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Calibration of Micropipette Tips Protocols - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4655

Category: Microinjection Protocols: Microinjection Equipment

This protocol describes an easy method for calibrating micropipette tips that have been pulled in the laboratory. It is essential to estimate the internal diameter of the pulled micropipette tip when adjusting parameters for a new puller or new type of glass tubing. A tip diameter of ~0.3 µm is optimal for the microinjection of mammalian cells in culture (e.g., CHO, PtK1, and COS-7). A 10% increase in diameter increases the delivery rate by more than 30% and can cause cell damage. - [Read Calibration of Micropipette Tips Protocols]

Immunostaining of DDR Proteins in Senescent Cells Protocol - http://www.natureprotocols.com/2006/12/06/immunostaining_of_ddr_proteins_1.php

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Protocol for immunostaining of DDR proteins in senescent cells. Detailed methodology to study the activation of a number of DNA damage response factors in senescent human fibroblasts. - [Read Immunostaining of DDR Proteins in Senescent Cells Protocol]

Indirect Immunofluorescence with Preextraction (In Situ Cell Fractionation) Protocol - http://www.natureprotocols.com/2006/12/05/indirect_immunofluorescence_wi.php

Category: Immunology: Immunofluorescence Protocols

The study of the activation of DNA damage response factors at the single cell level relies on the observation of their accumulation (or the accumulation of their activated forms) in nuclear foci by plain immunofluorescence techniques. - [Read Indirect Immunofluorescence with Preextraction (In Situ Cell Fractionation) Protocol]

Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4393

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol describes a method for purification of YAC DNA using ultrafiltration. This is the fastest method available, but care needs to be taken not to damage the DNA. The purified DNA can then be used for microinjection. - [Read Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units Protocol]

V79 Cytotoxicity Test for Membrane Damage - http://embryo.ib.amwaw.edu.pl/invittox/prot/39.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

The cytotoxic effect of test chemicals in V79 cell culture can be determined by assessing damage to the plasma membrane as determined by a nucleic acid leakage assay. - [Read V79 Cytotoxicity Test for Membrane Damage]