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(PI 3-Kinase) Activity Assay - http://www.upstate.com/misc/protocol_detail.q.prot.e.assay.a.name.e.Phosphatidyl_Inositol-3_Kinase_Assay_%28PI3%20Kinase%20Assay%29

Category: Signalling Signal Transduction Protocols

Protocol describing (PI 3-Kinase) activity assay. Includes Preparation of Phosphatidylinositol (PI). - [Read (PI 3-Kinase) Activity Assay]

Agrobacterium-Mediated Gene Transfer via Hypocotyls Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/cottontr.html

Category: Plant Biology Protocols: Plant Genetics Protocols

Protocol describing Agrobacterium mediated gene transfer via hypocotyls. - [Read Agrobacterium-Mediated Gene Transfer via Hypocotyls Protocol]

Analysis of protein phosphorylation: methods and strategies PDF - http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1365-313X.2005.02613.x

Category: Protein Protocols: Protein Phosphorylation Protocols

Paper describing methods for monitoring kinase activity, investigating kinaseâ€“substrate specificity, examining phosphorylation in planta and the determination of phosphorylation sites in a protein. In addition, strategic considerations for experimental design and variables will be discussed. Scott C. Peck, the Plant Journal. - [Read Analysis of protein phosphorylation: methods and strategies PDF]

CAT Assay (Liquid Phase) Protocol - http://snbworld.com/cat2.html

Category: Reporter Gene Assays: Cat Assays Protocols

Protocol describing a CAT assay (liquid phase). - [Read CAT Assay (Liquid Phase) Protocol]

CAT Assay Protocols - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/VI.%20Transcription/G.%20CAT%20ASSAY.pdf

Category: Reporter Gene Assays: Cat Assays Protocols

Two protocols describing CAT assays. - [Read CAT Assay Protocols]

Chlorophyll Content Protocol - http://homepages.gac.edu/~cellab/chpts/chpt8/ex8-2.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol describing how to calculate chlorophyll content. - [Read Chlorophyll Content Protocol]

Culture Chambers for Live-Cell Imaging - http://www.microscopyu.com/articles/livecellimaging/culturechambers.html

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Specimen chambers have had many designs published over the years describing systems that offer excellent optical properties while allowing specimens to be maintained for varying amounts of time. Ranging in complexity from the simple preparation of a sealed coverslip on a microscope slide to sophisticated perfusion chambers that enable tight control of virtually all environmental variables culture chambers are designed to to allow living specimens to be observed with minimal invasion at high res. - [Read Culture Chambers for Live-Cell Imaging]

Cytochrome Oxidase Manometric Analysis - http://homepages.gac.edu/~cellab/chpts/chpt8/ex8-6.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol describing cytochrome oxidase manometric analysis. Protocol includes: Manometric Analysis and information on the the Gilson Constant Pressure Respirometer. - [Read Cytochrome Oxidase Manometric Analysis]

DNA Extraction Procedure (CsCl) - Rhizobium - http://wheat.pw.usda.gov/~lazo/methods/lazo/rhizodna.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol describing extraction of DNA from Rhizobium. - [Read DNA Extraction Procedure (CsCl) - Rhizobium]

Ecdysone as Regulator of Inducible Gene Expression in Mammalian Cells - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3186

Category: Reporter Gene Assays

Protocol describing ecdysone as regulator of inducible gene expression in mammalian cells. - [Read Ecdysone as Regulator of Inducible Gene Expression in Mammalian Cells]

Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4125

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from blood samples. - [Read Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display]

Extraction and Purification of RNA from Tissue Samples for Fluorescent mRNA Differential Display - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4124

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from tissue samples. - [Read Extraction and Purification of RNA from Tissue Samples for Fluorescent mRNA Differential Display]

Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4123

Category: PCR Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from the tissue-cultured cells of interest. For other starting materials, such as blood samples, please see Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display. - [Read Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential]

In-Vitro Kinase Assay for Jnk-1/IRS Proteins - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/In_vitro%20kinase%20assay%20for%20JNK-1%20IRS%20proteins.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describing an in vitro assay on the detection of Kinase activity for Jnk-1/IRS proteins. - [Read In-Vitro Kinase Assay for Jnk-1/IRS Proteins]

Isolation of Chloroplasts from Spinach Leaves Protocol - http://homepages.gac.edu/~cellab/chpts/chpt8/ex8-1.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol describing the isolation of chloroplasts from spinach leaves. - [Read Isolation of Chloroplasts from Spinach Leaves Protocol]

JNK Kinase Assay - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/JNK%20Kinase%20Assay%20LRui.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describing an JNK Kinase Assay. Protocol includes information on reaction buffer. - [Read JNK Kinase Assay]

Luciferase Assays in OT-1 Hybridoma Cells Protocol - http://www.natureprotocols.com/2007/01/29/luciferase_assays_in_ot1_hybri.php

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol describing luciferase assays in OT-1 hybridoma cells. - [Read Luciferase Assays in OT-1 Hybridoma Cells Protocol]

Molecular Analysis and Results - DNA Comparative Genomic Hybridization (CGH) - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/DNA/CGH.html

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

Based on the original protocol describing CGH by Kallioniemi et al. - [Read Molecular Analysis and Results - DNA Comparative Genomic Hybridization (CGH)]

Molecular Probes Handbook Invitrogen - http://probes.invitrogen.com/handbook/

Category: Molecular Imaging

The Handbook â€” A Guide to Fluorescent Probes and Labeling Technologies is a comprehensive resource for fluorescence technology and its applications. Newly revised, The Handbook contains detailed information describing the use of more than 3000 Molecular - [Read Molecular Probes Handbook Invitrogen]

Mutation Detection by Solid-Phase Chemical Cleavage of Mismatches Using Radioactive Probes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4120

Category: Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols

This protocol is the first of two describing solid-phase chemical cleavage of mismatch (SpCCM), a method for the detection of mutations in genomic DNA. DNA fragments up to 2 kb in length can be analyzed to determine the position (within 10-15 bp) and identity of the mismatched nucleotide. - [Read Mutation Detection by Solid-Phase Chemical Cleavage of Mismatches Using Radioactive Probes Protocol]

Photosynthesis by Respirometry - http://homepages.gac.edu/~cellab/chpts/chpt8/ex8-8.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol describing photosynthesis by respirometry. Includes characteristics of isolated chloroplasts. - [Read Photosynthesis by Respirometry]

Plant Crosses Protocol - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/PlantWork/crosses.html

Category: Plant Biology Protocols: Plant Genetics Protocols

Protocol describing on how to perform crosses in plants. - [Read Plant Crosses Protocol]

Protocol for JNK Kinase Assay - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/JNK%20Kinase%20Assay.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describing a JNK kinase asssay. Protocol includes information on: Triton Lysis Buffer, Kinase Assay Buffer, Kinase Assay and Preparation of kinase - [Read Protocol for JNK Kinase Assay]

Removal of Genomic DNA from Total RNA for Use in Fluorescent mRNA Differential Display Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4126

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol is the second in a set of three, describing fluorescent mRNA differential display (FDD or FDDRT-PCR). For the purposes of FDD gene expression analysis, as well as any other RNA-based gene expression technologies, contaminating genomic DNA must be removed before reverse transcription and subsequent PCR. - [Read Removal of Genomic DNA from Total RNA for Use in Fluorescent mRNA Differential Display Protocol]

Small Scale Plasmid Isolation for Xanthomonas - http://wheat.pw.usda.gov/~lazo/methods/lazo/xcmini.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol describing isolation of small scale plasmid from Xanthomonas. - [Read Small Scale Plasmid Isolation for Xanthomonas]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.