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Cutting Bands and Reamplification for Sequencing Protocol - http://genome-lab.ucdavis.edu/Protocols/Cutting%20Bands%20and%20Reamplification%20for%20Sequencing.htm

Category: Genetics and Genomics: Cancer Genetics Protocols

Protocol for cutting bands and reamplification for sequencing. - [Read Cutting Bands and Reamplification for Sequencing Protocol]

DNA Extraction from Agarose Gels Protocol - http://www.methodbook.net/dna/gelextrc.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

DNA Extraction from Agarose Gels Protocol. The page includes cutting out the DNA band from the gel, and describes three methods including 1) Spin-columns (Nucleic acid purification columns), 2) using Dialysis tubing (semi-permeable membrane, Visking tubing), and the 3) Paper strip method.Matt Lewis, Department of Pathology, University of Liverpool. - [Read DNA Extraction from Agarose Gels Protocol]

High Molecular Weight Yeast Liquid DNA Preparation Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast4.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol that isolates intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. Using this protocol one can expect a yield of 100-200 µg of DNA per prep. - [Read High Molecular Weight Yeast Liquid DNA Preparation Protocol]

Laser-capture Microdissection Protocol - http://www.nature.com/nprot/journal/v1/n2/full/nprot.2006.85.html#a1

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity (LOH) analysis, etc. Protocol provides a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1–1.5 h. - [Read Laser-capture Microdissection Protocol]

Low-melt Polyester Embedding Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/LowMeltPolyester.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols: Tissue Preparation for LCM Protocols

Protocol for low-melt polyester embedding. Includes: Large Tissue Specimens; Small Tissue Specimens and Biopsies; Histology Slide Cutting Procedure. - [Read Low-melt Polyester Embedding Protocol]

Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections Protocol - http://dir.nichd.nih.gov/lcm/LCMTAP.htm#I.%20Preparation,%20LCM%20and%20RNA/DNA%20extraction%20of%20Frozen%20Tissue%20Sections

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Protocol for the preparation, LCM and RNA/DNA extraction of frozen tissue sections. Includes: Embedding, Cutting and Staining. - [Read Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections Protocol]

Restriction Landmark Genomic Scanning Protocol - http://www.natureprotocols.com/2007/01/25/restriction_landmark_genomic_s.php

Category: Nucleic Acid Detection Protocols

Restriction landmark genomic scanning (RLGS) is a method to detect large numbers of restriction landmarks in a single experiment. It is based on the concept that restriction enzyme sites can serve as landmarks throughout a genome. RLGS uses direct end-labeling of the genomic DNA digested with a rare-cutting restriction enzyme and high-resolution two-dimensional electrophoresis. - [Read Restriction Landmark Genomic Scanning Protocol]