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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Whole-Mount In Situ Hybridization for the Detection of RNA in C. elegans Embryos Protocol - http://cobweb.dartmouth.edu/~ambros/worms/14.html

Category: C. elegans Protocols

Protocol for whole-mount in situ hybridization for the detection of RNA in C. elegans embryos. Includes: Collection of Embryos; Permeabilization and fixation of embryos; Prehybridization; Hybridization; Post-hybridization washes; Probe Detection; Double-labeling; Interpretation. - [Read Whole-Mount In Situ Hybridization for the Detection of RNA in C. elegans Embryos Protocol]

Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4506

Category: RNAi Technology Protocols: RNAi Avian Embryos Protocols

Protocol describes a method for staining nerve fibers in whole-mount preparations of avian embryos using an antibody against the 160-kD subunit of neurofilaments. This allows the comparison of the branching pattern of motor and sensory neurons between control and experimental embryos. This protocol has been successfully applied for embryos at different stages up to about stage 33 (7 days of incubation). - [Read Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos]

Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4506

Category: Avian Bird Protocols

Widefield Microscopy Nikon Upright with Operating Instructions - http://depts.washington.edu/keck/Widefield/equipment.htm

Category: Molecular Imaging

Widefield Microscopy Nikon Upright with Operating Instructions. Keck Imaging Center. - [Read Widefield Microscopy Nikon Upright with Operating Instructions]

Working with Bacterial Artificial Chromosomes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4010

Category: BACs Bacterial Artificial Chromosome Protocols

Protocol describes how to transform E. coli with BAC DNA by electroporation. - [Read Working with Bacterial Artificial Chromosomes Protocol]

Working with Bacteriophage P1 and Its Cloning Systems Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4003

Category: Viral Manipulation Protocols: Phage Protocols

Protocol describes methods for recovery and purification of recombinant clones of bacteriophage P1 or PAC DNAs from bacteria. Because of their large size, these DNAs are sensitive to shearing forces and must be handled carefully. This protocol generally yields P1 DNA that works well as a substrate or template in enzymatic reactions. - [Read Working with Bacteriophage P1 and Its Cloning Systems Protocol]

Working with Yeast Artificial Chromosomes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3297

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol outlines a procedure to validate and store yeast strains carrying YACs. - [Read Working with Yeast Artificial Chromosomes Protocol]

Worthington Tissue Dissociation Guide - http://www.tissuedissociation.com/techniques.html

Category: Cell Culture Protocols

Includes information on: Cell Isolation Theory ( Tissue Types: Epithelial Tissue, Connective Tissue); Dissociating Enzymes (Collagenase, Trypsin, Elastase, Hyaluronidase, Papain, Chymotrypsin, Deoxyribonuclease I, Neutral Protease (Dispase), Trypsin Inhibitor (Soybean)); Cell Isolation Techniques: Working With Enzymes, Basic Primary Cell Isolation, Equilibration with 95%O2:5%CO2, Trituration, Enzymatic Cell Harvesting, Cell Adhesion and Harvesting, Trypsin for Cell Harvesting, etc... - [Read Worthington Tissue Dissociation Guide]

X-Gal Staining of Drosophila Adult Head Whole Mounts Protocol - http://vosshall.rockefeller.edu/protocols/X-galWholeMount.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for X-gal staining of Drosophila adult head whole mounts. - [Read X-Gal Staining of Drosophila Adult Head Whole Mounts Protocol]

X-Gal Staining of Drosophila Frozen Cryostat Sections Protocol - http://vosshall.rockefeller.edu/protocols/X-galstainingprotocol.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for X-gal staining of Drosophila frozen cryostat sections. - [Read X-Gal Staining of Drosophila Frozen Cryostat Sections Protocol]

X-gal Staining of Wholemount Drosophila Embryos Protocol - http://jfly.iam.u-tokyo.ac.jp/html/manuals/pdf/E_Staining_Uemura.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for X-gal staining of wholemount Drosophila embryos. - [Read X-gal Staining of Wholemount Drosophila Embryos Protocol]

X-Ray Mutagenesis of Drosophila Protocol - http://www.bioprotocol.com/protocolstools/protocol.jhtml%3Bjsessionid%3DGYZOPNVU3IQQTR3FQLMSFEWHUWBNQIV0?id=p32

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol describes the general procedure for creating mutations in the DNA of Drosophila by exposure to X-rays. Irradiation of cells with X-rays creates double strand breaks (DSBs) in DNA. Mutations introduced in the DNA of germ line cells (sperm) are propagated by mating the exposed males to virgin females. The progeny of this cross can be mated to each other so that a percentage of the subsequent offspring will have two copies of the same mutant allele. - [Read X-Ray Mutagenesis of Drosophila Protocol]

Xenopus cDNA Libraries - http://www.xenbase.org/other/static/methods/cDNA_libraries.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Xenopus cDNA libraries. Includes: General cDNA clone providers. - [Read Xenopus cDNA Libraries]

Xenopus Chromatin immmunoprecipitation Protocol - http://spot.colorado.edu/~klym/Methods/XChIP.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus chromatin immunoprecipitation. - [Read Xenopus Chromatin immmunoprecipitation Protocol]

Xenopus DNA Fishing Protocol - http://spot.colorado.edu/~klym/Methods/fishing.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus DNA fishing. Includes: DNA-agarose coupling reaction; PROTEIN BINDING REACTION; OLIGO ANNEALING. - [Read Xenopus DNA Fishing Protocol]

Xenopus Egg CSF Extract Preparation Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1049

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol describes the preparation of Cytostatic Factor (CSF) from frog eggs. CSF can be used in in vitro spindle assembly assays. Includes: Frog injections; Preparation Before Starting the Extract Preparation; Extract Preparation. - [Read Xenopus Egg CSF Extract Preparation Protocol]

Xenopus Fluorescent in situs and FCIS Protocol - http://www.xenbase.org/other/static/methods/FISH.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for performing wholemount fluorescent in situs. Instructions for two-color FISH and for a combination of fluorescent and colorimetric in situs we have called FCIS. Data can be imaged with a fluorescence stereoscope or a confocal. - [Read Xenopus Fluorescent in situs and FCIS Protocol]

Xenopus Gynogenesis Protocol - http://tropicalis.berkeley.edu/home/genetic_techniques/gynogenesis/gynogenesis.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus gynogenesis. - [Read Xenopus Gynogenesis Protocol]

Xenopus Microinjection Protocol - http://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/inject.html

Category: Xenopus Protocols: Xenopus Injection Protocols

Protocol for Xenopus microinjection. - [Read Xenopus Microinjection Protocol]

Xenopus Oocyte Transfer Protocol - http://www.xenbase.org/other/static/methods/anti-methods/as-proto.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus oocyte transfer. - [Read Xenopus Oocyte Transfer Protocol]

Xenopus RNA Isolation Protocol - http://spot.colorado.edu/~klym/Methods/RNAisolation.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus RNA isolation. - [Read Xenopus RNA Isolation Protocol]

Xenopus Sperm Nuclei Preparation Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1050

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol for Xenopus sperm nuclei preparation. Sperm Nuclei can be made and stored as a stock for later use with Cytostatic Factor Extracts. - [Read Xenopus Sperm Nuclei Preparation Protocol]

Xenopus Whole Mount In Situ Hybridization Protocols - http://tropicalis.berkeley.edu/home/gene_expression/in-situ/insitu.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for Xenopus whole mount in situ hybridization. Includes: Protocols for Making Antisense RNA probe (dig labelled); Protocols for In Situ Hyb. - [Read Xenopus Whole Mount In Situ Hybridization Protocols]

YAC Transfer by KAR1Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/YEAST/YACTransfer.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for YAC transfer by KAR1. Includes: YAC transfer by Kar1 mating; Transfer YAC from donor cells into YPH 925; Transfer YAC from YPH925 into recipient cells. - [Read YAC Transfer by KAR1Protocol]

YC Dropout Plate Supplementation Table - http://www.fhcrc.org/science/labs/gottschling/yeast/ycsup.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

YC Dropout Plate Supplementation Table. - [Read YC Dropout Plate Supplementation Table]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.