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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Sorting Aberrant Cells Using Anti-Cytokeratin or DNA Content for Study of Cell Morphology - http://www.bdbiosciences.com/pdfs/whitePapers/23-2786-02.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Demonstrates the use of the FACSort for sorting based on DNA ploidy or the presence of cytokeratin, and the suitability of the sorted cells for morphologic analysis by microscopic examination. - [Read Sorting Aberrant Cells Using Anti-Cytokeratin or DNA Content for Study of Cell Morphology]

Sorting Transfected Cells Based on Gene Expression, Followed by Culture in Vivo - http://www.bdbiosciences.com/pdfs/whitePapers/23-2787-02.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Describes how FACSort can be used to enrich for transfected mouse cells expressing high levels of the human thrombin receptor. The sorted fraction can then be cultured in vivo and reanalyzed 12 days later to show that it remains enriched for thrombin receptor-expressing cells. - [Read Sorting Transfected Cells Based on Gene Expression, Followed by Culture in Vivo]

Southern Blotting Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S5.html

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for southern blotting. - [Read Southern Blotting Protocol]

Southern Blotting: Capillary Transfer of DNA to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4040

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol describes the first stages of Southern blotting: digestion of genomic DNA with one or more restriction enzymes, separation of the resulting fragments by electrophoresis through an agarose gel, and transfer of the denatured fragments to a membrane by downward capillary transfer. - [Read Southern Blotting: Capillary Transfer of DNA to Membranes Protocol]

Southern Blotting: Simultaneous Transfer of DNA from a Single Agarose Gel to Two Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4043

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for southern blotting: simultaneous transfer of DNA from a single agarose gel to two membranes. DNA can be simultaneously transferred from opposite sides of a single agarose gel to two membranes. Bidirectional transfer occurs rapidly at first, but soon slows down as the gel becomes dehydrated. Because the efficiency of transfer is low, the method works best when the target sequences are present in high concentration - [Read Southern Blotting: Simultaneous Transfer of DNA from a Single Agarose Gel to Two Membranes Protocol]

Southern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4044

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for southern hybridization of radiolabeled probes to nucleic acids immobilized on membranes. Protocol describes how to carry out Southern hybridizations at high stringency in phosphate-SDS buffers. Although a wide variety of formats are available, most Southern hybridizations are carried out in heat-sealable bags, roller bottles, or plastic boxes. - [Read Southern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes Protocol]

Southgates Mucicarmine Protocol - http://www.nottingham.ac.uk/pathology/protocols/mucicarm.html

Category: Neuroscience Protocols: Histology Stains Protocols: Mucin Stains Protocols

Protocol for southgates mucicarmine. - [Read Southgates Mucicarmine Protocol]

Southwestern Blot Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S3.html

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for southwestern blot. - [Read Southwestern Blot Protocol]

Soybean Cotyledonary-node Agrobacterium-mediated Transformation System - http://www.biotech.unl.edu/transgenic/protocols.html

Category: Plant Biology Protocols

Protocol includes: Seed Sterilization Step; Seed Germination Step; Agrobacterium Inoculum Preparation; Explant Preparation; Co-cultivation Plates; Shoot Initiation Step; Shoot Elongation Step; Rooting Step. - [Read Soybean Cotyledonary-node Agrobacterium-mediated Transformation System]

Special Stains in Histology - http://medlib.med.utah.edu/WebPath/HISTHTML/STAINS/STAINS.html

Category: Histology Protocols

Special Stains in Histology. Mucin stains, Melanin stains, etc. - [Read Special Stains in Histology]

Spectral Karyotyping Mouse Protocol - http://www.jax.org/staff/mills/protocols/spectral_karyotyping_mouse.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for spectral karyotyping mouse. - [Read Spectral Karyotyping Mouse Protocol]

Spectrophotometric Measurement of Nucleic Acids' Concentration Tool - http://molbiol.ru/eng/scripts/01_03.html

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Spectrophotometric Measurement of Nucleic Acids' Concentration Tool. This bioinformatic program help calculate the concentration of nucleic acids according to optical density (including DNA, RNA, oligonucleotides). Zbio - [Read Spectrophotometric Measurement of Nucleic Acids' Concentration Tool]

Spectrophotometry - http://homepages.gac.edu/~cellab/appds/appd-g.html

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Bradford Protein Assay, Lowry Protein Assay, Biuret Assay. Protein concentration measurement. Dr. Heidcamp, Department of Biology, Gustavus Adolphus College. - [Read Spectrophotometry]

Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells Protocol - http://www.duke.edu/web/ceramide/protocols/0040.html

Category: Lipid Protocols

Protocol for sphingomyelin quantitation post-choline labeling of HL60 cells. - [Read Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells Protocol]

Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Cells - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4596

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol describes a split luciferase complementation assay used to study the interaction of proteins in cells. In the split protein strategy, a single reporter protein/enzyme (firefly luciferase [Fluc]) is cleaved into amino-terminal and carboxy-terminal halves; each half is fused to one of two interacting proteins, X & Y. Physical interactions between the two proteins reconstitute the functional reporter protein, leading to enzymatic activities that can be measured by in vitro or in vivo assay - [Read Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Cells]

Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Living Mice - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4595

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol describes a split luciferase complementation assay that can be used to repetitively and noninvasively study the interaction of proteins in small living animals. After the expression of the appropriate vectors has been checked in cell culture in vivo, studies can be performed either by implanting transiently transfected cells for short-term analysis (maximum of 7 days), or with tumor models grown from tumor cells stably expressing the complete reporter system. - [Read Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Living Mice]

Splitting Human ES cells on MEFs Protocol - http://ink.primate.wisc.edu/~thomson/pdfs/splitting_es.pdf

Category: Stem Cell Protocols: Mouse Embryonic Fibroblasts Protocols

Protocol for splitting human ES cells on MEFs. - [Read Splitting Human ES cells on MEFs Protocol]

Spore Germination Protocol - http://www-rcf.usc.edu/~forsburg/spores.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for spore germination. This procedure is typically used for the isolation and preparation of spores from a diploid strain heterozygous for a marked disruption (e.g., yfg1::his3+) Inoculation of the spore population into minimal medium lacking the nutritional supplement corresponding to the disruption marker (e.g., minimal medium lacking histidine) allows only the disruption spores to germinate. - [Read Spore Germination Protocol]

Sporulation and Dissection of Yeast a/alpha Diploids Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_sporulation.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for sporulation and dissection of yeast a/alpha diploids. - [Read Sporulation and Dissection of Yeast a/alpha Diploids Protocol]

Sporulation and Tetrad Dissection Protocol - http://www.biochem.emory.edu/labs/acorbe2/yeastsporulation.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for sporulation and tetrad dissection. - [Read Sporulation and Tetrad Dissection Protocol]

Sporulation Guide - http://www.genomics.princeton.edu/dunham/sporulationdissection.htm

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocols and Information on sporulation. Includes: SPO++ media; 10X amino acid stock; Sporulation; Dissection. - [Read Sporulation Guide]

Sporulation Protocols - http://www.fhcrc.org/science/labs/gottschling/yeast/spo.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocols for sporulation. Includes yeast vivisection. - [Read Sporulation Protocols]

Spotted Microarray Hybridization and Scan Protocol - http://www.genome.wisc.edu/resources/protocols/spottedarrayhybridization.htm

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol for spotted microarray hybridization and scan. Includes: Setup Hybridization; Wash Slides; Scan Slides. - [Read Spotted Microarray Hybridization and Scan Protocol]

Squash Preparation of Plant Chromosomes Protocol - http://www.le.ac.uk/bl/phh4/prosquash.htm

Category: Plant Biology Protocols: Plant Genetics Protocols

Protocol for the preparation of plant chromosomes using squash. - [Read Squash Preparation of Plant Chromosomes Protocol]

Squash Preparation of Plant Chromosomes Protocol - http://www.le.ac.uk/bl/phh4/prosquash.htm

Category: Cytogenetics Protocols

Protocol for squash preparation of plant chromosomes. - [Read Squash Preparation of Plant Chromosomes Protocol]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.