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A Method for Assaying Deubiquitinating Enzymes - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=140113

Category: Protein Protocols: Protein Ubiquitination Protocols

A Method for Assaying Deubiquitinating Enzymes. Lee et al., Biol Proced Online. May 1998. A general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused Î±NH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. - [Read A Method for Assaying Deubiquitinating Enzymes]

In Vivo Functional Real-Time Imaging of Transplanted Islets Using Positron Emission Tomography (PET) - http://www.natureprotocols.com/2006/12/21/seeing_is_believing_in_vivo_fu.php

Category: Microscopy Protocols: In Vivo Imaging Protocols

Positron emission tomography (PET) is a established quantitative and noninvasive imaging modality. With the PET reporter gene (PRG)/PET reporter probe (PRP) system, based on a mutant form of herpes simplex virus 1 thymidine kinase (HSV1-sr39tk), the PET signal is directly proportional to the enzymatic activity of sr39TK9-14. In this protocol, we describe in detail a method for reporter gene labeling of islets and quantitative scanning using a reporter probe. - [Read In Vivo Functional Real-Time Imaging of Transplanted Islets Using Positron Emission Tomography (PET)]

Measurement of Intracellular Ions by Flow Cytometry Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C4C9AC9935B31A1C2087E2276A3&objectid=6674EF32EE59A37C48B4A267D733CAE2

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Describes flow cytometric protocols using the dyes Indo-1 AM, Fluo-3, and Fura Red AM to measure intracellular calcium concentration. Support protocols detail the use of calcium buffers to calibrate a flow cytometric calcium assay, and methods to facilitate dye loading; an alternate protocol describes the use of a spectrofluorimeter to measure intracellular calcium for those investigators without access to a flow cytometer. - [Read Measurement of Intracellular Ions by Flow Cytometry Protocol]

Murine Osteoblast Harvest Protocol - http://www.labprotocol.com/detail.php?siteid=32

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol for murine osteoblast harvest. - [Read Murine Osteoblast Harvest Protocol]

Neural Stem Cell Culture: Neurosphere Generation, Microscopical Analysis and Cryopreservation - http://www.natureprotocols.com/2006/08/25/neural_stem_cell_culture_neuro.php

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Describes the steps in detail to isolate and expand neural stem cells in the form of neurospheres from tissue dissections of the post-natal mouse brain. Procedures for the long term passage of neurospheres and the cryopreservation of neurospheres are also provided. In addition to the guidelines and tips for generating neurosphere cultures, we describe the method to prepare neurospheres for analysis by light microscopy. - [Read Neural Stem Cell Culture: Neurosphere Generation, Microscopical Analysis and Cryopreservation]

Osteoblast Harvest (murine) Protocol - http://www.labprotocol.com/detail.php?siteid=32

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Protocol for osteoblast harvest (murine). - [Read Osteoblast Harvest (murine) Protocol]

Phosphotungstic Acid Haematoxylin Stain for Fine Cell Detail Protocol - http://www.nottingham.ac.uk/pathology/protocols/ptah.html

Category: Neuroscience Protocols: Histology Stains Protocols: Hematoxylin Eosin Staining Protocols

Protocol for Phosphotungstic acid- Haematoxylin stain for fine cell detail. - [Read Phosphotungstic Acid Haematoxylin Stain for Fine Cell Detail Protocol]

Transgenic - Universal Mouse Genotyping Protocol Using PCR - http://www.knockoutmousestation.com/mouse/detail/link-28.html

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

Description:, Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core. ... - [Read Transgenic - Universal Mouse Genotyping Protocol Using PCR]

Articles (1-3 of 3)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.