dept Protocols

Category: Protein Protocols: Protein Expression Protocols

Expression of F-tryptophan labelled protein. Protocol can be adapted for Fluoro-phenylalanine or Fluro-tyrosine labelling. Transformation of competent E. coli cells for expression with plasmid. Dr. Chen, Dept. of Biochem. & Mol. Biology, University College, London. - [Read Expression of F-tryptophan labelled protein]

Category: Histology Protocols: Tissue Sample Sectioning

Frozen Sectioning and Tissue Sectioning for Electron Microscopy. Includes Tissue Processing for Transmission Electron Microscopy and Tissue Preservation for Light Microscopy. University of California UCSF Dept. Pathology Research. - [Read Frozen Sectioning and Tissue Sectioning for Electron Microscopy]

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

The procedures involve the isolation and growth of primary cell cultures from rodent and human tissue as well as the use of viral vectors for the introduction and expression of mammalian genes in cells in culture and in live rodents. - [Read Growth of Primary Cell Culture and Viral Vector Handling Protocols]

Category: Mouse Protocols: Mouse Cell Culture Protocols

The procedures involve the isolation and growth of primary cell cultures from rodent and human tissue as well as the use of viral vectors for the introduction and expression of mammalian genes in cells in culture and in live rodents. - [Read Isolation and Growth of Primary Cell Cultures from Mouse Protocol]

Category: General Research Protocols and Methods: Laboratory Safety

Laboratory Safety Program - Emergency Preparedness, Autoclave Safety, Biosafety, Carcinogens, Chemical Hygiene Plan, Glass Disposal, Fire Safety, Eyewash and Safety Shower Information, Fume Hood Procedures and Practices. University of Wisconsin - Milwaukee. - [Read Laboratory Safety Program]

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay. The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-275 (1951)]. Under alkaline conditions, copper complexes with protein. When folin phenol reagent (phospho-molybdic-phosphotungstic reagent) is added, the Folin-phenol reagent binds to the protein. Bound reagent is slowly reduced and changes color from yellow to blue. P.J. Hansen, Dept. of Animal Sciences, University of Florida. - [Read Lowry Protein Assay]

Category: Luciferase and Dual Luciferase Assays

Luciferase Assay Protocol for HEK COS cells. University College Cork UCC. Dept. Physiology. - [Read Luciferase Assay Protocol for HEK COS cells]

Category: Molecular Biology Protocols: Phage Protocols and Methods

Plating Lambda Phage to Generate Plaques. Elution of Phage. Phage DNA Extraction. Dr.Wurtzel. Dept. Biological Sciences, Lehman College. - [Read Phage Titer]

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Cell Fixation. 1. Gently wash cells 2x in sterile 1XPBS, ... [Take to Research Histology for embedding] Instructions: Embed pellets in vertical ... Jena Giltnane Yale University Dept. of Pathology. Processing Adherent Cultured Cells for Tissue Microarray Controls. - [Read Processing Adherent Cultured Cells for Tissue Microarray Controls ...]

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE). Ed Rybicki and Maud Purves. Dept Microbiology, University of Cape Town. PROTOCOL includes: Assembling Gel Apparatus, Resolving Gels, Stacking Gels, Electrophoresis buffer, Sample Preparation for Sds-page and Staining of Gels. - [Read SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)]

Category: Cloning Protocols: Vector Protocols

Information on how to handle viral vectors. Includes: Adenovirus;  MoMLV retrovirus; MoMLV-based Retroviral Vectors: Base classification BSL-1. - [Read Viral Vector Handling Information]