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Can you autoclave liquid Media? FAQ - http://invitrogen.custhelp.com/cgi-bin/invitrogen.cfg/php/enduser/std_adp.php?p_sid=HczfJTng&p_lva=1&p_faqid=120

Category: Cell Biology and Cell Culture: Cell Culture Growth Media

Most media cannot be autoclaved. With description. Invitrogen - [Read Can you autoclave liquid Media? FAQ]

Competent cells, calcium chloride method, E. coli, description - http://web.uct.ac.za/depts/mmi/bbhelp/bac1.html

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Competent cells, calcium chloride method, E. coli, description. CaCl2 and DMSO methods for preparing bacterial and E.Coli competent cells. Zappe, H. BBHelp. - [Read Competent cells, calcium chloride method, E. coli, description]

Cytokine ELISPOT Protocol - http://www.ebioscience.com/ebioscience/appls/ELISPOT.htm#protocol

Category: ELISA Protocols: ELISpot Protocols

Protocol for cytokine ELISPOT. Includes: Description; Stability; Contents of the ELISPOT Ready-SET-Go! Reagent Set; ELISPOT Method; Cytokine ELISPOT Buffers. - [Read Cytokine ELISPOT Protocol]

DNA Ligation - http://www.bio.com/protocolstools/protocol.jhtml?id=p2053

Category: Molecular Biology Protocols: DNA Ligation Protocols

Description and protocol of the steps required to join together plasmid and insert fragments to create a new plasmid. Paul Kaufman, Univ California. - [Read DNA Ligation]

Dye Protocols and Notes for Cosmid, BAC, BAC, Fosmid Templates - http://www.genome.ou.edu/big_dyes_cos_bac_pac_fos.html

Category: BACs Bacterial Artificial Chromosome Protocols

Sequencing conditions tested for the ABI Big-Dye terminators (PE-ABI #4303150 for the 1000 reaction kit - Description: TF,KIT BTD RR-1000) with various templates. Important to quantitate all templates by agarose gel electrophoresis vs size and concentration standards and do a few tests with different template concentrations to determine the optimal conditions for your reactions. Several conditions are given. - [Read Dye Protocols and Notes for Cosmid, BAC, BAC, Fosmid Templates]

Electrophysiology - The Patch Clamp Technique - http://www.bio.psu.edu/People/Faculty/Assmann/Lab/techniques.html

Category: Electrophysiology Ion Channels

Electrophysiology - The Patch Clamp Technique. Pictures and description. Assmann - [Read Electrophysiology - The Patch Clamp Technique]

FPLC Protocol - http://users.path.ox.ac.uk/~ciu/Methods/Protein/fplc.html

Category: Chromatography Protocols

FPLC Protocol. The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting. For use of individual columns follow the "instructions" (in the green folder) which accompany each one. Sir William Dunn School of Pathology, Oxford University. - [Read FPLC Protocol]

Laser-capture Microdissection Protocol - http://www.nature.com/nprot/journal/v1/n2/full/nprot.2006.85.html#a1

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity (LOH) analysis, etc. Protocol provides a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1–1.5 h. - [Read Laser-capture Microdissection Protocol]

Live Cell Spinning Disk Confocal Fluorescence Imaging of Cells- YFP & Bright Field—Three Z Axis - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000144.pdf?pid=PP00000144

Category: Microscopy Protocols: Confocal Microscopy Protocols

Protocol describes the acquisition and processing of confocal fluorescent and bright field images of live cells expressing yellow fluorescent protein (YFP), with a spinning disk confocal head on a Zeiss Axiovert 200 M microscope when three planes along the z-axis of the cell are acquired. Protocol includes: Description of Microscope and Imaging Setup; Description of Acquisition Parameters; Image Processing. - [Read Live Cell Spinning Disk Confocal Fluorescence Imaging of Cells- YFP & Bright Field—Three Z Axis]

Live Cell Spinning Disk Confocal Fluorescence Imaging of Cells- YFP and Bright Field Images - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000145.pdf?pid=PP00000145

Category: Microscopy Protocols: Confocal Microscopy Protocols

Protocol describes the acquisition and processing of confocal fluorescent and bright field images of live cells expressing yellow fluorescent protein (YFP), with a spinning disk confocal head on a Zeiss Axiovert 200 M microscope. Protocol includes: Description of Microscope and Imaging Setup; Description of Acquisition Parameters; Image Processing. - [Read Live Cell Spinning Disk Confocal Fluorescence Imaging of Cells- YFP and Bright Field Images]

Live Cell Spinning Disk Confocal Fluorescence Imaging of Cells- YFP Time Series for Markers - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000146.pdf?pid=PP00000146

Category: Microscopy Protocols: Confocal Microscopy Protocols

Protocol describes the acquisition and processing of confocal fluorescent images of live cells expressing yellow fluorescent protein (YFP), with a spinning disk confocal head on a Zeiss Axiovert 200 M microscope. Protocol includes: Description of Microscope and Imaging Setup; Description of Acquisition Parameters; Movie Processing. - [Read Live Cell Spinning Disk Confocal Fluorescence Imaging of Cells- YFP Time Series for Markers]

Microarray Analysis of Highly Purified Human Decidual NK Cells Protocol - http://www.natureprotocols.com/2006/08/07/microarray_analysis_of_highly.php

Category: Microarray Protocols: Microarray Analysis Protocols

Description of gene array analysis and normalization - [Read Microarray Analysis of Highly Purified Human Decidual NK Cells Protocol]

Microinjection of dsRNA into Drosophila Embryos Protocol - http://www.cshprotocols.org/cgi/content/full/2006/21/pdb.prot4516

Category: Microinjection Protocols: Microinjection of DNA Protocols

This protocol provides a description of how to introduce double-stranded RNA (dsRNA) into Drosophila embryos by microinjection. Several days of preparation are required before injections into Drosophila embryos begin. Flies must be in abundant supply for egg collection. Bombardment of embryos with dsRNA-coated gold particles (Delivery of dsRNA into Drosophila Embryos by a Gene Gun) can be used as an alternative. - [Read Microinjection of dsRNA into Drosophila Embryos Protocol]

Micronucleus assay Human Lymphocytes - http://ehs.sph.berkeley.edu/holland/protocolLibrary/Cytogenetics_MN_Lymphocytes.html

Category: Cytogenetics Protocols

MN in Human Lymphocytes (method and protocol description) Nina T. Holland, Division of Environmental Health Sciences, Berkeley - [Read Micronucleus assay Human Lymphocytes]

Mini-Chamber for Regulating Gaseous Environment During Culture - http://www.animal.ufl.edu/hansen/protocols/minigaschamber.htm

Category: Developmental Biology: Embryology Protocols

A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a device. - [Read Mini-Chamber for Regulating Gaseous Environment During Culture]

Protocol for Live Cell Spinning Disk Confocal Fluorescence Imaging of Cells on a Zeiss - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000135.pdf?pid=PP00000135

Category: Microscopy Protocols: Confocal Microscopy Protocols

Protocol describes the acquisition of confocal fluorescent and bright field images of live cells, expressing cyan fluorescent protein (CFP) and/or yellow fluorescent protein (YFP), with a spinning disk confocal head on a Zeiss Axiovert 200 M microscope. Protocol includes: Description of Microscope and Imaging Setup; Description of Acquisition Parameters; Image Processing; Movie Processing. - [Read Protocol for Live Cell Spinning Disk Confocal Fluorescence Imaging of Cells on a Zeiss]

Quantitative Telomerase Detection Kit - http://www.biomax.us/pdfs/TelomeraseDetectionKitManual.pdf

Category: Cell Biology and Cell Culture

Quantitative Telomerase Detection Kit. Includes: QTD Real-Time PCR Assay Procedure; Quantitative Telomerase Detection Kit Description. - [Read Quantitative Telomerase Detection Kit]

Transgenic - Universal Mouse Genotyping Protocol Using PCR - http://www.knockoutmousestation.com/mouse/detail/link-28.html

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

Description:, Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core. ... - [Read Transgenic - Universal Mouse Genotyping Protocol Using PCR]