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Isolation and Culture of Mouse Bone Marrow-Derived Macrophages Protocol - http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=572&criteria=

Category: Immunology: Mononuclear Cell Isolation Protocols

Mononuclear phagocyte progenitor cells derived from femoral and tibial bone marrow are propagated in the presence of M-CSF. This macrophage growth factor is secreted by L929 cells and is used in the form of L929 cell conditioned medium. The progenitor cells proliferate and differentiate through monoblast, promonocyte and monocyte stages before maturing to macrophages. At this time the cells become firmly adherent to the culture vessel. - [Read Isolation and Culture of Mouse Bone Marrow-Derived Macrophages Protocol]

Differentiation of Embryonic Stem (ES) Cells Using the Hanging Drop Method - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4485

Category: Stem Cell Protocols: Stem Cell Culture Protocols

In vitro differentiation of ES cells occurs when the cells are allowed to aggregate in suspension culture in the absence of mouse embryonic fibroblast (MEF) feeders and leukemia inhibitory factor (LIF). Hanging drops provide a uniform aggregate size, which is then expanded by continued growth in suspension culture. The embryoid bodies are then plated and allowed to differentiate further in culture. - [Read Differentiation of Embryonic Stem (ES) Cells Using the Hanging Drop Method]

Counterstains Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4338

Category: Cell Culture Protocols: Cell Staining Protocols

Counterstains are used to help differentiate the various cell types of subcellular structures seen in cell staining. They are essential for tissue sections, allowing the identification of the cell types, but also may be helpful in other staining reactions. - [Read Counterstains Protocol]

Differentiate ES cells into cardiac myocytes - http://www.mshri.on.ca/nagy/Protocols/cardiac.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Differentiate ES cells into cardiac myocytes. Nagy Lab - [Read Differentiate ES cells into cardiac myocytes]

Differentiate ES cells into glial cells and neurons - http://www.mshri.on.ca/nagy/Protocols/neurdif.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Differentiate ES cells into glial cells and neurons. Nagy Lab. - [Read Differentiate ES cells into glial cells and neurons]

Differentiate ES cells into cystic embryoid bodies - http://www.mshri.on.ca/nagy/Protocols/cystemb.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Differentiate ES cells into cystic embryoid bodies. Nagy Lab - [Read Differentiate ES cells into cystic embryoid bodies]

Separating Postimplantation Germ Layers Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4368

Category: Stem Cell Protocols: Stem Cell Isolation Protocols

Germ layers are multipotent tissues that have the ability to differentiate into various tissue types. Isolation and manipulation of germ layers is important for examining differentiation. This protocol describes a method for separating postimplantation germ layers. - [Read Separating Postimplantation Germ Layers Protocol]

Articles (1-5 of 5)

Mast Cell Staining Protocol

A simple and concise mast cell staining protocol for cell histology.

Bismarck Brown Stain

Bismarck Brown Stain.

Crystal Violet Stain

Crystal Violet stain preparation method and protocol.

Thionin Stain

Thionin staining method and protocol for cell or tissue histology.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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