User Panel

My Panel

Bookmark Science Articles

day Protocols

Search results for: day

Protocols » Search Results

Featured Search Results Links

Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Tetrad Dissection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4181

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

This protocol describes dissection of yeast tetrads. Tetrad analysis is useful for linkage studies and for constructing strains for genetic and biochemical experiments. - [Read Tetrad Dissection Protocol]

Tetrahymena Thermophila Ocular Irritancy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/22.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

The basis of this test is that a cytotoxic chemical (regardless of site or mechanism of action) will interfere with the normal motility of the protozoan, Tetrahymena thermophila, in culture. The degree of interference of motility as compared to control cultures, related to the concentration of the test compound, provides an indication of toxicity. - [Read Tetrahymena Thermophila Ocular Irritancy Test]

TEV Purification Protocol - http://openwetware.org/wiki/Keating:Experimental_Protocols:TEV_purification

Category: Viral Protocols

Protocol for TEV purification. - [Read TEV Purification Protocol]

Thaw Procedure for RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000160.pdf?pid=PP00000160

Category: Cell Culture Protocols: Cell Lines Protocols

Protocol for the thaw procedure for RAW 264.7 cells. Includes: Thaw procedure and reagents and materials. - [Read Thaw Procedure for RAW 264.7 Cells]

Thawing & Freezing Protocols for Insect Cells (BV - Baculovirus ) - eBioscience - http://www.ebioscience.com/ebioscience/appls/BVC.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Thawing & Freezing Protocols for Insect Cells (BV - for Baculovirus ) - eBioscience. Freezing Protocol and Thawing Protocols included. - [Read Thawing & Freezing Protocols for Insect Cells (BV - Baculovirus ) - eBioscience]

Thawing & Freezing Protocols for Insect Cells (BV) - http://www.ebioscience.com/ebioscience/appls/BVC.htm

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

Protocols for thawing and freezing insect cells (BV). - [Read Thawing & Freezing Protocols for Insect Cells (BV)]

Thawing & Freezing Protocols for Insect Cells (BV) - http://www.ebioscience.com/ebioscience/appls/BVC.htm

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Protocols for thawing and freezing insect cells (BV). - [Read Thawing & Freezing Protocols for Insect Cells (BV)]

Thawing Human ES Cells Protocol - http://ink.primate.wisc.edu/~thomson/pdfs/thawing_es.pdf

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Protocol for thawing human ES cells. - [Read Thawing Human ES Cells Protocol]

The Basic Polymerase Chain Reaction Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3824

Category: PCR Protocols: Standard PCR Protocol

Protocol describes how to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction. - [Read The Basic Polymerase Chain Reaction Protocol]

The Cell Attached Capacitance Recording Technique - http://www.natureprotocols.com/2006/11/03/cell_attached_capacitance_meas.php

Category: Neuroscience Protocols: Patch Clamping Protocols

The cell-attached capacitance recording technique is a powerful technique that has been successfully used to resolve single vesicle fusion and fusion pore conductance. This technique, however, has not been applied to synapses because of the difficulty in accessing release sites. Here, we developed a technique to expose release sites in a large nerve terminal, the calyx of Held, which contains clear-core glutamatergic vesicles. - [Read The Cell Attached Capacitance Recording Technique]

The Cell Growth Environment - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook/Cell_Culture_Techniques_6.html#Environment

Category: Cell Biology and Cell Culture: Cell Culture Growth Media

Cultured cells require a sterile environment and a supply of nutrients for growth. Basic Constituents of media. Sigma - [Read The Cell Growth Environment]

The Frame Cytotoxicity Test Kenacid Blue - http://embryo.ib.amwaw.edu.pl/invittox/prot/15.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

The cytotoxic effect of chemicals upon cells in culture is measured by the change in total cell protein arising from the inhibition of cell proliferation (Kenacid Blue R dye binding method). - [Read The Frame Cytotoxicity Test Kenacid Blue]

The Hill Reaction Protocol - http://homepages.gac.edu/~cellab/chpts/chpt8/ex8-3.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol on how to calculate the hill reaction. - [Read The Hill Reaction Protocol]

The Isolation and Culture of Rat Hepatic Cells Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/7.htm

Category: Primary Cell Isolation and Culture Protocols

This method enables the isolation of different populations of liver cells and describes their subsequent culture. - [Read The Isolation and Culture of Rat Hepatic Cells Protocol]

The Isolation and Culture of Rat Hepatic Cells Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/7.htm

Category: Mouse Protocols: Mouse Cell Culture Protocols

The liver of a rat is cannulated and perfused in situ with buffer, following which it is excised and perfused in a closed system with a collagenase solution. After a period of time the liver begins to break up, at which point it is transferred to a measuring cylinder and culture medium is added. It is then gently agitated to cause the release of cells which are subsequently filtered and allowed to settle out. The parenchymal and non-parenchymal cells form two distinct layers which can be separat - [Read The Isolation and Culture of Rat Hepatic Cells Protocol]

The Isolation and Culture of Rat Hepatic Cells Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/7.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Liver Toxicity Assays Protocols

This method enables the isolation of different populations of liver cells and describes their subsequent culture. - [Read The Isolation and Culture of Rat Hepatic Cells Protocol]

The Liquid Scintillation Counting Process - http://laxmi.nuc.ucla.edu:8248/M248_99/autorad/Scint/scint1.html

Category: Radioactivity: Liquid Scintillation Counting

The Liquid Scintillation Counting Process. Great Review of the Process and detection procedure of the counter. Dr. Gambhir - [Read The Liquid Scintillation Counting Process]

The Patch Clamp Technique - http://www.cellsalive.com/patch.htm

Category: Electrophysiology Ion Channels

The Patch Clamp Technique. Nice pictures of the technique. Cells Alive - [Read The Patch Clamp Technique]

The Patch Clamp Technique - http://www.bio.psu.edu/People/Faculty/Assmann/Lab/techniques.html

Category: Neuroscience Protocols: Patch Clamping Protocols

Learn about the patch clamp technique. - [Read The Patch Clamp Technique]

The Procedure for Optimized ELISA Protocol - http://www.pall.com/34445_4769.asp

Category: ELISA Protocols

The Procedure for Optimized ELISA Protocol. PALL Corp. - [Read The Procedure for Optimized ELISA Protocol]

The siRNA user guide - http://www.rockefeller.edu/labheads/tuschl/sirna.html

Category: siRNA and RNAi Protocols

Selection of siRNA duplexes from the target mRNA sequence, Preparation of siRNA duplexes, Useful siRNA reagent combinations, Transfection of siRNA duplexes, Sequences of siRNA duplexes used in our studies. Tuschl Lab. - [Read The siRNA user guide]

The siRNA User Guide - http://www.rockefeller.edu/labheads/tuschl/sirna.html

Category: RNAi Technology Protocols

The siRNA user guide. Includes: Selection of siRNA duplexes from the target mRNA sequence; Preparation of siRNA duplexes; Useful siRNA reagent combinations; Transfection of siRNA duplexes; Sequences of siRNA duplexes used in our studies. - [Read The siRNA User Guide]

The Transmission Electron Microscope - http://homepages.gac.edu/~cellab/chpts/chpt1/ex1-11.html

Category: Microscopy Protocols: Electron Microscopy Protocols

Protocol on how to use the transmission electron microscope. - [Read The Transmission Electron Microscope]

The Under-Agarose Migration Assay - http://bioprotocol.bio.com/protocolstools/protocol.jhtml?id=p2157

Category: Signalling Signal Transduction Protocols: Chemokine Protocols

Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. BioProtocol - [Read The Under-Agarose Migration Assay]

The Use of Flow Cytometry for Concomitant Detection of Apoptosis and Cell Cycle Analysis - http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.2_96/pp25-27.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Two distinct modes of cell death, apoptosis and necrosis, can be distinguished on the basis of differences in morphological, biochemical, and molecular changes occurring in the dying cells. Report the development of flow cytometric techniques that permit concomitant detection of apoptosis and cellular DNA content or BrdU content analysis. - [Read The Use of Flow Cytometry for Concomitant Detection of Apoptosis and Cell Cycle Analysis]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.