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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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Transient Transfection Into 293T Cells Protocol - http://www.cbrinstitute.org/labs/springer/protocols/jun_293T.html

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1A gene product. 293T is a derivative which also express SV40 large T antigen, allowing episomal replication of plasmids containing the SV40 origin and early promoter region. They (both) have the unusual property of being highly transfectable. - [Read Transient Transfection Into 293T Cells Protocol]

Transient Transfection of COS Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1756

Category: Transfection Protocols: Transient Transfection Protocols

Protocol for the transient transfection of COS cells. - [Read Transient Transfection of COS Cells Protocol]

Transient Transfection of Cos-1 Cells - http://www.upstate.com/misc/protocols.q.prot.e.cDNAprotocol/Transient+Transfection+of+Cos+1+Cells

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Protocol optimized for Cos-1 cells. Successful transfection of each cell type requires optimization of the basic protocol. LIPOFECTAMINE method of transient transfection. Upstate. - [Read Transient Transfection of Cos-1 Cells]

Transient Transfection Protocol: Viral Production - http://genetics.med.harvard.edu/~cepko/protocol/mike/V2.html

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection protocol for the ecotropic/amphotropic producer line including titering and concentration of viral stocks. - [Read Transient Transfection Protocol: Viral Production]

Transient Transfection: Viral Production - http://genetics.med.harvard.edu/~cepko/protocol/mike/V1.html

Category: Transfection Protocols: Transient Transfection Protocols

This transient transfection protocol for the ecotropic/amphotropic producer line includes instructions for titering and concentration of viral stocks. This procedure gives consistently higher transfection efficiency. - [Read Transient Transfection: Viral Production]

Transplantation of Tissues Under the Kidney Capsule Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4382

Category: Mouse Protocols: Mouse Surgical Procedures Protocols

Protocol describes a method for transplanting tissue under the kidney capsule. The in vivo developmental potential of transplanted adult or embryonic tissues can then be determined. - [Read Transplantation of Tissues Under the Kidney Capsule Protocol]

Transposon Mediated Mutagenesis Protocol - http://www.genome.wisc.edu/resources/protocols/tnmutationprotocol.htm

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for transposon mediated mutagenesis. Includes: Amplify ORF from MG1655; Transposition reaction; Transformation; Picking; Verify mutation by Culture PCR; Confirm mutations by sequencing; Curing the temperature sensitive pKD46 plasmid. - [Read Transposon Mediated Mutagenesis Protocol]

Transposon-Mediated Mutagenesis Protocol - http://www.genome.wisc.edu/resources/protocols/tnmutationprotocol.htm

Category: Bacteria Genetics Protocols

Protocol for transposon-mediated mutagenesis. Includes: Amplify ORF from MG1655; Transposition reaction; Transformation; Picking; Verify mutation by Culture PCR; Confirm mutations by sequencing; Curing the temperature sensitive pKD46 plasmid. - [Read Transposon-Mediated Mutagenesis Protocol]

TransWell Chemotaxis Protocol. - http://bioprotocol.bio.com/protocolstools/protocol.jhtml?id=p2156

Category: Immunology: Chemotaxis

TransWell Chemotaxis protocol. trans-well chemotaxis method from bioprotocol. The protocol is a method for studying migration towards a concentration gradient of chemoattractant of leukocytes (neutrophils, monocytes and lymphocytes) or other migratory cells. An upper chamber containing a suspension of cells is separated by a membrane from a lower chamber containing medium with chemoattractant. Chemotaxis of the cells from the upper chamber into the lower chamber can be quantified. - [Read TransWell Chemotaxis Protocol.]

Treat MOUSE EMBRYONIC FIBROBLASTS (MEFs) with Mitomycin C - http://www.uphs.upenn.edu/abramson/simonPlasmids.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: MEF Protocols Mouse Embryonic Fibroblasts

Treat MOUSE EMBRYONIC FIBROBLASTS (MEFs) with Mitomycin C - [Read Treat MOUSE EMBRYONIC FIBROBLASTS (MEFs) with Mitomycin C]

Treatment of Cells with 5-aza-dC. protocol PDF - http://www.shmu.edu.cn/courses/2005aut/upload/20051116/Hongmei%20Xu%202003%20CANCER%20RESEARCH%20%20Aberrant%20Methylation%20and%20Silencing%20of%20ARHI,%20an%20Imprinted%20Tumor%20Suppressor%20Gene%20in%20which%20the%20Function%20Is%20Lost%20in%20Breast%

Category: Epigenetics and Methylation Protocols: 5-Aza-dC Treatment AZA Procedure

"Cells were seeded at a density of 1X106 cells/100-mm dish with 10% FBS and allowed to attach over a 24-h period. 5-Aza-dC (Sigma) was then added to a final concentration of 0.2â€“1 M, and the cells were allowed to grow for 5 days. The medium with or w - [Read Treatment of Cells with 5-aza-dC. protocol PDF]

Tricineâ€“SDS-PAGE Protocol PDF - http://www.nature.com/nprot/journal/v1/n1/pdf/nprot.2006.4.pdf

Category: Protein Protocols: Protein Electrophoresis

Tricineâ€“SDS-PAGE Protocol and background. Nature. PDF file. Tricineâ€“SDS-PAGE is commonly used to separate proteins in the mass range 1â€“100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. â€“SDS-PAGE is also used preferentially for doubled SDS-PAGE (dSDS-PAGE), a proteomic tool used to isolate extremely hydrophobic proteins for mass spectrometric identification. - [Read Tricineâ€“SDS-PAGE Protocol PDF]

Triparental Mating Procedure for Xanthomonas (Recipient) Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/xctripar.html

Category: Bacteria Genetics Protocols

Protocol for triparental mating procedure for Xanthomonas (recipient). - [Read Triparental Mating Procedure for Xanthomonas (Recipient) Protocol]

Triple Color FISH Technique for Mouse Chromosome Identification Protocol - http://info.med.yale.edu/genetics/ward/tavi/mamgenome.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for triple color FISH technique for mouse chromosome identification. - [Read Triple Color FISH Technique for Mouse Chromosome Identification Protocol]

Troubleshoot Immunoprecipitation - http://www.chemicon.com/resource/ANT101/a2.asp#TroubleShooting

Category: Protein Protocols: Immunoprecipitation Protocols: Immunoprecipitation Troubleshooting

Troubleshoot Immunoprecipitation. Chemicon. The most common challenge with immunoprecipitation is trying to lower the number and type of background proteins that contaminate the washed immune complexes. Suggestions for decreasing background in IP. - [Read Troubleshoot Immunoprecipitation]

Troubleshooting Arabidopsis Oligonucleotide Microarrays - http://ag.arizona.edu/microarray/Troubleshooting1.html

Category: DNA Microarray Protocols: Troubleshooting DNA Microarray

Troubleshooting Arabidopsis Oligonucleotide Microarrays, David Galbraith. - [Read Troubleshooting Arabidopsis Oligonucleotide Microarrays]

Troubleshooting for PCR and multiplex PCR - http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

Troubleshooting for PCR and multiplex PCR - http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html

Category: PCR Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

Troubleshooting Guide for Chemiluminescent Western Blotting Pierce - http://www.piercenet.com/Objects/View.cfm?type=Page&ID=0C7FDAA1-EB8C-42AD-90DE-5EA92505449F#trbleshoot

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Troubleshooting Guide for Chemiluminescent Western Blotting Pierce. High Background, Reverse image on film, Blot glows in the darkroom, Signal duration is less than 8 hours, Weak or no signal, Spots within the protein bands, non-specific bands. - [Read Troubleshooting Guide for Chemiluminescent Western Blotting Pierce]

Troubleshooting Guide to Microarray Printing - http://arrayit.com/Products/Printing/Troubleshooting_Guide/troubleshooting_guide.html

Category: DNA Microarray Protocols: Troubleshooting DNA Microarray

Troubleshooting Guide to Microarray Printing. Arrayit. - [Read Troubleshooting Guide to Microarray Printing]

Troubleshooting Guide: Western Blot - http://www.rndsystems.com/tsg_detail_objectname_western_blot.aspx

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Problem: Poor Transfer, Problem: Poor Transfer. R&D Systems. - [Read Troubleshooting Guide: Western Blot]

Troubleshooting Immunoprecipitation - http://www.roche-applied-science.com/PROD_INF/MANUALS/epitope/p66.pdf

Category: Protein Protocols: Immunoprecipitation Protocols

Troubleshooting Immunoprecipitation. Roche. - [Read Troubleshooting Immunoprecipitation]

Troubleshooting Long PCR Roche - http://www.roche-applied-science.com/PROD_INF/MANUALS/pcr_man/Chapter04/CHAP04-Seite76.htm

Category: PCR Protocols: Long PCR Protocol. Extended PCR.

Troubleshooting Long PCR Roche - [Read Troubleshooting Long PCR Roche]

Troubleshooting the immunoprecipitation procedure IP and IB - Roche - http://www.roche-applied-science.com/PROD_INF/MANUALS/epitope/p66.pdf

Category: Protein Protocols: Immunoprecipitation Protocols: Immunoprecipitation Troubleshooting

Troubleshooting the immunoprecipitation procedure Roche. Tagged proteins not visible or only weakly visible on Western blot. High background or many nonspecific bands on gel or blot membrane. - [Read Troubleshooting the immunoprecipitation procedure IP and IB - Roche]

Troubleshooting the Western blot procedure Roche PDF - http://www.roche-applied-science.com/PROD_INF/MANUALS/epitope/p34-35.pdf

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Troubleshooting the Western blot procedure Roche. PDF - [Read Troubleshooting the Western blot procedure Roche PDF]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.