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Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

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Caspase 8 Assay Protocol - http://www.promega.com/tbs/tb332/tb332.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for caspase 8 assay. Includes: Detection of Caspase-8 Activity Using Purified Caspases; Detection of Caspase-8 Activity in Cell-Based Assays. - [Read Caspase 8 Assay Protocol]

Caspase 9 Assay Protocol - http://www.promega.com/tbs/tb333/tb333.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for caspase 9 assay. Includes: Detection of Caspase-9 Activity Using Purified Caspases; Detection of Caspase-9 Activity in Cell-Based Assays. - [Read Caspase 9 Assay Protocol]

Caspase Detection Methods for Apoptosis - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/chap11.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Caspase Detection Methods for Apoptosis. Zeuner et al. Purdue Cytometry CD-ROM Series. - [Read Caspase Detection Methods for Apoptosis]

CD90 (Thy-1) Antibody Staining Protocol for Immunohistochemistry - http://www.ihcworld.com/_protocols/antibody_protocols/cd90_pharmingen.htm

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for CD90 (Thy-1) antibody staining for Immunohistochemistry. Includes: Primary Antibody; Antigen Retrieval; Detection Methods; Chromogen Substrate; Counterstain. - [Read CD90 (Thy-1) Antibody Staining Protocol for Immunohistochemistry]

Cell Viability Assays that Measure ATP Protocol - http://www.promega.com/paguide/chap4.htm#title2

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method to determine the number of viable cells in culture. Detection is based on using the luciferase reaction to measure the amount of ATP from viable cells. The amount of ATP in cells correlates with cell viability. - [Read Cell Viability Assays that Measure ATP Protocol]

CGH Protocols - http://amba.charite.de/cgh/protocol/02/prot02.html

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

A nice map linking to protocols for CGH Comparative Genomic Hybridization. Hybridization protocols, detection and analysis. Institut fÃ¼r Pathologie University Hospital CharitÃ© - [Read CGH Protocols]

Collection of Specimens for Detection of Measles Virus RNA by PCR Protocol - http://www.vidrl.org.au/labsandunits/measles/forms/Specimen%20protocol%20OCT%2003.pdf

Category: PCR Protocols

Protocol for collection of specimens for detection of measles virus RNA by PCR. - [Read Collection of Specimens for Detection of Measles Virus RNA by PCR Protocol]

Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative - http://info.med.yale.edu/genetics/ward/tavi/CCKmethod.html

Category: Cytogenetics Protocols

Protocol for color changing karytoping (CCK): an M-FISH/SKY alternative. Includes: Slide preparation and hybridization; Probes, dyes and antibodies; Probe detection and image capture; Image processing. - [Read Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative]

Combined DNA In Situ Hybridization and Immunocytochemistry Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_127-134.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Protocol for combined DNA in situ hybridization and immunocytochemistry for the simultaneous detection of nucleic acid sequences, proteins, and incorporated BrdU in cell preparations. Includes: Cell preparations and BrdU labeling; Detection of antigen by immunocytochemistry (ICC); Visualization of ICC antigen; -Gal-BCIG reaction (for producing a blue precipitate visible under brightfield microscopy); Cell processing for in situ hybridization; In situ hybridization (ISH); etc... - [Read Combined DNA In Situ Hybridization and Immunocytochemistry Protocol]

COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL - http://users.ugent.be/~fspelema/neubla/protocol/cgh.htm

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

SAVING OF DAPI-IMAGES, SLIDE PRETREATMENT, PROBE PREPARATION, PROBE DETECTION, washing blocking detection, Counterstaining with DAPI. Institute of Pathology,Humboldt-University of Berlin - [Read COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL]

Comparative Genomic Hybridization PDF - http://www.utoronto.ca/cancyto/protocols_software/CGH_protocol.pdf

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

Excellent Detailed CGH Comparative Genomic Hybridization Protocol. Squire Lab, Univ. Toronto. Includes: Labeling, Gel Interpretation, Slide Pre-Treatment and Denaturation, Probe Denaturation, Hybridization and Post Hybridization Washes and Detection, Tips - [Read Comparative Genomic Hybridization PDF]

Cytochemical Methods for the Detection of Apoptosis - http://www.jhc.org/cgi/content/full/47/9/1101

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Cytochemical Methods for the Detection of Apoptosis. Mark C. Willingham 1999 - [Read Cytochemical Methods for the Detection of Apoptosis]

Cytokine ELISA Protocol - http://www.bdbiosciences.com/pharmingen/protocols/Cytokine_ELISA.shtml

Category: Signalling Signal Transduction Protocols: Cytokine Protocols

Capture antibody, Blocking, Standards and Samples, Detection antibody, Cytokine ELISA Helpful Hints. BD Biosciences. - [Read Cytokine ELISA Protocol]

Detect and Quantitate MicroRNA in Laser Capture Microdissection Samples - http://www.ambion.com/techlib/tn/131/6.html

Category: RNAi Technology Protocols

Information on how detect and quantitate MicroRNA in laser capture microdissection samples. Includes: Obtain Laser Capture Microdissected Samples; Isolate miRNA from LCM Samples; Quantitate miRNA by qRT-PCR; Detection of miRNA in Microdissected Tissue from Mouse Brain by qRT-PCR; Differential Expression of MicroRNA in Whole Brain Tissue Compared to a More Homogeneous Population of Cells. - [Read Detect and Quantitate MicroRNA in Laser Capture Microdissection Samples]

Detection and Measurement of Radioactivity - http://www.ruf.rice.edu/%7Ebioslabs/methods/radioisotopes/rad1.html

Category: Radioactivity

Radioactive Decay, Methods of detection, Liquid scintillation counting, Background and Quenching, The scintillation counter printout. David R. Caprette, Rice Univ. - [Read Detection and Measurement of Radioactivity]

Detection FISH Protocol - http://www.riedlab.nci.nih.gov/publications/FISH%20Detection.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for Detection (FISH). TRITC, or Avidin Cy-5. For the probes labeled with digoxigenin, we usually first incubate with mouse-anti-digoxigenin, followed by incubation with sheep anti-mouse Cy5.5, or other fluorochrome conjugated antibodies. - [Read Detection FISH Protocol]

Detection of Alu by PCR - http://www.accessexcellence.com/AE/AEPC/DNA/detection.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Detection of Alu by PCR - A Human DNA Fingerprinting Lab Protocol - 1994 Cold Spring Harbor Laboratory DNA Learning Center - [Read Detection of Alu by PCR]

Detection of Alu by PCR - http://www.accessexcellence.com/AE/AEPC/DNA/detection.html

Category: PCR Protocols

Detection of Alu by PCR - A Human DNA Fingerprinting Lab Protocol - 1994 Cold Spring Harbor Laboratory DNA Learning Center - [Read Detection of Alu by PCR]

Detection of an advanced glycosylation product bound to protein in situ. PDF - http://www.jbc.org/cgi/reprint/260/13/7970.pdf

Category: Molecular Biology Protocols: Carbohydrate Protocols

Detection of an advanced glycosylation product bound to protein in situ. PDF . Chang JC, Ulrich PC, Bucala R, Cerami A. - [Read Detection of an advanced glycosylation product bound to protein in situ. PDF]

Detection of Apoptosis vs Necrosis (Oncosis) - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/chap10.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Detection of Apoptosis vs Necrosis (Oncosis). Amazing review of methods. Vitale et al. - [Read Detection of Apoptosis vs Necrosis (Oncosis)]

Detection of apoptotic process in situ using immunocytochemical - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/barbie.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Detection of apoptotic process in situ using immunocytochemical and TUNEL assays. Barbieri et al. Purdue Cytometry CD-ROM Series - [Read Detection of apoptotic process in situ using immunocytochemical]

Detection of Autoantibodies with Self-Assembling Radiolabeled Antigen Tetramers Protocol - http://www.natureprotocols.com/2007/01/29/detection_of_autoantibodies_wi.php

Category: Antibody Protocols: Antibody Labeling Protocols

Protocol for the detection of autoantibodies with self-assembling radiolabeled antigen tetramers. - [Read Detection of Autoantibodies with Self-Assembling Radiolabeled Antigen Tetramers Protocol]

Detection of Autoantibodies with Self-Assembling Radiolabeled Antigen Tetramers Protocol - http://www.natureprotocols.com/2007/01/29/detection_of_autoantibodies_wi.php

Category: Immunology

Protocol for detection of autoantibodies with self-assembling radiolabeled antigen tetramers. Details how to produce radiolabeled antigen-streptavidin tetramers for detection of antibodies by immunoprecipitation. Optionally, the antigen tetramers can be denatured to compare responses to folded and unfolded antigen in the same system. This technique can be applied to a large or small number of samples, and a given sample can be simultaneously assayed with multiple antigens. - [Read Detection of Autoantibodies with Self-Assembling Radiolabeled Antigen Tetramers Protocol]

Detection of ß-Galactosidase and Alkaline Phosphastase Activities in Tissue Protocol - http://genetics.med.harvard.edu/~cepko/protocol/xgalplap-stain.htm

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for detection of ß-Galactosidase and alkaline phosphastase activities in tissue. - [Read Detection of ß-Galactosidase and Alkaline Phosphastase Activities in Tissue Protocol]

Detection of BrdU Incorporation in DNA Synthesizing Cells Protocol - http://www.bdbiosciences.com/pharmingen/protocols/BrdU_Incorporation.shtml

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Protocol for the detection of BrdU incorporation in DNA synthesizing cells. - [Read Detection of BrdU Incorporation in DNA Synthesizing Cells Protocol]

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Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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