR Protocol]

DNA-Methyltransferase Assay - Anderson Centre - http://www.mdanderson.org/departments/methylation/display.cfm?id=9B5B6082-C53A-4A86-BEE3EE149A52251E&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

M.D. Anderson Cancer Centre Texas - [Read DNA-Methyltransferase Assay - Anderson Centre]

DNA-Methyltransferase Assay Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=9B5B6082-C53A-4A86-BEE3EE149A52251E&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for DNA-methyltransferase assay. Includes: Homogenize Cells/Tissues; Quantitate Protein Concentration; Dilute Samples; The Assay Proper; Troubleshooting. - [Read DNA-Methyltransferase Assay Protocol]

EMBL Cloning protocols - http://www.embl-hamburg.de/display?file=~geerlof/webPP/protocoldb/Cloning/pdb_cloning.html

Category: DNA Protocols: DNA Cloning

Preparation of oligo solutions PCR experiments, Digestion of insert DNA, Digestion and dephosphorylation of vector DNA, Ligation of DNA fragments with sticky ends, Ligation of DNA fragments with blunt ends, Preparation of chemically competent E. coli - [Read EMBL Cloning protocols]

Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4125

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from blood samples. - [Read Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display]

Extraction and Purification of RNA from Tissue Samples for Fluorescent mRNA Differential Display - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4124

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from tissue samples. - [Read Extraction and Purification of RNA from Tissue Samples for Fluorescent mRNA Differential Display]

Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4123

Category: PCR Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from the tissue-cultured cells of interest. For other starting materials, such as blood samples, please see Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display. - [Read Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential]

Methylated CpG Island Amplification - http://www.mdanderson.org/departments/methylation/display.cfm?id=8A198378-986B-41FD-8778FA1399FBAB4D&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Methylated CpG Island Amplification. Protocol written by Minoru Toyota. M.D Anderson Cancer Centre Texas. - [Read Methylated CpG Island Amplification]

Methylated CpG Island Amplification Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=8A198378-986B-41FD-8778FA1399FBAB4D&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: PCR Protocols: Bisulfite Conversion Based PCR Protocols

Protocol for methylated CpG island amplification. - [Read Methylated CpG Island Amplification Protocol]

Methylation Specific PCR Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=0CB11381-59AE-4D90-AC0B343F2D646EDD&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Methylation Specific PCR Protocol. M.D Anderson Cancer Centre Texas. Protocol written by James Herman* - [Read Methylation Specific PCR Protocol]

Methylation-Specific PCR Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=0CB11381-59AE-4D90-AC0B343F2D646EDD&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for methylation-specific PCR. - [Read Methylation-Specific PCR Protocol]

Ph.D. Peptide Display Cloning System - NEB - http://www.neb.com/nebecomm/TechBulletinFiles/techbulletinE8101.pdf

Category: Peptide Protocols: Phage Display Cloning Systems and Protocols

Ph.D. Peptide Display Cloning System - NEB. The Ph.D.â„¢ Peptide Display Cloning System facilitates the display of custom peptide libraries on the surface of bacteriophage M13 as coat protein fusions, creating a physical linkage between each displayed pep - [Read Ph.D. Peptide Display Cloning System - NEB]

Ph.D.â„¢-12 Phage Display Peptide Library Kit - http://www.neb.com/nebecomm/ManualFiles/manualE8110.pdf

Category: Peptide Protocols: Phage Display Cloning Systems and Protocols

Ph.D.â„¢-12 Phage Display Peptide Library Kit NEB. Applications for Drug Discovery using Phage Display Libraries of Peptides. - [Read Ph.D.â„¢-12 Phage Display Peptide Library Kit]

Phage Display Libraries Kits - NEB - http://search.neb.com/query.html?qt=phage+display&Image1.x=0&Image1.y=0

Category: Peptide Protocols: Phage Display Cloning Systems and Protocols

Phage Display Libraries Kits - NEB (New England Biolabs) - [Read Phage Display Libraries Kits - NEB]

Removal of Genomic DNA from Total RNA for Use in Fluorescent mRNA Differential Display Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4126

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol is the second in a set of three, describing fluorescent mRNA differential display (FDD or FDDRT-PCR). For the purposes of FDD gene expression analysis, as well as any other RNA-based gene expression technologies, contaminating genomic DNA must be removed before reverse transcription and subsequent PCR. - [Read Removal of Genomic DNA from Total RNA for Use in Fluorescent mRNA Differential Display Protocol]

Targeted Differential Display Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/RNA/TargetedDifferentialDisplay.html

Category: Primary Cell Isolation and Culture Protocols

The protocol is one example of differential gene expression analysis of cells obtained from microdissected tissue. Includes: Microdissection and RNA Isolation; Reverse Transcription; PCR; P.A.G.E.; Sequencing of Differentially Expressed Bands. - [Read Targeted Differential Display Protocol]

Articles (1-3 of 3)

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.