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Diabetes Post - http://www.diabetespost.com

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

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An Image-Based Assay of Endothelial Cell Tube Formation - http://www.bdbiosciences.com/pdfs/whitePapers/06-A790030-3A1.pdf

Category: Signalling Signal Transduction Protocols

Protocol on an image-based assay of endothelial cell tube formation as a model of angiogenesis. Includes: Introduction, Methods, Results, Discussion and References. - [Read An Image-Based Assay of Endothelial Cell Tube Formation]

Calcium Phosphate Transfection Method - http://www.flemingtonlab.com/Protocols/CalciumPhosphateTransf.pdf

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This calcium phosphate transfection method works best in cell lines that are 1) highly transformed and 2) adherent (Hela, U2OS, SAOS2, AdAH, NPC-KT and obtain from 20% to 100% transfection efficiency depending on the cell line). Works well for transient experiments but precautions should be used in the design and interpretation of experiments based on the discussion below. Also works very well for generating stable cell lines. This method is quite sensitive to the amount of input plasmid. - [Read Calcium Phosphate Transfection Method]

Column Chromatography Protocol PDF - http://www.tulane.edu/~wiser/methods/handouts/class/09_chrom.pdf

Category: Chromatography Protocols: Column Chromatography Protocols

Excellent PDF on Column Chromatography including detailed protocol and discussion. Univ. Tulane. - [Read Column Chromatography Protocol PDF]

Vectors and Agrobacterium Hosts for Arabidopsis Transformation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.ip29

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

A. tumefaciens is a soil-dwelling bacterium that transforms normal plant cells into tumor-forming cells by inserting a piece of bacterial DNA (the transfer, or "T," DNA) into the plant cell genome. The Ti plasmid also carries many of the transfer functions for mobilizing the T-DNA. This article provides a brief discussion of the principles of T-DNA transformation, including consideration of T-DNA vectors and their hosts. - [Read Vectors and Agrobacterium Hosts for Arabidopsis Transformation Protocol]