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Affinity purification of antibodies from crude serum - http://wheat.pw.usda.gov/~lazo/methods/au/sect32.html

Category: Protein Protocols: Antibody Purification Protocols

Affinity purification of antibodies from crude serum. David Bowtell Lab. - [Read Affinity purification of antibodies from crude serum]

Analysis of DNA by Restriction Enzyme Cleavage and Agarose Gel Electrophoresis - http://www.ambl.msl.ubc.ca/exp2.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Very detailed class protocol for Analysis of DNA by Restriction Enzyme Cleavage and Agarose Gel Electrophoresis. David Ng, UBC. - [Read Analysis of DNA by Restriction Enzyme Cleavage and Agarose Gel Electrophoresis]

Analysis of Protein Gels (SDS-PAGE) - http://www.ruf.rice.edu/~bioslabs/studies/sds-page/gellab3.html

Category: Protein Protocols: Protein Electrophoresis

An excellent guide on the analysis of proteins on SDS-PAGE gels, through staining with coomassie blue dye and western blot analysis. Analysis of Protein Gels (SDS-PAGE). David R. Caprette, Rice University. - [Read Analysis of Protein Gels (SDS-PAGE)]

Chemotaxis Under Agar Assay - http://dictybase.org/techniques/chemotaxis/Knecht_UnderagarAssay.pdf

Category: Immunology: Chemotaxis

Chemotaxis Under Agar Assay by David Knecht. - [Read Chemotaxis Under Agar Assay]

Detection and Measurement of Radioactivity - http://www.ruf.rice.edu/%7Ebioslabs/methods/radioisotopes/rad1.html

Category: Radioactivity

Radioactive Decay, Methods of detection, Liquid scintillation counting, Background and Quenching, The scintillation counter printout. David R. Caprette, Rice Univ. - [Read Detection and Measurement of Radioactivity]

Introduction to SDS-PAGE - http://www.ruf.rice.edu/%7Ebioslabs/studies/sds-page/gellab2.html#gelprep

Category: Protein Protocols: Protein Electrophoresis

A great introduction to SDS-PAGE. David R. Caprette, Rice University. - [Read Introduction to SDS-PAGE]

Light Microscopy - http://www.ruf.rice.edu/%7Ebioslabs/methods/microscopy/microscopy.html

Category: Molecular Imaging: Light Microscopy

Types of light microscopy, Bright Field Microscopy, Using a bright field microscope, mounting on slides, adjusting the microscope, Care of the microscope, When to use bright field microscopy. David R. Caprette. Rice University. - [Read Light Microscopy]

Mini-Prep Protocol for Purification of Plasmid DNA from Bacteria - http://people.morehead-st.edu/fs/d.peyton/protocols.html

Category: Microbiology: Mini-Prep Plasmid Purification

Using Solutions A for Lysis, Solutions B for Neutralization Solution, and Solution C for washing. Dr.David Peyton. - [Read Mini-Prep Protocol for Purification of Plasmid DNA from Bacteria]

SDS-PAGE Gels - http://mycoplasmas.vm.iastate.edu/lab_site/methods/protein/SDS-PAGE.html

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS-PAGE Gels. Adapted from David Bowtell - [Read SDS-PAGE Gels]

Selection of Poly(A)+ RNA by Batch Chromatography - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4048

Category: Chromatography Protocols: DNA RNA Affinity Chromatography

When many RNA samples are to be processed or when working with small amounts (<50 Âµg) of total mammalian RNA, the technique of choice is batch chromatography on oligo(dT)-cellulose. The method described in this protocol uses a combination of temperature and ionic strength to maximize binding and recovery of polyadenylated RNA. IMPORTANT: Prepare all reagents used in this protocol with Diethyl pyrocarbonate (DEPC)-treated H2O. Joseph Sambrook and David W. Russell. - [Read Selection of Poly(A)+ RNA by Batch Chromatography - Subscription Required]

Troubleshooting Arabidopsis Oligonucleotide Microarrays - http://ag.arizona.edu/microarray/Troubleshooting1.html

Category: DNA Microarray Protocols: Troubleshooting DNA Microarray

Troubleshooting Arabidopsis Oligonucleotide Microarrays, David Galbraith. - [Read Troubleshooting Arabidopsis Oligonucleotide Microarrays]

Yeast and Bacterial Colony PCR Protocol. - http://www.upstate.edu/biochem/amberg/protocols/colony_pcr.html

Category: PCR Protocols: Colony PCR

Yeast and Bacterial Colony PCR Protocol. David Amberg SUNY Upstate Medical University - [Read Yeast and Bacterial Colony PCR Protocol.]

Yeast Genomic DNA Isolation Procedure - http://www.upstate.edu/biochem/amberg/protocols/yeast_genomic_DNA.html

Category: DNA Protocols: DNA Extraction Protocols

Yeast Genomic DNA Isolation David Amberg, Upstate Medical University. - [Read Yeast Genomic DNA Isolation Procedure]