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Bead Aggregation Assay Protocol - http://www.natureprotocols.com/2006/09/26/bead_aggregation_assay_to_demo.php

Bead aggregation assay to demonstrate presynaptic differentiation induced by the NGL family of cell adhesion molecules. - [Read Bead Aggregation Assay Protocol]

Bielschowsky Technique for Senile Plaques Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/BIELSCH.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

A silver stain to demonstrate neurofibrillary tangles, nerve fibers and senile plaques in Alzheimer's disease. The nerve fibers are sensitized with a silver solution. The sections are treated with ammoniacal silver, and then reduced to a visible metallic silver. - [Read Bielschowsky Technique for Senile Plaques Protocol]

Congo Red Putcher's Modification Amyloid Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/CONGORED.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

Protocol to demonstrate amyloid deposits in tissue sections. When stained with the Congo Red Stain the amyloid, with the aide of polarizing lenses, will birefringe an apple green color. Under the microscope. - [Read Congo Red Putcher's Modification Amyloid Protocol]

High-resolution SNP mapping by denaturing HPLC - http://www.pnas.org/cgi/content/full/99/16/10575

Category: DNA Protocols: SNP Protocols

High-resolution SNP mapping by denaturing HPLC. A SNP mapping procedure that relies on resolving polymorphisms by denaturing HPLC without the necessity of determining the nature of the SNPs. They demonstrate the use of denaturing high-performance liquid chromatography to identify mutations in the candidate genes and to fine-map chromosomal breakpoints. - [Read High-resolution SNP mapping by denaturing HPLC]

How to Demonstrate Barrage in Neurospora - http://www.fgsc.net/neurosporaprotocols/How%20to%20demonstrate%20barrage%20with%20figure.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to demonstrate barrage in Neurospora. - [Read How to Demonstrate Barrage in Neurospora]

Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation - http://www.natureprotocols.com/2006/09/26/neuronfibroblast_coculture_ass.php

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Neuron-fibroblast coculture assay to demonstrate presynaptic differentiation. Includes: Preparation of HEK293T cells, Coculture of HEK293T cells and neurons, Immunostaining, Vesicle turnover experiment, Image acquisition and quantitation. - [Read Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation]

Reverse Ligation Mediated RT - PCR - http://www.liv.ac.uk/~giles/methods/rlpcr.htm

Category: RNA Protocols: RT-PCR and cDNA synthesis

This procedure was first described by Bertrand et al to demonstrate that ribozymes could be enzymatically active in vivo. We adapted the method to show that certain oligodeoxynucleotides could direct the activity of endogenous ribonuclease H to cleave tar - [Read Reverse Ligation Mediated RT - PCR]