culturing Protocols

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Analysis of Cellular DNA Content by Flow Cytometry Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C8A9C23374B5FAB0610666CCDFB&objectid=6674F58BDEF6DCA5276258FEA4CD534D

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

Simple and universally applicable methods for staining fixed cells are presented, as are methods that utilize detergents and proteolytic treatment to permeabilize cells. Additionally, supravital cell staining with Hoechst 33342, which is primarily used for sorting live cells for subsequent culturing based on DNA-content differences, is also described. Also presented are methods for staining of cell nuclei isolated from paraffin-embedded tissues, and deconvolution of DNA-content-frequency... - [Read Analysis of Cellular DNA Content by Flow Cytometry Protocol]

Basic Methods of Culturing Drosophila Protocol - http://fly.bio.indiana.edu/Fly_Work/culturing.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for the basic methods of culturing Drosophila. Includes: Stockkeeping; Culture contaminants; Experimental populations. - [Read Basic Methods of Culturing Drosophila Protocol]

Culturing Marine Euplotids Using Dunaliella as a Food Source Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4467

Category: Laboratory Model Organisms Protocols

Protocol describes the culture of marine euplotids using Dunaliella salina or D. tetiolecta as a food organism. Dunaliella tolerate a wide range of salinity, thus they are fairly easy to grow in the lab using artificial sea salts. - [Read Culturing Marine Euplotids Using Dunaliella as a Food Source Protocol]

Culturing of Insect Cells and Production of Baculovirus Expressed Recombinant Proteins in 2.8 Liter - http://www.hyclone.com/pdf/procedure_insect_fernbach.pdf

Category: Cell Culture Protocols: Insect Cell Culture Protocols

The use of Ferbach flasks is an ideal method to amplify the cells and virus inoculum for the bioreactor. The method is also very practical for producing sufficient amounts of various proteins for feasibility studies using HyQ© SFX-Insect™ medium. The large surface area in this vessel enables sufficient aeration and, therefore, the cells, when they reach high density, are not oxygen depleted. - [Read Culturing of Insect Cells and Production of Baculovirus Expressed Recombinant Proteins in 2.8 Liter]

Culturing Schwann Cells from Adult Peripheral Nerve Protocol - http://www.physiol.usyd.edu.au/daved/papers/1998/sc_culture/

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: Rat Neuronal Cell Culture Protocols

Protocol for culturing schwann cells from adult peripheral nerve. - [Read Culturing Schwann Cells from Adult Peripheral Nerve Protocol]

Culturing Trophoblast Stem (TS) Cell Lines Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4406

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Culture conditions have been established for a second blastocyst-derived cell line, trophoblast stem (TS) cells, in addition to embryonic stem (ES) cells. This protocol describes a method for culturing TS cell lines. These cells can then be used to study trophoblast differentiation and placental function. - [Read Culturing Trophoblast Stem (TS) Cell Lines Protocol]

Germ Layer Explant Recombination Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4369

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This protocol describes a method for recombining and culturing germ layer fragments. It is useful for testing the inductive properties of fragments from wild-type and mutant mouse embryos. - [Read Germ Layer Explant Recombination Culture Protocol]

Germ Layer Explant Recombination Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4369

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol describes a method for recombining and culturing germ layer fragments. It is useful for testing the inductive properties of fragments from wild-type and mutant mouse embryos. - [Read Germ Layer Explant Recombination Culture Protocol]

Growth and Concentration of Chlorogonium for Culturing Freshwater Hypotrichs Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4464

Category: Laboratory Model Organisms Protocols

Protocol describes the growth and concentration of the alga Chlorogonium elongatum as a food source for culturing freshwater hypotrichs. Most freshwater hypotrichs (including Oxytricha nova, O. fallax, and O. trifallax; Euplotes aediculatus and E. eurystomous; and Stylonychia lemnae) can be grown to high density with Chlorogonium as the food organism. A similar regimen can be used to prepare other food sources such as Tetrahymena or bacteria (e.g., Aerobacter aerogenes). - [Read Growth and Concentration of Chlorogonium for Culturing Freshwater Hypotrichs Protocol]

Human Thyroid Culture Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/29.htm#TOP

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This method enables the culturing of thyroid cells without loss of differentiation and medium change. It is potentially useful for the long-term study of drug effects on the thyroid gland. - [Read Human Thyroid Culture Protocol]

IMEC Maintenance - http://www.flemingtonlab.com/Protocols/IMECMaintCulturingMisc.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Information on Immortalized Mammary Epithelial Cells (IMEC). Includes: Background Information on IMEC; Culturing; Trypsinization. - [Read IMEC Maintenance]

Large List of ES Cell Culturing - http://web.archive.org/web/20030201220413/grimwade.biochem.unimelb.edu.au/bowtell/cellbiol/cellbio.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Large List of ES Cell Culturing. Bowtell Lab. - [Read Large List of ES Cell Culturing]

Neuron Culture Solutions - http://www.ehlerslab.org/protocols.html

Category: Neuroscience: Neuron Cell Culture Protocols

Neuron Cell Culture Solutions, Neuron Culturing, Preparation of Brain Membrane Fractions, Biotinylation Assay of Receptor Endocytosis, PSD Prep. Ehlers lab Duke Univ. - [Read Neuron Culture Solutions]

Preparation of Seeded NGM Plates For Worm Food Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1558

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol describes how to seed plates with E. coli OP50 for C. elegans cultures. Includes: Bacterial Stock Plate Preparation; Bacterial Broth Preparation; Seeding Bacteria Plates for Culturing Worms. - [Read Preparation of Seeded NGM Plates For Worm Food Protocol]

Protocols for Insect Cell Culture - http://www.hyclone.com/library/insectprotocols.htm

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Includes protocols: Direct Adaptation to HyQ© SFX-Insect™ Medium; Sequential Adaptation to HyQ© SFX-Insect™ Medium; Adaptation of High Five™ Cells to HyQ© SFX-Insect™; Production of Recombinant Protein Using the Baculovirus Expression Vector System; Culturing Insect Cells and Production of Baculovirus Expressed Proteins in 2.8 L Fernbach Systems. Production of Autographa californica Multiple Capsid Nuclear Polyhedrosis Virus (rAcMNPV) Stock - [Read Protocols for Insect Cell Culture]

Rat Chromaffin Cells Primary Cultures Protocol - http://www.natureprotocols.com/2006/09/29/rat_chromaffin_cells_primary_c.php

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol for rat Chromaffin cells primary cultures: Standardization and quality assessment for single-cell assays. Includes: Preliminars; Isolation of the rat adrenal medulla; Enzymatic digestion of medullary tissue; Collecting and culturing the cells. - [Read Rat Chromaffin Cells Primary Cultures Protocol]

Successfully culturing Schwann cells from adult peripheral nerve - http://www.physiol.usyd.edu.au/daved/papers/1998/sc_culture/

Category: Neuroscience: Neuron Cell Culture Protocols

Preparation of cultures, Ansselin et al., 1998. Acta Chururgica Austriaca 30. - [Read Successfully culturing Schwann cells from adult peripheral nerve]