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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

TaqMan Assay on CYP2C9 Protocol - http://www.pharmgkb.org/do/serve?objId=PA149569824&objCls=TaqmanAssay

Category: Pharmacology and Toxicology Protocols: Drug Metabolism Protocols

Protocol for TaqMan assay on CYP2C9. - [Read TaqMan Assay on CYP2C9 Protocol]

TaqManÂ® SNP Genotyping Assays - http://www.rockefeller.edu/genomics/pdf/Applied_Biosystems_Taqman_SNP_Genotyping.pdf

Category: DNA Protocols: SNP Protocols

TaqManÂ® SNP Genotyping Assays. Applied Biosystems - [Read TaqManÂ® SNP Genotyping Assays]

Target Specific Whole Cell Assay for Antibacterial Drug Discovery Protocol - http://www.natureprotocols.com/2006/06/23/a_targetspecific_whole_cell_as.php

Category: Pharmacology and Toxicology Protocols: Drug Discovery

Protocol describes a target selective S. aureus whole cell assay that combines agar-diffusion and protein over expression techniques. This agar based two-plate differential sensitivity assay was used to help confirm the newly discovered antibiotic platensimycin inhibited bacterial growth by specifically targeting the essential FASII enzyme FabF6. - [Read Target Specific Whole Cell Assay for Antibacterial Drug Discovery Protocol]

Targeted Differential Display Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/RNA/TargetedDifferentialDisplay.html

Category: Primary Cell Isolation and Culture Protocols

The protocol is one example of differential gene expression analysis of cells obtained from microdissected tissue. Includes: Microdissection and RNA Isolation; Reverse Transcription; PCR; P.A.G.E.; Sequencing of Differentially Expressed Bands. - [Read Targeted Differential Display Protocol]

TC-100 Insect Medium Protocol - http://www.sigmaaldrich.com/img/assets/11020/TC100_Insect_Medium.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Insect Cell Culture Growth Media Protocols

Protocol for TC-100 insect medium. - [Read TC-100 Insect Medium Protocol]

TCA Acetone precipitation protocol DOC - http://caffeine.chemistry.montana.edu/mass-spec/TCA%20acetone%20ppt%20protocl..doc

Category: Protein Protocols: Protein Precipitation Procols

TCA Acetone precipitation protocol DOC. Matt Shipman. 2006. Adapted and modified from Gruehler et al., 2005. - [Read TCA Acetone precipitation protocol DOC]

TCA Protein concentration of Laemmli gel samples - http://kinase.uhnres.utoronto.ca/methods/TCA.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

TCA Protein concentration of Laemmli gel samples. Woodgett Lab. - [Read TCA Protein concentration of Laemmli gel samples]

TCA protein precipitation protocol PDF - http://www.its.caltech.edu/~bjorker/TCA_ppt_protocol.pdf

Category: Protein Protocols: Protein Precipitation Procols

Simple TCA method with recipe. TCA protein precipitation protocol. Luis Sanchez Caltech. - [Read TCA protein precipitation protocol PDF]

TCA/Acetone Precipitation (Large Scale) PDF - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000148.pdf?pid=PP00000148

Category: Protein Protocols: Protein Precipitation Procols

A protein precipitation procedure used to precipitate protein from cell lysates. Allows for optimal protein recovery and accurate assays. The Signaling Gateway - [Read TCA/Acetone Precipitation (Large Scale) PDF]

Technical information on 2-D PAGE - http://us.expasy.org/ch2d/protocols/

Category: Proteomic Protocols: 2-D Gel Electrophoresis

Very Concise Protocol for 2D Electrophoresis. ExPASY. - [Read Technical information on 2-D PAGE]

Techniques for Primary Culture of C. elegans Embryonic Neurons - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=14

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: C. elegans Neuronal Cell Culture Protocols

Development of techniques for primary culture of C. elegans embryonic neurons. - [Read Techniques for Primary Culture of C. elegans Embryonic Neurons]

Techniques for Primary Culture of C. elegans Embryonic Neurons II - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=15

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: C. elegans Neuronal Cell Culture Protocols

Development of techniques for primary culture of C.elegans embryonic neurons. - [Read Techniques for Primary Culture of C. elegans Embryonic Neurons II]

Techniques for Primary Culture of C. elegans Embryonic Neurons III - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=16

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: C. elegans Neuronal Cell Culture Protocols

Development of techniques for primary culture of C. elegans embryonic neurons - [Read Techniques for Primary Culture of C. elegans Embryonic Neurons III]

Telescopes Astronomy - http://www.telescopesastronomy.com/

Category: Affiliates

Information on telescopes and astronomy. Includes a forum for astronomy discussions. - [Read Telescopes Astronomy]

Telomerase PCR ELISA Protocol - http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.4_96/p7-8.pdf

Category: Cell Biology and Cell Culture

Protocol for telomerase PCR ELISA. Fast and sensitive detection of telomerase activity. - [Read Telomerase PCR ELISA Protocol]

Telomerase PCR ELISA Protocol - http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.4_96/p7-8.pdf

Category: ELISA Protocols

Protocol for telomerase PCR ELISA. For fast and sensitive detection of telomerase activity. - [Read Telomerase PCR ELISA Protocol]

Telomere Length Determination Protocol - http://www-rcf.usc.edu/~forsburg/telomere.html

Category: Cell Biology and Cell Culture

Telomere length can vary from strain to strain and is sensitive to a variety of mutants affecting DNA and chromosome function. Includes: Gel Protocol; Southern blot using oligo probe. - [Read Telomere Length Determination Protocol]

TEM Sample Preparation Links - http://bama.ua.edu/~hsmithso/class/bsc_656/websites/sample_tem.html

Category: Molecular Imaging: Electron Microscopy Protocols

Embedding. Diagnostic Electron Microscopy on Reembedded ("Popped Off") Areas of Large Spurr Epoxy Sections ï¿½ LR White Microwave Processing Protocol . TEM Sample Preparation Links and Protocols. - [Read TEM Sample Preparation Links]

Tenascin Expression Protein Protocol - http://www.cellbio.duke.edu/Faculty/Erickson/pdf%27s/aExpression%20&%20purif%20protoco.pdf

Category: Cell Organelle Protocols: Extracellular Matrix Protocols

Protocol relating to tenascin expression protein. - [Read Tenascin Expression Protein Protocol]

Terrific Broth Media Protocol - http://www.thelabrat.com/protocols/TerrificBroth.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols: Broth Culture Media Protocols

Protocol for terrific broth media. - [Read Terrific Broth Media Protocol]

Test Knowing How to Practice Safe Science - http://info.med.yale.edu/caim/hhmi/public/

Category: General Research Protocols and Methods: Laboratory Safety

Test Knowing How to Practice Safe Science. Yale University. A great online interactive test for lab safety. - [Read Test Knowing How to Practice Safe Science]

Testing Rabbit Bleeds By Elisa - http://mitchison.med.harvard.edu/protocols/ab2.html

Category: Immunology

Coupling Peptide to BSA, ELISA with Peptide conjugated to BSA. Claire Walczak - [Read Testing Rabbit Bleeds By Elisa]

Tetracycline as Regulator of Inducible Gene Expression III - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3958

Category: Transfection Protocols: Stable Transfection Protocols

Stably transfected cells, generated in the first two stages of the procedure, are induced for expression of the target gene. After harvesting and lysis, the lysates are analyzed by SDS-PAGE and immunoblotting. - [Read Tetracycline as Regulator of Inducible Gene Expression III]

Tetracycline as Regulator of Inducible Gene Expression Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3986

Category: Transfection Protocols: Stable Transfection Protocols

Protocol uses an autoregulatory system in which the transcriptional trans-activator tTA drives its own expression and that of a target gene. The first stage of the procedure describes how to generate stable lines of NIH-3T3 cells that express either tTA alone or tTA and the tetracycline-regulated target gene. - [Read Tetracycline as Regulator of Inducible Gene Expression Protocol]

Tetracycline as Regulator of Inducible Gene Expression Protocol II - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3957

Category: Transfection Protocols: Stable Transfection Protocols

This stage of the procedure describes the transfection with target genes of cell lines already expressing inducible tTA. In this example, the target genes are transfected on a plasmid that carries puromycin resistance as a selectable marker. - [Read Tetracycline as Regulator of Inducible Gene Expression Protocol II]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.