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Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/telford/cyclinip.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Protocol for immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry. - [Read Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry Protocol]

Immunohistochemistry Milk Method with 0.01% Triton X-100 Protocol - http://www.bcm.edu/rosenlab/protocols/milkmethod.pdf

Category: Immunohistochemistry Protocols

Immunohistochemistry using the “milk method” with 0.01% triton X-100. This method works for the following antibodies: p27, cyclin D1, RbAP46. - [Read Immunohistochemistry Milk Method with 0.01% Triton X-100 Protocol]

Phenotype-Specific Immunodetection of Cyclins using 488/630 nm Dual Laser Flow Cytometry Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/telford/cyclind.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Protocol for phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry. This protocol is for use with the D and E cyclins and employs 488 nm argon laser excitation of propidium iodide and a FITC-conjugated phenotypic label, and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression. - [Read Phenotype-Specific Immunodetection of Cyclins using 488/630 nm Dual Laser Flow Cytometry Protocol]

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Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

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