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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Vectorette PCR of Yeast DNA Protocol - http://genomics.princeton.edu/botstein/protocols/vectorette.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for vectorette PCR of Yeast DNA. - [Read Vectorette PCR of Yeast DNA Protocol]

Vectors and Agrobacterium Hosts for Arabidopsis Transformation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.ip29

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

A. tumefaciens is a soil-dwelling bacterium that transforms normal plant cells into tumor-forming cells by inserting a piece of bacterial DNA (the transfer, or "T," DNA) into the plant cell genome. The Ti plasmid also carries many of the transfer functions for mobilizing the T-DNA. This article provides a brief discussion of the principles of T-DNA transformation, including consideration of T-DNA vectors and their hosts. - [Read Vectors and Agrobacterium Hosts for Arabidopsis Transformation Protocol]

Vegetative Growth of Tetrahymena Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4498

Category: Tetrahymena Protozoa Protocols

Protocol describes initiating and maintaining cultures for vegetative growth of Tetrahymena. - [Read Vegetative Growth of Tetrahymena Protocol]

Vegetative Propagation of Alfalfa by Stem Cuttings - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/AlfalfaVegetativePropagationByStemCuttings.pdf

Category: Plant Biology Protocols

Alfalfa is an outcrossing species, cultivars consist of populations rather than individual homozygous or inbred lines. After only two cycles of self-pollination, severe inbreeding depression eliminates selfed individuals from populations. To obtain sufficient plant material of relative genetically uniformity (these plants will still necessarily be heterozygous) for experimental purposes, it may be necessary to propagate a single individual or clone. - [Read Vegetative Propagation of Alfalfa by Stem Cuttings]

VEGF Antibody Staining Protocol - http://www.ihcworld.com/_protocols/antibody_protocols/vegf_vg1_chemicon.htm

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for VEGF antibody staining. Includes: Primary Antibody; Antigen Retrieval; Detection Methods; Chromogen Substrate; Counterstain. - [Read VEGF Antibody Staining Protocol]

Very short introduction to light microscopy and digital imaging PDF - http://www.rpgroup.caltech.edu/courses/aph162/2006/Protocols/microscopy.pdf

Category: Molecular Imaging: Light Microscopy

Very short introduction to light microscopy and digital imaging. Hernan G. Garcia - [Read Very short introduction to light microscopy and digital imaging PDF]

Viability Cell Count - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-7.html

Category: Cell Biology and Cell Culture

Viability Cell Count. Cell Biology Laboratory Manual. Heidcamp - [Read Viability Cell Count]

Viability Cell Count Protocol - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-7.html

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol for the measurement of viability in cell count. - [Read Viability Cell Count Protocol]

Vincenzo Covelli - Guide to the Necropsy of the Mouse - http://www.eulep.org/Necropsy_of_the_Mouse/index.php

Category: Animal Research Techniques

Vincenzo Covelli - Guide to the Necropsy of the Mouse. Rome Italy - [Read Vincenzo Covelli - Guide to the Necropsy of the Mouse]

Viral Detection and Concentration in Food - http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf

Category: Viral Protocols

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods. - [Read Viral Detection and Concentration in Food]

Viral Vector Handling Information - http://www.stanford.edu/dept/EHS/prod/researchlab/bio/docs/Palmer_Bio_protocol.pdf

Category: Cloning Protocols: Vector Protocols

Information on how to handle viral vectors. Includes: Adenovirus; MoMLV retrovirus; MoMLV-based Retroviral Vectors: Base classification BSL-1. - [Read Viral Vector Handling Information]

Virion RNA Dot Blot Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p617

Category: Viral Protocols

Protocol describes the production of a dot blot membrane spotted with RNA isolated from virus particles. - [Read Virion RNA Dot Blot Protocol]

Viscosity & Polymeriztion of Microtubules Protocol - http://homepages.gac.edu/~cellab/chpts/chpt9/ex9-5.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for viscosity and polymerization of microtubules. - [Read Viscosity & Polymeriztion of Microtubules Protocol]

Visualization of RNA Preparations on 1% Agarose Gels Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000025.pdf?pid=PP00000025

Category: Nucleic Acid Electrophoresis Protocols: RNA Electrophoresis Protocols

Method is used to assess (roughly) the integrity of total RNA samples by visualization of discreet 18S and 28S ribosomal RNAs. Total RNA is separated by electrophoresis through a 1% agarose gel containing 1.3 ìM ethidium bromide. Binding of the ethidium bromide to the RNA allows visualization of the separated RNA molecules when the gel is exposed to ultraviolet (UV) light. - [Read Visualization of RNA Preparations on 1% Agarose Gels Protocol]

Visualizing Early Embryo Implantation Sites by Dye Injection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4361

Category: Developmental Biology: Embryology Protocols

This protocol describes a method for visualizing early embryo implantation sites using Chicago Sky Blue 6B dye. Once implantation and interimplantation sites are identified and separated, they can be used for cellular, biochemical, and molecular biology analyses. - [Read Visualizing Early Embryo Implantation Sites by Dye Injection Protocol]

Visualizing miRNAs in Adult Skeletal Muscle by Hybridization In Situ Protocol - http://www.natureprotocols.com/2006/06/29/visualizing_mirnas_in_adult_sk.php

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for visualizing miRNAs in adult skeletal muscle by hybridization in situ. Includes: Visualizing miRNAs in adult skeletal muscle by hybridization in situ; Probe preparation; Tissue preparation; Fixation; Deproteinization with Proteinase K; Acetylation of the tissue; Dehydration; Hybridization step; Post-Hybridization Washes (Step 1-6 should be performed on shaker); Signal Detection (Steps should be performed on a shaker); Post-Detection Washes. - [Read Visualizing miRNAs in Adult Skeletal Muscle by Hybridization In Situ Protocol]

Visualizing miRNAs in Adult Skeletal Muscle by Hybridization in situ Protocol - http://www.natureprotocols.com/2006/06/29/visualizing_mirnas_in_adult_sk.php

Category: Cytogenetics Protocols: RNA In Situ Hybridization Protocols

Vital Staining of Chromosomes with Hoechst 33258 - http://ylwang.umassmed.edu/protocol/cfs/hoechst.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

Vital Staining of Chromosomes with Hoechst 33258. Yu Li Wang labs. - [Read Vital Staining of Chromosomes with Hoechst 33258]

Vitamin Mixtures for Plant Growth Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Vitamin_Mixtures.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for vitamin mixtures for plant growth media. Includes: Preparation of Solutions from Packaged Powder; Media Preparation Using Vitamin Solutions. - [Read Vitamin Mixtures for Plant Growth Media Protocol]

Vogel's Minimal Media Protocol - http://www.fgsc.net/methods/vogels.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Vogel's 50X salts as given in the Microbial Genetics Bulletin 13:42-43, 1956. In 750 ml. distilled water, dissolve successively with stirring at room temperature. - [Read Vogel's Minimal Media Protocol]

Vogels Medium N (Minimal) Protocol - http://www.fgsc.net/methods/vogels.html

Category: Fungi Protocols

Protocol for Vogels medium N (minimal). - [Read Vogels Medium N (Minimal) Protocol]

Watching Molecular Motors at Work by Video-Enhanced Light Microscopy - http://www.mih.unibas.ch/Booklet/Booklet96/Chapter2/Chapter2.html

Category: Microscopy Protocols: Optical Microscopy Protocols

The light microscope allows dynamic biological processes to be imaged in their native (i.e., aqueous) environment with relatively high temporal resolution. However, the diffraction-limited resolution is low. When working at or beyond the diffraction-limited resolution of the LM, a disadvantage of fluorescence imaging is the relatively low signal-to-noise (S/N) ratio of the images. However, this can be increased significantly by video and computer technology. - [Read Watching Molecular Motors at Work by Video-Enhanced Light Microscopy]

Wedding Sands - http://www.weddingsands.com

Category: Affiliates

Wedding information and tips. - [Read Wedding Sands]

Western Analysis Using the Chemiluminescent Alkaline Phosphatase Substrate CSPD Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4256

Category: Western Blot Protocols: Antigen Detection Methods Protocols

Use of the chemiluminescence-producing alkaline phosphatase substrate 3-(4-methoxyspiro[1,2-dioxetane-3,2'-tricyclo-[3.3.1.1(3,7)]decan]-4-yl)phenyl phosphate (AMPPD, also known as adamantyl-1,2-dioxetane phosphate), or its dioxetane relatives provides a substantial increase in sensitivity over colorimetric substrates and radiochemical methods currently used for the detection of antigen-antibody complexes immobilized on nylon or PVDF membranes. - [Read Western Analysis Using the Chemiluminescent Alkaline Phosphatase Substrate CSPD Protocol]

Western Blot - http://www.biochem.emory.edu/labs/acorbe2/protocols9.html

Category: Protein Protocols: Western Blot Protocol

Simple Procedure for Western Blotting. Corbett Lab. - [Read Western Blot]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.