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Propidium Iodide Staining of Dead Cells for Flow Cytometry Protocol - http://cyto.mednet.ucla.edu/Protocols/pi.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Propidium iodide (PI) intercalates into double-stranded nucleic acids. It is excluded by viable cells but can penetrate cell membranes of dying or dead cells. - [Read Propidium Iodide Staining of Dead Cells for Flow Cytometry Protocol]

Protocol for Isolation, Cryopreservation, and Thawing of PBMCs - http://www.biomedcentral.com/content/supplementary/1471-2172-6-17-S1.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Cryopreserved PBMCs are a common specimen source for studies of immunological responses to vaccines, immunotherapies, etc. The health and viability of cells recovered post-cryopreservation are of course critical to the success and accuracy of immunological assays performed on them. This protocol standardizes PBMC isolation and cryopreservation techniques, specifically for the assessment of thawed cells by cytokine flow cytometry. - [Read Protocol for Isolation, Cryopreservation, and Thawing of PBMCs]

Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio - http://www.fhcrc.org/science/labs/fero/Protocols/Hematopoietic.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Antibodies for Lineage Depletion; Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining. - [Read Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio]

Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/Hematopoietic.pdf

Category: Immunology

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining. - [Read Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol]

Quantitative Flow Cytometry for Apoptosis - http://www.biotech.uiuc.edu/centers/Proteomics/Flow_cytometry/docs/Apopbroc.pdf

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Quantitative Flow Cytometry for Apoptosis Detection. Flow Cytometry Facility, U.Illinois. - [Read Quantitative Flow Cytometry for Apoptosis]

Rat Hepatocyte Flow Cytometric Cytotoxicitiy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/23.htm#TOP

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Liver Toxicity Assays Protocols

Flow cytometry is used to monitor drug-induced changes in DNA and protein contents of hepatocytes cultured at physiological oxygen concentrations. - [Read Rat Hepatocyte Flow Cytometric Cytotoxicitiy Test]

Separation of Platelets from Whole Blood - http://www.cbrinstitute.org/labs/springer/protocols/azucena_platelets.html

Category: Cell Biology and Cell Culture

This protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamber assays, flow cytometry measurements, etc. - [Read Separation of Platelets from Whole Blood]

Sodium and Potassium Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/sodium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for sodium and potassium analysis by flow cytometry. Includes: Preparation of the dyes; Loading of the dyes; Flow Cytometric Analysis. - [Read Sodium and Potassium Analysis by Flow Cytometry Protocol]

Sodium and Potassium Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/sodium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

The Use of Flow Cytometry for Concomitant Detection of Apoptosis and Cell Cycle Analysis - http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.2_96/pp25-27.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Two distinct modes of cell death, apoptosis and necrosis, can be distinguished on the basis of differences in morphological, biochemical, and molecular changes occurring in the dying cells. Report the development of flow cytometric techniques that permit concomitant detection of apoptosis and cellular DNA content or BrdU content analysis. - [Read The Use of Flow Cytometry for Concomitant Detection of Apoptosis and Cell Cycle Analysis]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Protocol for yeast cell cycle by flow cytometry. Includes procedure for dyes: Propidium Iodide and Sytox Green. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Protocol for yeast cell cycle by flow cytometry. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Category: Yeast Protocols

Protocol for Yeast cell cycle by flow cytometry. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

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