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Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

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High-Throughput Immunostaining Protocol - http://openwetware.org/wiki/Silver:_High-throughput_immunostaining

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for high-throughput immunostaining. Includes: [edit] Detection of 14-3-3 in U2OS and HeLa cells by immunofluorescence. - [Read High-Throughput Immunostaining Protocol]

Homogeneous Caspase 3-7 Assay Protocol - http://www.promega.com/tbs/tb295/tb295.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Homogeneous caspase-3/7 assay. Includes: Detection of Caspase-3/7 Activity in Cell Culture; Detection of Caspase-3 or -7 Activity in Purified Caspase Preparations; Positive and Negative Cell Culture Controls. - [Read Homogeneous Caspase 3-7 Assay Protocol]

Human T Cell Activation Protocol - http://www.ebioscience.com/ebioscience/appls/AC145.htm#human

Category: Immunology: T Cell Protocols

Protocol for the Stimulation of human peripheral blood mononuclear cells with anti-human CD3 monoclonal antibody; MTT assay for detection of cellular proliferation. Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. Performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. - [Read Human T Cell Activation Protocol]

Hybridization and Detection Using the HC ExpressArray Kit Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4122

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol describes the direct detection of RNA on DNA microarrays using Hybrid Capture (HC) technology and the HC ExpressArray Kit developed by Diagene. The kit uses a proprietary antibody that binds specifically to RNA:DNA hybrids and a second, fluorescently labeled, antibody that detects the primary antibody. Total RNA is applied directly to a glass-spotted DNA microarray, and stable RNA:DNA hybrids are visualized via a Cy3-labeled secondary antibody. - [Read Hybridization and Detection Using the HC ExpressArray Kit Protocol]

Identification of Single Bacterial Cells using DIG-Labeled Oligonucleotides Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_139-142.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for the identification of single bacterial cells using DIG-labeled oligonucleotides. Includes: Organisms and growth conditions; Cell fixation and preparation of cell smears; DIG labeling of oligonucleotides with DIG-ddUTP; In situ hybridization using digoxigenin-labeled oligonucleotides; Detection of DIG-labeled oligonucleotides with fluorescently labeled anti-DIG Fab fragments; Detection of DIG-labeled oligonucleotide. - [Read Identification of Single Bacterial Cells using DIG-Labeled Oligonucleotides Protocol]

Immunoblotting: Antigen Detection Using Chemiluminescence Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4271

Category: Western Blot Protocols: Antigen Detection Methods Protocols

The blot is blocked to prevent nonspecific adsorption of the immunological reagents. Antibodies are then bound to the proteins immobilized on the membrane, and the antigen is detected by labeling the antibodies with conveniently identified tags. Common labeling methods for chemiluminescent detection include anti-immunoglobulin antibody-coupled enzymes such as horseradish peroxidase, which catalyzes the oxidation of luminol and in turn releases light. - [Read Immunoblotting: Antigen Detection Using Chemiluminescence Protocol]

Immunoblotting: Antigen Detection Using Chromogenic Methods Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4272

Category: Western Blot Protocols: Antigen Detection Methods Protocols

Immunocytochemical Identification of Proliferating Cells In Vascular Tissue - http://www.emory.edu/WILCOX/brduDAB.html

Category: Immunology: Immunocytochemistry Protocols

Using anti-BrdU, Diaminobenzadine (DAB) brown detection. Includes counterstaining protocol. - [Read Immunocytochemical Identification of Proliferating Cells In Vascular Tissue]

Immunofluorescence Detection of RNAi-Induced Protein Knockdown in Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4344

Category: Immunology: Immunofluorescence Protocols

This protocol describes the use of a specific antibody that recognizes the targeted gene product to detect RNAi-induced gene knockdown in mammalian cells. Western blot technology can be used as an alternative (see Detection of RNAi-Induced Protein Knockdown in Mammalian Cells by Western Blotting). - [Read Immunofluorescence Detection of RNAi-Induced Protein Knockdown in Mammalian Cells Protocol]

Immunofluorescence Detection of RNAi-Induced Protein Knockdown in Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4344

Category: RNAi Technology Protocols: RNAi Mammalian Cells Protocols

Protocol describes the use of a specific antibody that recognizes the targeted gene product to detect RNAi-induced gene knockdown in mammalian cells. Western blot technology can be used as an alternative. - [Read Immunofluorescence Detection of RNAi-Induced Protein Knockdown in Mammalian Cells Protocol]

Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/14/pdb.prot4779

Category: Immunohistochemistry Protocols: Cryosectioning Protocols

This protocol describes methods for the fixation, cryosectioning, and immunohistochemical detection of proteins in embryonic and adult zebrafish eyes. The methods described can easily be adapted for use on other zebrafish tissues. - [Read Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes Protocol]

Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/14/pdb.prot4779

Category: Laboratory Model Organisms Protocols

Protocol describes methods for the fixation, cryosectioning, and immunohistochemical detection of proteins in embryonic and adult zebrafish eyes. The methods described can easily be adapted for use on other zebrafish tissues. - [Read Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes Protocol]

Immunohistochemistry on Fixed, Paraffin-Embedded Sections Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/immunohistochemistryMWO.html

Category: Immunohistochemistry Protocols

Protocol for immunohistochemistry on fixed, paraffin-embedded sections. This method is widely used and applies to the detection of the overwhelming majority of antigens, with few exceptions for which enzymatic retrieval is required. The method uses a strong chelating agent, EDTA. Includes: Double indirect AP; AP Developing solution; Indirect immunohistochemistry with avidin-biotin and HRP; HRP Developing solution. - [Read Immunohistochemistry on Fixed, Paraffin-Embedded Sections Protocol]

Immunohistochemistry Protocol for γH2AX Detection - http://www.natureprotocols.com/2006/12/06/immunohistochemistry_protocol.php

Category: Immunohistochemistry Protocols

Immunohistochemistry protocol for γH2AX detection (formalin-fixed paraffin-embedded sections). - [Read Immunohistochemistry Protocol for γH2AX Detection]

Immunoperoxidase Methods for Localization of Antigens in Cultured Cells and Tissues - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662E8B08D36AFBDC097EF76E740CB1&objectid=6673CDF3E6A07EBE28C6002718B3CC7A

Category: Immunohistochemistry Protocols

Describes two methods for using the immunoperoxidase reaction to localize antigens at the electron microscope level; one for adherent cultured cells and one for tissue sections. The reaction conditions are first optimized at the light microscope level and then adapted for EM level observation. These methods allow for reliable detection of antigens at the cell surface, within the cell, and especially in membrane bounded organelles. - [Read Immunoperoxidase Methods for Localization of Antigens in Cultured Cells and Tissues]

Immunostaining Thin Layer Chromatograms Of Glycolipids - http://www.glycotech.com/protocols/Proto5.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Immunostaining thin layer chromatograms TLC is a very sensitive detection technique of functionally active carbohydrate ligands of protein receptors. Carbohydrate structures are detected in glycolipids from complex mixtures of molecules extracted from the relevant target tissue. Proteins analyzed can be antibodies, chimeric Ig proteins, selectins, lectins, toxins, and other carbohydrate binding proteins. John L. Magnani~GlycoTech Corporation, Rockville, Maryland - [Read Immunostaining Thin Layer Chromatograms Of Glycolipids]

Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Anti - http://www.jhc.org/cgi/content/full/50/4/449

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Antibodies to Cleaved Caspase 3. Gown et al, 2002 - [Read Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Anti]

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling using Paraffin Sections. - http://www.emory.edu/WILCOX/tunel.paraf.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling using Paraffin Sections. Wilcox et al. Modified Protocol. - [Read In Situ Cell Death (Apoptosis) Detection by TUNEL labeling using Paraffin Sections.]

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling, Protocol for Frozen Sections. - http://www.emory.edu/WILCOX/tunel.frzn.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling, Protocol for Frozen Sections. Wilcox et al. Modified. - [Read In Situ Cell Death (Apoptosis) Detection by TUNEL labeling, Protocol for Frozen Sections.]

In Situ Hybridization to Human Metaphase Chromosomes using DIG-, Biotin- or Fluorochrome-Labeled DNA - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_78-94.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for In situ hybridization to human metaphase chromosomes using DIG-, biotin-, or fluorochrome-labeled DNA probes and detection with fluorochrome conjugates. Includes: Pretreatment of metaphase spreads on slides; Denaturation and hybridization; Single color fluorescent detection with immunological amplification; Multicolor fluorescence in situ hybridization (Multicolor FISH); Results obtained with human metaphase chromosome spreads. - [Read In Situ Hybridization to Human Metaphase Chromosomes using DIG-, Biotin- or Fluorochrome-Labeled DNA]

In Situ Hybridization to Human Metaphase Chromosomes Using DIG-, Biotin-, or Fluorochrome-Labeled - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_78-94.pdf

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Protocol for in situ hybridization to human metaphase chromosomes using DIG-, biotin-, or fluorochrome-labeled DNA probes and detection with fluorochrome conjugates. - [Read In Situ Hybridization to Human Metaphase Chromosomes Using DIG-, Biotin-, or Fluorochrome-Labeled]

In-Situ PCR Protocol - http://wheat.pw.usda.gov/~lazo/methods/minn/chapter3.7/chapter3.7.fm.html

Category: PCR Protocols: In-situ PCR Protocols

Amplification and Detection in a Cellular Context. Methodology, in-situ detection methods, reaction Conditions, introduction. Ernest F. Retzel et al., University of Minnesota. - [Read In-Situ PCR Protocol]

In-Vitro Kinase Assay for Jnk-1/IRS Proteins - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/In_vitro%20kinase%20assay%20for%20JNK-1%20IRS%20proteins.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describing an in vitro assay on the detection of Kinase activity for Jnk-1/IRS proteins. - [Read In-Vitro Kinase Assay for Jnk-1/IRS Proteins]

Introduction to the EMSA (Gel Shift) Technique - http://www.piercenet.com/Proteomics/browse.cfm?fldID=7EDAC33E-3981-4E58-8A57-057A8280C68F

Category: DNA Protocols: EMSA DNA-Protein Protocols

A non-radioactive electrophoretic mobility which works quite well using biotin and streptavidin detection. Non-specific and specific competititor, oligo labeling, Binding Reaction. Pierce - [Read Introduction to the EMSA (Gel Shift) Technique]

ISEL in Paraffin-embedded tissues - http://dir.niehs.nih.gov/dirlep/ish/isel1.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: ISEL

Detection of Apoptosis in Paraffin-embedded tissues by ISEL (In Situ End Labeling). Wijsman et al., 1993. - [Read ISEL in Paraffin-embedded tissues]

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Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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