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Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4523

Category: C. elegans Protocols: C. elegans Staining Protocols

Extreme care should be used to identify and verify positive reactions, however, because cross-reactions are common. Counterstaining is essential for examining worms by immunofluorescence and is used to identify the exact cell in which an antigen appears. Methods for counterstaining include labeling all cells with a fluorescent dye that is specific for nucleic acids (e.g., DAPI or propidium iodide) and using GFP driven by tissue-specific promoters. - [Read Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol]

COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL - http://users.ugent.be/~fspelema/neubla/protocol/cgh.htm

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

SAVING OF DAPI-IMAGES, SLIDE PRETREATMENT, PROBE PREPARATION, PROBE DETECTION, washing blocking detection, Counterstaining with DAPI. Institute of Pathology,Humboldt-University of Berlin - [Read COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL]

DAPI Staining Protocol - http://www.genetics.wustl.edu/tslab/Protocols/dapi_staining.html

Category: C. elegans Protocols: C. elegans Staining Protocols

Protocol for DAPI staining. - [Read DAPI Staining Protocol]

DAPI Staining Yeast Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/dapistain.html

Category: Yeast Protocols: Yeast Staining Protocols

Protocol for DAPI staining yeast. - [Read DAPI Staining Yeast Protocol]

Determining Cell Cycle Stages by Flow Cytometry Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662F660A11AB3ABD97E38B90B2AF0D&objectid=6674031CC5AE0F442766156EA393FE81

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Describes assays used to determine the distribution of a population of cells to the different stages of the cell cycle as analyzed by flow cytometry. Staining the DNA with different fluorescent dyes, propidium iodide or DAPI, is one of the most direct ways of staging the cells based on DNA content. - [Read Determining Cell Cycle Stages by Flow Cytometry Protocol]

DNA laddering assay for treated cells - http://www.celldeath.de/apometh/dnaladd.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols

DNA laddering can be detected from samples with only 8% apoptotic cells. Alternatively, the cells can be stained with DAPI and analysed by flow cytometry. CellDeath.de - [Read DNA laddering assay for treated cells]

NTS Buffer DAPI Stain Protocol - http://depts.washington.edu/immunweb/cellanalysis/DAPI.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

Protocol for NTS Buffer DAPI Stain - [Read NTS Buffer DAPI Stain Protocol]

Propidium Iodide (PI) or DAPI Staining of Unfixed Solid Tissues for Flow Cytometry Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4438

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

This protocol describes a method for quantitative measurement of DNA in solid tissue samples using either propidium iodide (PI) or DAPI staining followed by flow cytometry. PI can be excited at 488 nm by the argon-ion laser, the most commonly used laser in flow cytometry. Alternatively, DAPI is best excited by a high-power UV laser, which is less commonly available. - [Read Propidium Iodide (PI) or DAPI Staining of Unfixed Solid Tissues for Flow Cytometry Protocol]

Propidium Iodide (PI) or DAPI Staining of Unfixed Tissue Culture Cells for Flow Cytometry Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4437

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

This protocol describes a method for quantitative measurement of DNA in tissue culture cells using either propidium iodide (PI) or DAPI staining followed by flow cytometry. PI can be excited at 488 nm by the argon-ion laser, the most commonly used laser in flow cytometry. Alternatively, DAPI is best excited by a high-power UV laser, which is less commonly available. - [Read Propidium Iodide (PI) or DAPI Staining of Unfixed Tissue Culture Cells for Flow Cytometry Protocol]

Protocol to Count Cell Number of Preimplantation Embryos - http://www.animal.ufl.edu/hansen/protocols/embryocellnumber.htm

Category: Developmental Biology: Embryology Protocols

Protocol to Count Cell Number of Preimplantation Embryos using Nuclear Staining with Hoechst 33342 or DAPI. Includes: Preparation of Embryos; Preparation of Hoechst 33342 dye; Preparation of DAPI; Staining the Embryo; Mounting Embryos to Slides; What to Do When There are Too Many Cells to Count. - [Read Protocol to Count Cell Number of Preimplantation Embryos]

Rapid DAPI Screen for Chromosome Missegregation Mutants Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/missegscreen.html

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol for a rapid screen for chromosome missegregation mutants by DAPI staining. - [Read Rapid DAPI Screen for Chromosome Missegregation Mutants Protocol]

Whole-Mount DAPI Staining and Measurement of DNA Content in Plant Cells - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4684

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

During development many plant cells undergo endoreduplication, whereby ploidy increases to a multiple of the normal 2C content. For eg., trichome development is accompanied by an increase in ploidy to 32C, indicating that trichome cells undergo four rounds of endoreduplication. Protocol describes DNA levels, and hence developmental progress in the corresponding cells, are measured by staining the DNA with a fluorescent marker and then quantifying the fluorescence of individual nuclei. - [Read Whole-Mount DAPI Staining and Measurement of DNA Content in Plant Cells]

Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4163

Category: Yeast Protocols: Yeast Staining Protocols

Protocol describes the staining of nuclear and mitochondrial DNA with DAPI (4',6-diamidino-2-phenylindole). - [Read Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol]