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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Cell Culture Phototoxicity Test Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/44.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Human A431 cells and mouse 3T3 cells are exposed in culture to UV light both in the presence and absence of test compound. Phototoxicity is expressed as a decrease in cell viability as determined by the MTT assay. - [Read Cell Culture Phototoxicity Test Protocol]

Cell Culture Protocol - http://www.well.ox.ac.uk/flint/CellCulture.htm

Category: Cell Biology and Cell Culture

Cell Culture Protocol. A short protocol. - [Read Cell Culture Protocol]

Cell Culture Protocols. Basic Topics in PDF - http://www.cf.ac.uk/phrmy/PCB/PageCellCultProtocols.html

Category: Cell Biology and Cell Culture

Cell Culture Protocols. Basic Topics in PDF. Gumbleton Lab PCB. - [Read Cell Culture Protocols. Basic Topics in PDF]

Cell Culture Trypsinization Subculturing MolecularStation - http://www.molecularstation.com/cell/cell-culture-protocols/

Category: Cell Biology and Cell Culture

Cell Culture Trypsinization Subculturing MolecularStation. - [Read Cell Culture Trypsinization Subculturing MolecularStation]

Cell Cycle Analysis - Propidium Iodide - http://science.cancerresearchuk.org/sci/facs/facs_major_apps/cell_cycle_analysis/propidium_iodide/?version=1

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Information on how to quantitatively assess DNA content. Includes: Propidium iodide staining protocol and staining of DNA by Mithramicin Protocol - [Read Cell Cycle Analysis - Propidium Iodide]

Cell Cycle Analysis - Propidium Iodide - http://science.cancerresearchuk.org/sci/facs/facs_major_apps/cell_cycle_analysis/propidium_iodide/?version=1

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Information on propidium iodide for cell cycle analysis. - [Read Cell Cycle Analysis - Propidium Iodide]

Cell Cycle Protocol using Flow Cytometry - http://www.people.cornell.edu/pages/jls269/protocols/cellcycleprotocols.html

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Cell Cycle Protocol using Flow Cytometry. Cell cycle Analysis by Flow Cytometry. DNA content Analysis on the FACSAria. Analyzing Cell cycle FCM data. Biomedical Sciences Flow Cytometry Core Laboratory. Dr.Smith Cornell. - [Read Cell Cycle Protocol using Flow Cytometry]

Cell Disruption and Subcellular Fractionation Protocol - http://www.duke.edu/web/ceramide/protocols/0011.html

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols

Protocol for cell disruption and subcellular fractionation. Includes: Nitrogen Bomb, Fractionation and Reagents. - [Read Cell Disruption and Subcellular Fractionation Protocol]

Cell Fixation Using Methanol - http://www.lamondlab.com/pdf/CellFixMEOH.pdf

Category: Immunology: Immunocytochemistry Protocols

Methanol Fixation for Immunofluorescence. Lamond Lab, Univ. Dundee - [Read Cell Fixation Using Methanol]

Cell Fractionation of a Mixed Population of Cells - http://www.axis-shield.com/densityhome/optiprep/C14.pdf

Category: Cell Biology and Cell Culture

Cell fractionation techniques are presented for the design of gradient systems for separating one or more cell types from lavages of body cavities or from mechanically or enzymically-dissociated tissues. Includes: Preparation of cell suspension for gradient loading; Fractionation by buoyant density; Fractionation on the basis of cell size. - [Read Cell Fractionation of a Mixed Population of Cells]

Cell Fractionation protocol Usingt Low Speed Separaton of Cells - http://homepages.gac.edu/~cellab/chpts/chpt3/ex3-4.html

Category: Cell Biology and Cell Culture

Cell fractionation protocol using low speed seperation. - [Read Cell Fractionation protocol Usingt Low Speed Separaton of Cells]

Cell Freezing using Liquid Nitrogen N2 - http://www.liv.ac.uk/~giles/methods/cell_freezing.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing gealthy cells (> 95% viable) the protocol obtains a culture 80-90% viable 24 hours after thawing and growing on test vial from step 9. Antisense Research Group - [Read Cell Freezing using Liquid Nitrogen N2]

Cell Immortalization - http://www.atcc.org/common/products/CellImmortOverview.cfm

Category: Cell Immortalization Protocols

Information why cell immortalization is important. Also the available tools to be able to perform cell immortalization. - [Read Cell Immortalization]

Cell Lysate Extracts - http://www.upstate.com/misc/protocol_detail.q.prot.e.CellLysate_Prep.a.name.e.Cell_Lysate_Extracts-General_Protocols

Category: Protein Protocols: Protein Extraction From Cells

Cell Lysate Extracts. Great protocols for cell lysis preparation from a variety of cell types. There are numerous methods of cell stimulation and lysis. For a given protein, Upstateâ€™s Laboratories determine the specific treatment upon initial testing of its products. It is important to select the correct cell line, stimulation procedure (if any), and lysis protocol. Upstate. - [Read Cell Lysate Extracts]

Cell Lysates For Western Blotting - http://www.liv.ac.uk/~giles/methods/western_lysate.htm

Category: Protein Protocols: Western Blot Protocol

How to prepare cell lysates for western blotting. - [Read Cell Lysates For Western Blotting]

Cell Mass by Measurement of Turbidity - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-5.html

Category: Cell Biology and Cell Culture

Cell Mass by Measurement of Turbidity. Cell Biology Laboratory Manual. Heidcamp - [Read Cell Mass by Measurement of Turbidity]

Cell Mass by Measurement of Turbidity Protocol - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-5.html

Category: Bacterial Cell Culture Protocols

Protocol for measurement of cell mass by turbidity. - [Read Cell Mass by Measurement of Turbidity Protocol]

Cell Staining for Immunofluorescence Microscopy - http://www.bdbiosciences.com/pharmingen/protocols/Immunofluorescence_Microscopy.shtml

Category: Molecular Imaging: Immunofluorescence Microscopy

Cell Staining for Immunofluorescence Microscopy. Includes protocols for fixing the cells, Coverslip Preparation for Adherent Cells, Coverslip Preparation for Non-Adherent Cells, Paraformaldehyde Fixation, and Methanol/Acetone Fixation. Blocking protocols include blocking with primary antibody, and incubation with secondary antibody. - [Read Cell Staining for Immunofluorescence Microscopy]

Cell Staining for Sorting of Hematopoietic Stem Cells (HSC) and Myeloid Progenitors - http://www.natureprotocols.com/2006/06/29/cell_staining_for_sorting_of_h.php

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

The combination of prospective identification/isolation of bone marrow progenitors and quantitative RT-PCR is a powerful tool to understand the molecular mechanism underlying hematopoiesis. Describes the standard procedures of the murine myeloid progenitor staining for fluorescence activated cells sorting (FACS) and RNA purification methods. - [Read Cell Staining for Sorting of Hematopoietic Stem Cells (HSC) and Myeloid Progenitors]

Cell Staining for Sorting of Hematopoietic Stem Cells and Myeloid Progenitors and Isolating RNA - http://www.natureprotocols.com/2006/06/29/cell_staining_for_sorting_of_h.php

Category: Stem Cell Protocols

The combination of prospective identification/isolation of bone marrow progenitors and quantitative RT-PCR is a powerful tool to understand the molecular mechanism underlying hematopoiesis. Here, we described our standard procedures of the murine myeloid progenitor staining for fluorescence activated cells sorting (FACS) and RNA purification methods. - [Read Cell Staining for Sorting of Hematopoietic Stem Cells and Myeloid Progenitors and Isolating RNA]

Cell Surface Labeling Protocol - http://sciencepark.mdanderson.org/fcores/flow/files/Cell%20Surface%20Labeling.pdf

Category: Fluorescent Dyes Protocols

Protocol for cell surface labeling. - [Read Cell Surface Labeling Protocol]

Cell Synchronization Using Centrifugal Elutriation Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4490

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

protocol describes a method for the synchronization of cell populations using centrifugal elutriation. The method relies on the fact that cell size increases linearly as cells proceed through the cell cycle. Cells of similar size (and cell cycle phase) are eluted stepwise from the cell chamber, with the smallest size (those in early G1) being eluted first. Using this procedure, it is possible to obtain relatively pure populations of cells at various points in G1, S, and G2/M. - [Read Cell Synchronization Using Centrifugal Elutriation Protocol]

Cell Synchronization Using Centrifugal Elutriation Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4490

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

This protocol describes a method for the synchronization of cell populations using centrifugal elutriation. The method relies on the fact that cell size increases linearly as cells proceed through the cell cycle. Cells of similar size (and cell cycle phase) are eluted stepwise from the cell chamber, with the smallest size (those in early G1) being eluted first. Using this procedure, it is possible to obtain relatively pure populations of cells at various points in G1, S, and G2/M. - [Read Cell Synchronization Using Centrifugal Elutriation Protocol]

Cell Thawing and cell line Freezing Protocol - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/FreezeThawProtocol.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Method for freezing and thawing cells. Immunology Applications Core Facility. U. Chicago - [Read Cell Thawing and cell line Freezing Protocol]

Cell Viability Assays that Measure ATP Protocol - http://www.promega.com/paguide/chap4.htm#title2

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method to determine the number of viable cells in culture. Detection is based on using the luciferase reaction to measure the amount of ATP from viable cells. The amount of ATP in cells correlates with cell viability. - [Read Cell Viability Assays that Measure ATP Protocol]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.