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Additional slide pre-treatment for mapping smaller insert clones - http://www.sanger.ac.uk/HGP/methods/cytogenetics/additional_treatment.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Additional slide pre-treatment for mapping smaller insert clones for FISH. Sanger Institute. - [Read Additional slide pre-treatment for mapping smaller insert clones]

Applications of SKY in Cancer Cytogenetics - http://www.utoronto.ca/cancyto/protocols_software/cytogenetics/SKY.html

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

SKY has been applied to various tumor groups including hematological malignancies, sarcomas, carcinomas and brain tumors, with the intent of identifying specific chromosomal abnormalities that may provide insight to the genes involved in the disease process as well as identifying recurrent cytogenetic markers for clinical diagnosis and prognostic assessment. - [Read Applications of SKY in Cancer Cytogenetics]

Applications of SKY in Cancer Cytogenetics Review - http://www.utoronto.ca/cancyto/protocols_software/cytogenetics/SKY.html

Category: Genetics and Genomics: Cancer Genetics Protocols

SKY has also been applied for the mouse genome, enabling investigators to extrapolate information from mouse models of cancer to their human counterparts. This review will address the advances that SKY has facilitated in the field of cancer cytogenetics, as well as its variety of application in the cancer research laboratories. - [Read Applications of SKY in Cancer Cytogenetics Review]

Bacterial clone DNA Protocol for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/DNAprep.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Bacterial clone DNA Protocol for FISH. - [Read Bacterial clone DNA Protocol for FISH]

CGH array hybridisation Protocol - http://www.sanger.ac.uk/HGP/methods/cytogenetics/Hyb.shtml

Category: Cytogenetics Protocols: Array CGH

Detailed CGH array hybridisation (gridsize: 2 x 1.5 cm). Sanger Institute. - [Read CGH array hybridisation Protocol]

DNA FIBERS FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/fiber_fish.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

DNA FIBERS FISH Protocol and Methods. Sanger Institute. - [Read DNA FIBERS FISH]

DOP PCR For CGH Array - http://www.sanger.ac.uk/HGP/methods/cytogenetics/DOPPCR.shtml

Category: Cytogenetics Protocols: Array CGH

DOP PCR Protocol For CGH Array. - [Read DOP PCR For CGH Array]

FISH Hybridization Protocol - http://www.sanger.ac.uk/HGP/methods/cytogenetics/hybridisation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Hybridization Protocol. Sanger Institute. - [Read FISH Hybridization Protocol]

Hybridisation of Interphase Nuclei Protocol - http://www.sanger.ac.uk/HGP/methods/cytogenetics/interphase_hybridisation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Hybridisation of Interphase Nuclei Method. Sanger Institute. - [Read Hybridisation of Interphase Nuclei Protocol]

Interphase nuclei - http://www.sanger.ac.uk/HGP/methods/cytogenetics/interphase_nuclei.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Interphase nuclei preparation for FISH. Sanger Institute. - [Read Interphase nuclei]

Molecular and Genetic Toxicology Studies for In Vitro Cytogenetics with CHO or human lymphocytes - http://www.sri.com/biosciences/bdd/toxic/protocol.html#7

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Molecular and genetic toxicology studies for in vitro cytogenetics with CHO or human lymphocytes. Includes: G401 Chromosomal Aberrations in Chinese Hamster Ovary (CHO) Cells, G401-2, G401-R, G401-R2, G401-R2H, G402 Chromosomal Aberrations in Human Lymphocytes, G402-2, G402-R, G402-R2, G402-R2H, G403 Sister Chromatid Exchanges in Chinese Hamster Ovary (CHO) Cells. - [Read Molecular and Genetic Toxicology Studies for In Vitro Cytogenetics with CHO or human lymphocytes]

Molecular and Genetic Toxicology Studies for In Vivo Cytogenetics - http://www.sri.com/biosciences/bdd/toxic/protocol.html#8

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Molecular and genetic toxicology studies for in vivo cytogenetics. Includes: G501 Chromosomal Aberrations in Rodent Bone Marrow Cells, G502 Single-Exposure Bone Marrow Cytotoxicity Assay. - [Read Molecular and Genetic Toxicology Studies for In Vivo Cytogenetics]

Nick translation for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/nick_translation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Nick translation for FISH. Sanger Institute. - [Read Nick translation for FISH]

Preparation of metaphase chromosomes from lymphoblastoid cell lines - http://www.sanger.ac.uk/HGP/methods/cytogenetics/metaphase.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Preparation of metaphase chromosomes from lymphoblastoid cell lines for FISH analysis. Sanger Institue. - [Read Preparation of metaphase chromosomes from lymphoblastoid cell lines]

Random Labelling DNA for array CGH Protocol - http://www.sanger.ac.uk/HGP/methods/cytogenetics/Labelling.shtml

Category: Cytogenetics Protocols: Array CGH

Random Labelling DNA for array CGH Protocol. Sanger Institute - [Read Random Labelling DNA for array CGH Protocol]

Silver Staining for Nucleolar Organizer Regions - http://www.le.ac.uk/biology/phh4/agnor.htm

Category: Cytogenetics Protocols

Silver Staining for Nucleolar Organizer Regions. Trude Schwarzacher and Molecular Cytogenetics Research Group - [Read Silver Staining for Nucleolar Organizer Regions]

Single Colour Fish Analysis Method - http://www.sanger.ac.uk/HGP/methods/cytogenetics/single_colour.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Single colour detection for biotinylated probes. Sanger Institute - [Read Single Colour Fish Analysis Method]

Slide Preparation for Cytogenetics Protocol - http://info.med.yale.edu/genetics/ward/tavi/Cytom-slideprep.html

Category: Cytogenetics Protocols

Protocol for slide preparation for cytogenetics. Includes: Improved cell recovery by microcentrifugation; Slide storage; "Controlled" chromosome spreading; Dropping cells from a height does not improve spreading; GTG-banding. - [Read Slide Preparation for Cytogenetics Protocol]

Slide preparation for FISH and Cytogenetics - http://www.sanger.ac.uk/HGP/methods/cytogenetics/slide_preparation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Slide preparation for FISH and Cytogenetics. - [Read Slide preparation for FISH and Cytogenetics]

Titration of DNaseI for nick translation - http://www.sanger.ac.uk/HGP/methods/cytogenetics/dnase.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Titration of DNaseI for nick translation. Sanger Institute. - [Read Titration of DNaseI for nick translation]

Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes - http://www.sanger.ac.uk/HGP/methods/cytogenetics/two_colour.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes. Sanger Institute - [Read Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes]

Yeast DNA Isolation Protocol for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/yacs.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Yeast DNA Isolation Protocol for FISH. Sanger Institute. - [Read Yeast DNA Isolation Protocol for FISH]