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Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols
Protocol describes the directed coupling of antibody to protein G-agarose via its Fc domain, and subsequent covalent cross-linking of the complex using dimethyl pimelimidate (DMP). - [Read Directed Orientation of Antibodies during Preparation of Antibody Affinity Resin Protocol]
Protocol describes how antibodies are bound to protein A or protein G beads and are then crosslinked to the matrix via a bifunctional coupling reagent called dimethyl pimelimidate (DMP). - [Read Immunoaffinity Purification: Coupling Antibodies to Protein A or G Bead Columns Protocol]
This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.
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