cytosolic Protocols

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A Self-generated Gradient to Discriminate a Cytosolic or Membrane Location of Large Protein Complex - http://www.axis-shield.com/densityhome/optiprep/S24.pdf

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols

Protocol describes systems in which the membranes are allowed to float through a density gradient from a dense load zone. This is an ideal format. - [Read A Self-generated Gradient to Discriminate a Cytosolic or Membrane Location of Large Protein Complex]

Cytosolic Extract & Immunoprecipitation Protocol - http://www.genetics.wustl.edu/tslab/Protocols/CytosolicExtractv1_v2.htm

Category: C. elegans Protocols

Protocol for cytosolic extract & immunoprecipitation. Includes: Culture; Harvest; Extract; Immunoprecipitation. - [Read Cytosolic Extract & Immunoprecipitation Protocol]

Isolation of Genomic and Viral DNA from Lymphocytes Protocol - http://www1.qiagen.com/literature/protocols/pdf/QG03.pdf

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for the isolation of genomic and viral DNA from lymphocytes. Includes: Viral DNA in cytosolic fraction and Viral DNA in nuclear fraction integrated. - [Read Isolation of Genomic and Viral DNA from Lymphocytes Protocol]

Nuclear and Splicing Extract Preparation - http://penguin.uchc.edu/~intron/Protocols/NuclearS100%20Extract.html

Category: Protein Protocols: Nuclear Extraction Protocols

S100 extract cytosolic extraction preparation. Buffer A (Hypotonic Buffer), Low and High salt buffer recipes. Brent Graveley - [Read Nuclear and Splicing Extract Preparation]

Preparation of Worm Extracts by Sonication Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Cyto_extract_sonicat_v2.htm

Category: C. elegans Protocols

Protocol was designed to rapidly generate small scale cytosolic extracts of C. elegans for Western or IP (has not been tested for RNA work). The protocol works well for between 50 to 5000 worms and has not been extensively tested on larger a scale, though it should work. Includes: Collection; Sonication; Clearing lysate; Immunoprecipitation. - [Read Preparation of Worm Extracts by Sonication Protocol]