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Mouse ES Cell Culture Techniques - http://imgen.bcm.tmc.edu/molgen/labs/bradley/protocol.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Mouse ES Cell Culture Techniques. List of protocols. The Bradley Lab. - [Read Mouse ES Cell Culture Techniques]

MS Plant Tissue Culture Medium Protocol - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Materials/MSmedium.html

Category: Plant Biology Protocols: Plant Tissue Culture

A recipe for MS plant medium. Details. Details of this protocol, MS Plant Tissue Culture Medium MS Plant Tissue Culture Medium Protocol - [Read MS Plant Tissue Culture Medium Protocol]

Mycoplasma Detection Using DNA Staining Protocol - http://www.corning.com/Lifesciences/technical_information/techDocs/Mycoplasma_testing_protocol.pdf

Category: Cell Culture Protocols: Cell Culture Contamination Prevention and Removal Protocols

One of the most important, but frequently overlooked, cell culture procedures is testing cultures for microbial contamination, especially mycoplasma. It is critical for every cell culture laboratory to only use cell lines that have been carefully screened for mycoplasma. Fortunately, there is a simple fluorochrome DNA staining test that can detect both mycoplasma and virtually any other prokaryote contaminants. - [Read Mycoplasma Detection Using DNA Staining Protocol]

National Centre for Biotechnology Education | Protocols | Plant tissue culture - http://www.ncbe.reading.ac.uk/NCBE/PROTOCOLS/planttissue.html

Category: Plant Biology Protocols: Plant Tissue Culture

Plant tissue culture. *. Unpublished protocols ... ;Plant tissue culture was highly influential in the UK during the 1980s and early... National Centre for Biotechnology Education | Protocols | Plant tissue culture. - [Read National Centre for Biotechnology Education | Protocols | Plant tissue culture]

Neural Stem Cell Culture: Neurosphere Generation, Microscopical Analysis and Cryopreservation - http://www.natureprotocols.com/2006/08/25/neural_stem_cell_culture_neuro.php

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Describes the steps in detail to isolate and expand neural stem cells in the form of neurospheres from tissue dissections of the post-natal mouse brain. Procedures for the long term passage of neurospheres and the cryopreservation of neurospheres are also provided. In addition to the guidelines and tips for generating neurosphere cultures, we describe the method to prepare neurospheres for analysis by light microscopy. - [Read Neural Stem Cell Culture: Neurosphere Generation, Microscopical Analysis and Cryopreservation]

Neuron Culture Solutions - http://www.ehlerslab.org/protocols.html

Category: Neuroscience: Neuron Cell Culture Protocols

Neuron Cell Culture Solutions, Neuron Culturing, Preparation of Brain Membrane Fractions, Biotinylation Assay of Receptor Endocytosis, PSD Prep. Ehlers lab Duke Univ. - [Read Neuron Culture Solutions]

Neutral Red Cytotoxicity Assay Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/64.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

The cytotoxic effect of chemicals upon mammalian cells, such as BALB/c 3T3 and HepG2, in culture is measured by highest tolerated dose (HTD), cell viability (Neutral Red) and total cell protein (coomassie blue). - [Read Neutral Red Cytotoxicity Assay Protocol]

Open Directory - Science: Biology: Botany: Tissue Culture - http://dmoz.org/Science/Biology/Botany/Tissue_Culture/

Category: Plant Biology Protocols: Plant Tissue Culture

Plant Tissue Culture - Laboratory protocol for micropropagation from stem tip, node, meristem, embryo or seed. Includes forum maintained by the National ... - [Read Open Directory - Science: Biology: Botany: Tissue Culture]

Orchid Plant Culture Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Orchid_Culture_Media.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for orchid plant culture media. - [Read Orchid Plant Culture Media Protocol]

Passage of Adherent Cell Lines (subculture). - http://www.hyclone.com/pdf/procedure_passage.pdf

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Remove medium from culture container, rinse with PBS, expose cells to trypsin, incubate, stop trypsin activity by adding serum containing medium, dissociate cells, dilute and return to incubator. - [Read Passage of Adherent Cell Lines (subculture).]

Passage Procedure for RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000159.pdf?pid=PP00000159

Category: Cell Culture Protocols: Cell Lines Protocols

RAW 264.7 cells are a macrophage-like, Abelson leukemia virus transformed cell line derived from BALB/c mice. For routine maintenance in culture (passage), cells are seeded at a confluence of approximately 10% (1 x 106 and 3 x 106 cells in 100-mm and 150-mm plates, respectively) and grown to a confluence of approximately 80%. This procedure requires the cells to be split every two days. - [Read Passage Procedure for RAW 264.7 Cells]

PC12 Cell Culture and Fusion - http://ylwang.umassmed.edu/protocol/cc/pc12.htm

Category: Cell Biology and Cell Culture: Cell Line Culture Conditions

PC12 Cell Culture and Fusion Methods. Yu-li Wang - [Read PC12 Cell Culture and Fusion]

PCR Analysis of Agrobacterium Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4667

Category: Bacteria Genetics Protocols

Protocol describes a method to determine the presence of plasmid DNA in an Agrobacterium culture. Compared to selection of transformed Agrobacterium, which can be ambiguous and normally takes several days for resistant colonies to appear, the approach described here is both rapid and accurate. - [Read PCR Analysis of Agrobacterium Protocol]

PERSPECTIVES ON BACULOVIRUS EXPRESSION SYSTEMS - http://omrf.ouhsc.edu/~frank/baculsum.html

Category: Recombinant Protein Protocols: Baculovirus Recombinant Protein

PERSPECTIVES ON BACULOVIRUS EXPRESSION SYSTEMS. Advantages and disadvantages of protein expression in baculovirus . Insect Cell Culture. Frank Lab. - [Read PERSPECTIVES ON BACULOVIRUS EXPRESSION SYSTEMS]

PLANT CELL, TISSUE AND ORGAN CULTURE - 1998 - http://www.hort.purdue.edu/hort/courses/HORT515/Callus-Ref.html

Category: Plant Biology Protocols: Plant Tissue Culture

Dodds, JH and LW Roberts 1995 Experiments in Plant Tissue Culture. ... 19-60 In Plant Cell Culture Protocols, Humana Press, Totowa ... - [Read PLANT CELL, TISSUE AND ORGAN CULTURE - 1998]

Plant tissue culture - http://www.oup.com/uk/orc/bin/9780199254682/ch02.pdf

Category: Plant Biology Protocols: Plant Tissue Culture

Integration of plant tissue culture into plant. transformation protocols. Various methods of plant regeneration are available to the plant biotechnol- ... - [Read Plant tissue culture]

Plant Tissue Culture Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Media_Preparation.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for plant tissue culture media. Includes: Preparation from Packaged Powder; Preparation from Basal Salt Solutions. - [Read Plant Tissue Culture Media Protocol]

Plant Tissue Culture Protocols. Sigma - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols.html

Category: Cell Biology and Cell Culture

Plant Tissue Culture Protocols. Sigma - [Read Plant Tissue Culture Protocols. Sigma]

Plant Tissue Culture Techniques - http://www.zoo.utoronto.ca/able/volumes/vol-11/9-mineo.pdf

Category: Plant Biology Protocols: Plant Tissue Culture

Plant tissue culture techniques. Pages 151-174, in Tested studies for laboratory ... engineering protocols employ callus initiation procedures after DNA has ... Mineo Zoology Univ. Toronto - [Read Plant Tissue Culture Techniques]

Pouring Plates - http://rothlab.ucdavis.edu/protocols/M10.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

Pouring Plates. Bacterial culture LB. John Roth, U.C Davis. - [Read Pouring Plates]

Preparation and Culture of Cytotrophoblasts Protocol - http://www.natureprotocols.com/2006/08/07/preparation_and_culture_of_cyt.php

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Protocol describes trophoblast cell preparation. - [Read Preparation and Culture of Cytotrophoblasts Protocol]

Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3944

Category: Bacteria Transformation Protocols

Protocol reproducibly generates competent cultures of E. coli that yield 1 x 108 to 3 x 108 transformed colonies/µg of plasmid DNA. The protocol works optimally when the bacterial culture is grown at 18°C. If a suitable incubator is not available, a standard bacterial shaker can be set up in a 4°C cold room and regulated to 18°C. - [Read Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells Protocol]

Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3944

Category: E Coli Protocols: E coli Transformation Protocols

Preparation of DNA from Cells in 24-Well Tissue Culture Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4414

Category: Stem Cell Protocols

This protocol describes a method of DNA preparation from ES cells in 24-well plates. - [Read Preparation of DNA from Cells in 24-Well Tissue Culture Plates Protocol]

Preparation of Double-stranded (Replicative Form) Bacteriophage M13 DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3281

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

The double-stranded replicative form (RF) of bacteriophage M13 is isolated from infected cells using methods similar to those used to purify plasmid DNA. Several micrograms of RF DNA can be isolated from a 1-2-ml culture of infected cells. - [Read Preparation of Double-stranded (Replicative Form) Bacteriophage M13 DNA Protocol]

Articles (1-7 of 7)

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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