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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

The Use of Mass Spectrometry in Proteomics: Database Searching - http://www.cshprotocols.org/cgi/content/extract/2006/27/pdb.ip5

Category: Bioinformatics

Searches are not constrained for only tryptic peptides, and indexed databases (databases only containing tryptic peptides) are not used. In cases where there are very complex mixtures, such as cell lysates, nonspecific cleavages can occur. Therefore, nontryptic peptides would be missed in the database search. - [Read The Use of Mass Spectrometry in Proteomics: Database Searching]

The Usefulness of Real-time PCR in Immunogenetics - http://www.ashi-hla.org/publicationfiles/ASHI_Quarterly/25_3_2001/The%20Usefullness%20of%20Quantitative.pdf

Category: Real Time PCR: Real Time PCR Information

The Usefulness of Real-time PCR in Immunogenetics. Principles, technique, applications of Real time pcr. Provenzano et al., Scientific 2001 Communications. - [Read The Usefulness of Real-time PCR in Immunogenetics]

The Virtual Mouse Necropsy - http://www.geocities.com/virtualbiology/

Category: Animal Research Techniques

Perform a Mouse Necropsy on the Web. Dr. J.M.Ward - [Read The Virtual Mouse Necropsy]

The Whole-Cell Patch-Clamp Technique â€“ a powerful tool to approach CFTR function - http://pen2.igc.gulbenkian.pt/cftr/vr/d/hug_whole_cell_patch_clamp_tool_cftr_function.pdf

Category: Electrophysiology Ion Channels

The Whole-Cell Patch-Clamp Technique â€“ a powerful tool to approach CFTR function. Martin J. Hug. University of Muenster, Institute of Physiology. Germany - [Read The Whole-Cell Patch-Clamp Technique â€“ a powerful tool to approach CFTR function]

Theory: How to Make Simple Solutions and Dilutions - http://abacus.bates.edu/~ganderso/biology/resources/dilutions.html

Category: Buffers Media, and Solutions

Theory: How to Make Simple Solutions and Dilutions. Information on dilution making. - [Read Theory: How to Make Simple Solutions and Dilutions]

Thioflavine S Amyloid Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/THIOFLAV.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

Protocol for thioflavine amyloid. - [Read Thioflavine S Amyloid Protocol]

Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells - http://www.natureprotocols.com/2006/11/17/thymosin_4_t4induced_outgrowth.php

Category: Primary Cell Isolation and Culture Protocols

Thymosin β4 (Tβ4)-induced outgrowth and differentiation of vasculogenic precursor cells. - [Read Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells]

Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells Protocol - http://www.natureprotocols.com/2006/11/17/thymosin_4_t4induced_outgrowth.php

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol for thymosin β4 (Tβ4)-induced outgrowth and differentiation of vasculogenic precursor cells from adult epicardium. Includes: Preparation of EPDCs; Characterisation of EPDC Phenotypes by Immunofluorescence. - [Read Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells Protocol]

Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4602

Category: Microscopy Protocols: In Vivo Imaging Protocols

Sophisticated fluorescence microscopy methods & equipment, now allow cellular events to be studied at high resolution in living material. The studying of living fly tissues presents unique difficulties in keeping the cells alive, introducing fluorescent probes, & imaging through thick hazy cytoplasm. This protocol outlines the preparation of major tissue types amenable to study by time-lapse cinematography and different methods for keeping them alive. - [Read Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material]

Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4602

Category: Drosophila Protocols

Protocol outlines the preparation of major tissue types amenable to study by time-lapse cinematography and different methods for keeping them alive. - [Read Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material Protocol]

Timeline for Transgenic Mouse Analysis - http://www.med.umich.edu/tamc/tgtime.html

Category: Transgenic Mouse Protocols

Timeline for Transgenic Mouse Analysis and production. University of Michigan Transgenic Animal Model Core. Transgenic Animal Web. - [Read Timeline for Transgenic Mouse Analysis]

Timing of Cycles Protocol - http://homepages.gac.edu/~cellab/chpts/chpt11/ex11-5.html

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Protcol for the timing of cycles. - [Read Timing of Cycles Protocol]

Timing of Cycles Protocol - http://homepages.gac.edu/~cellab/chpts/chpt11/ex11-5.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Protocol for timing of cycles. - [Read Timing of Cycles Protocol]

Tips and hints for the storage of antibodies - http://www.sysy.com/faqs/storage.html

Category: Protein Protocols: Western Blot Protocol: Antibody Storage Conditions

Tips and hints for the storage of antibodie. Storage of lyophilized antibodies, - [Read Tips and hints for the storage of antibodies]

Tips and Hints for the Storage of Antibodies - http://www.sysy.com/faqs/storage.html

Category: Antibody Protocols: Antibody Production Protocols

Tips and hints for the storage of antibodies. Includes: Storage of lyophilized antibodies; Monoclonal antibodies; Rabbit antisera; Polyclonal affinity purified antibodies. - [Read Tips and Hints for the Storage of Antibodies]

Tips for Sample Preparation for MALDI mass spectrometry - http://www.cfgbiotech.com/proteomics/sample_prep.htm

Category: Mass Spectrometry Protocols

Tips for Sample Preparation for MALDI mass spectrometry. Sample quantity, Solvent, Protein/Peptide sample handling. U. Albany. CFG - [Read Tips for Sample Preparation for MALDI mass spectrometry]

Tissue Culture Methods - http://www.research.umbc.edu/~jwolf/method5.htm#tc%20methods

Category: Cell Biology and Cell Culture: Cell Culture Techniques

Tissue Culture Methods. Julie B. Wolf, Univ. Maryland. Protocols for Counting Cells, Preservation and storage of cell lines, maintenance of cell lines, media and growth, and safety guidelines for working with cells. - [Read Tissue Culture Methods]

Tissue sectioning and in situ hybridization as prepared by Simon Dunn PDF - http://oregonstate.edu/~weisv/Protocols/Cellular/Tissue%20sectioning%20and%20in%20situ%20hybridization%20Protocol.pdf

Category: Histology Protocols: Tissue Sample Sectioning

Tissue sectioning and in situ hybridization as prepared by Simon Dunn PDF. Includes Reagents and Solution recipes. Weis Lab, Virginia. - [Read Tissue sectioning and in situ hybridization as prepared by Simon Dunn PDF]

Tissue Stain for Axons Protocol - http://www.nottingham.ac.uk/pathology/protocols/biels.html

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

Protocol for tissue stain for axons and Alzheimer's disease. - [Read Tissue Stain for Axons Protocol]

Tissue Stain for Nissl Substance - http://www.nottingham.ac.uk/pathology/protocols/cfvnissl.html

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protocol for tissue stain for Nissel substance usin cresyl fast violet technique. - [Read Tissue Stain for Nissl Substance]

Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomic - http://www.nature.com/nprot/journal/v1/n4/abs/nprot.2006.273.html

Category: Protein Protocols: Protein Extraction From Tissue

Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomics. Although many methods exist for fractionating proteins, the method described here can capture the majority of subcellular fractions simultaneously at reasonable purity. The scalability of this method makes it amenable to small samples, such as embryonic tissues, in addition to larger tissues. The protocol described is for the general fractionation and extraction of proteins from organs / tissue - [Read Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomic]

Titering & Propagation of Phage - http://www.rpgroup.caltech.edu/~paul/protocols/phage/propagation/

Category: Molecular Biology Protocols: Phage Protocols and Methods

Titering" a phage means measuring the concentration of phage in a given solution. Titering Procedure. Rob Philip's Group. - [Read Titering & Propagation of Phage]

Titering of Bacterial Viruses Protocol - http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Phage/Phage_Titer.htm

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

When an individual bacterial virus grows in a bacterial host suspended in a top agar lawn, its progeny infect and lyse the surrounding host cells. This causes the appearance of a "hole" or plaque in the otherwise homogeneous bacterial lawn. Since each plaque represents a single virus, the number of viruses in the aliquot added to the plate is equal to the number of plaques which appear. - [Read Titering of Bacterial Viruses Protocol]

Titration of DNaseI for nick translation - http://www.sanger.ac.uk/HGP/methods/cytogenetics/dnase.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Titration of DNaseI for nick translation. Sanger Institute. - [Read Titration of DNaseI for nick translation]

Titration of Retrovirus Protocol - http://www.methodbook.net/virus/titratn.html

Category: Viral Protocols

Protocol for the titration of retrovirus. - [Read Titration of Retrovirus Protocol]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.