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Directed Orientation of Antibodies during Preparation of Antibody Affinity Resin Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4204

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Protocol describes the directed coupling of antibody to protein G-agarose via its Fc domain, and subsequent covalent cross-linking of the complex using dimethyl pimelimidate (DMP). - [Read Directed Orientation of Antibodies during Preparation of Antibody Affinity Resin Protocol]

Directed Yeast Two Hybrid Screening for MEKK1 Interaction Partners Protocol - http://www.natureprotocols.com/2007/01/22/directed_yeast_two_hybrid_scre.php

Category: Yeast Protocols: Yeast Two-Hybrid Systems Protocols

Protocol utilizes the Y190 yeast strain, that characteristically turns from white to red during growth due to ademutation. Baits were subcloned into the pAS1cyh2 vector, and Preys subcloned into pACT2. - [Read Directed Yeast Two Hybrid Screening for MEKK1 Interaction Partners Protocol]

Fluorescent In Situ Transcription in Cells and Tissues Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6639A7FEDD7A49BBC3758516F99557&objectid=6677C9E8B913BFB72E9F654025F3C39A

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Method assesses cellular mRNA transcripts in tissue sections and cell cultures using unique short anti-sense primers directed against sequences in particular protein(s). The unlabeled synthetic cDNA oligonucleotide primers are extended complementary to a sense mRNA transcript using reverse transcriptase and labeled through incorporation of a fluorescent-labeled dUTP nucleotide base. This procedure provides rapid detection of low abundance mRNA messages that can be related to other cellular.... - [Read Fluorescent In Situ Transcription in Cells and Tissues Protocol]

FP-TDI Method for SNP Detection - http://las.perkinelmer.com/content/snps/fp-tdi.asp

Category: DNA Protocols: SNP Protocols

FP-TDI Method for SNP Detection. The FP-TDI protocol was originally reported by Drs. Chen, Levine, and Kwok at Washington University in 19991,2. FP-TDI stands for template directed dye terminator incorporation assay with detection by fluorescence polarization. It is a single base primer extension assay couple with homogeneous FP detection. Perkin Elmer - [Read FP-TDI Method for SNP Detection]

Large-scale Preparation of Single-stranded and Double-stranded Bacteriophage M13 DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3284

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

Protocol used chiefly to generate large stocks of double-stranded DNA of strains of M13 that are routinely used as cloning vectors. Large amounts of single-stranded DNA of an individual recombinant may occasionally be needed for specific purposes, e.g., to generate many preparations of a particular radiolabeled probe or to construct large numbers of site-directed mutants. - [Read Large-scale Preparation of Single-stranded and Double-stranded Bacteriophage M13 DNA Protocol]

Oligonucleotide-directed Mutagenesis by Elimination of a Unique Restriction Site (USE Mutagenesis) - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3742

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for oligonucleotide-directed mutagenesis by elimination of a unique restriction site (USE mutagenesis). - [Read Oligonucleotide-directed Mutagenesis by Elimination of a Unique Restriction Site (USE Mutagenesis)]

Oligonucleotide-directed Mutagenesis of Single-stranded DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3817

Category: Cloning Protocols: Mutagenesis Protocols

Protocool for oligonucleotide-directed mutagenesis of single-stranded DNA. - [Read Oligonucleotide-directed Mutagenesis of Single-stranded DNA Protocol]

Preparation of Uracil-containing Single-stranded Bacteriophage M13 DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3811

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

Single-stranded templates of bacteriophage M13 DNA containing 20-30 residues of uracil in place of thymine are generated during growth of the bacteriophage in an F' strain of E. coli carrying mutations in the ung and dut genes. This DNA is used as a template in the Kunkel method of oligonucleotide-directed mutagenesis (Oligonucleotide-directed Mutagenesis of Single-stranded DNA). - [Read Preparation of Uracil-containing Single-stranded Bacteriophage M13 DNA Protocol]

Rapid Amplification of 5' cDNA Ends 5'-RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3989

Category: PCR Protocols: cDNA Synthesis Protocols

This method is used to extend partial cDNA clones by amplifying the 5' sequences of the corresponding mRNAs. The technique requires knowledge of a small region of sequence within the partial cDNA clone. During PCR, the thermostable DNA polymerase is directed to the appropriate target RNA by a single primer derived from the region of known sequence. - [Read Rapid Amplification of 5' cDNA Ends 5'-RACE Protocol]

Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP) - http://nar.oxfordjournals.org/cgi/reprint/33/21/e185?ijkey=XINgS7zgWnaIZMJ&keytype=ref

Category: RNA Protocols: cDNA Libraries Library

Hoskins et al. 2005 NAR. "... describe a PCR-based method for directed screening of plasmid cDNA libraries. " - [Read Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP)]

Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3467

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for rapid and efficient site-directed mutagenesis by the single-tube megaprimer PCR method. - [Read Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method Protocol]

Rapid PCR Site-Directed Mutagenesis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4144

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for rapid PCR site-directed mutagenesis. - [Read Rapid PCR Site-Directed Mutagenesis Protocol]

Removal of Cross-reactive Antibodies from Antiserum: Pseudoscreening Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3772

Category: Antibody Protocols: Antibody Production Protocols

Antibodies directed against bacterial and phage-encoded proteins can be removed from sera by incubation with nitrocellulose filters impregnated with lysates of uninfected or phage-infected E. coli. - [Read Removal of Cross-reactive Antibodies from Antiserum: Pseudoscreening Protocol]

Screening Recombinant Clones for Site-directed Mutagenesis by Hybridization to Radiolabeled Oligos - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4013

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

Plaques formed by M13 bacteriophages or bacterial colonies transformed by plasmids carrying specific mutations can be detected by hybridization, using a radiolabeled oligonucleotide that forms a perfect duplex with the mutant sequence. Hybridization is carried out under conditions of low stringency that allow the radiolabeled oligonucleotide to anneal to both mutant and wild-type DNAs. - [Read Screening Recombinant Clones for Site-directed Mutagenesis by Hybridization to Radiolabeled Oligos]

Site Directed Mutagenesis Protocol - http://openwetware.org/wiki/Knight:Site-directed_mutagenesis

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for site directed mutagenesis. - [Read Site Directed Mutagenesis Protocol]

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Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.