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Kitchen Culture Kits - Plant Tissue Culture Resources - http://www.home.turbonet.com/kitchenculture/tcinfo.htm

Category: Plant Biology Protocols: Plant Tissue Culture

Cryopreservation protocols for different kinds of tissue of banana ... Teaching Plant Tissue Culture: University of Liverpool ... - [Read Kitchen Culture Kits - Plant Tissue Culture Resources]

Large-Scale Cell Culture Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662CE6FB2B0DE5496C69D4C4464BA6&objectid=6675A1E8D973D4531201FDB777AF6A84

Category: Cell Culture Protocols

Information on the methods used to cultivate large amounts of cells for the purpose of obtaining an endogenous or recombinant product. - [Read Large-Scale Cell Culture Protocol]

Liquid Culture of Worms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=22

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol for liquid culture of worms. Includes: superbroth; S- basal; worm plates; GROWING THE BACTERIA (WORM FOOD); GROWING THE WORMS; AFTER THE CULTURE HAS GROWN; PREPARING EGGS TO START SYNCHRONIZED LIQUID CULTURES. - [Read Liquid Culture of Worms Protocol]

Liquid Culture of Worms with Fermentor Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=21

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol for liquid culture of worms with fermentor. - [Read Liquid Culture of Worms with Fermentor Protocol]

Live/Dead Assay for Cell Viability Protoco - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000023.pdf?pid=PP00000023

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Assay measures cell viability. It is a two-color fluorescence assay that simultaneously determines Live cell number and Dead cell number. This protocol is designed for use with the GEMINI XS Microplate Spectrofluorometer, a multi-well plate scanner with dual excitation/emission capabilities, but the assay is also adaptable for flow cytometry and fluorescence microscopy. Includes: Cell Culture; Preparation for the Assay; Live/Dead Assay; Reading the Plate; Data Analysis; Alternative protocol. - [Read Live/Dead Assay for Cell Viability Protoco]

Long Term Storage of Yeast Stocks Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast2.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast strains may be stored indefinitely at low temperatures (-80 degrees C). Two archiving methods are presented below. In Method A , the cells are grown on a plate, while in Method B the cells are grown in liquid culture. - [Read Long Term Storage of Yeast Stocks Protocol]

Long-Term Culture of Mouse Cortical Neurons as a Model for Neuronal Development, Aging, and Death - http://neuroscience.jhu.edu/martin9-23.pdf

Category: Neuroscience: Neuron Cell Culture Protocols

Neuronal Cell Culture PDF. Lesuisse and Martin. Wiley Periodicals 2002. - [Read Long-Term Culture of Mouse Cortical Neurons as a Model for Neuronal Development, Aging, and Death]

Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4472

Category: Tetrahymena Protozoa Protocols

Long-term storage of unfrozen Tetrahymena is convenient when a laboratory is not using the cells frequently; however, cells maintained under these conditions often take a couple of days of incubation in culture medium to recover. - [Read Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium Protocol]

Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4471

Category: Tetrahymena Protozoa Protocols

Protocol provides a method for long-term storage of Tetrahymena cultures using maintenance culture medium. - [Read Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium Protocol]

LS-L929 Cytotoxcitiy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/38.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

This simple cell culture-based cytotoxicity test (in which cell viability is determined by uptake of the dyes ethidium bromide and fluorescein acetate) has been developed as a general test for acute toxicity. - [Read LS-L929 Cytotoxcitiy Test]

Lymphocyte Isolation and Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4480

Category: Immunology: Mononuclear Cell Isolation Protocols: B Lymphocyte Isolation Protocols

Protocol describes a method for isolation and stimulation of lymphocytes. Leukocyte-rich plasma is collected from whole blood and then centrifuged through Ficoll-Hypaque. The collected cells are resuspended in growth medium containing various mitogens to stimulate growth. - [Read Lymphocyte Isolation and Culture Protocol]

Lysing Tissue-Culture Cells for Immunoprecipitation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4531

Category: Cell Culture Protocols: Cell Lysis Protocols

For cells grown in tissue culture, the most useful method of lysis is treating with detergents, as described in this protocol. Non-ionic detergents, such as NP-40, solubilize the plasma and intracellular membranes, break many weak intermolecular bonds, and solubilize most of the commonly studied protein antigens. RIPA lysis buffer may be used as a more rigorous extraction buffer to release all but the insoluble proteins of the cell and to break most weak noncovalent interactions. - [Read Lysing Tissue-Culture Cells for Immunoprecipitation Protocol]

Mammalian Cell Culture and Preparation of Cells for RNAi in 24-Well Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4341

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This protocol introduces a method for maintaining mammalian cell culture suitable for treatment with siRNA. Transfection of cultured cells with siRNAs (see Transfection of siRNA Duplexes into Mammalian Cells) and downstream analysis of the knockdown cells. - [Read Mammalian Cell Culture and Preparation of Cells for RNAi in 24-Well Plates Protocol]

Mammalian Cell Culture and Preparation of Cells for RNAi in 24-Well Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4341

Category: RNAi Technology Protocols: RNAi Mammalian Cells Protocols

Protocol introduces a method for maintaining mammalian cell culture suitable for treatment with siRNA. Transfection of cultured cells with siRNAs and downstream analysis of the knockdown cells. - [Read Mammalian Cell Culture and Preparation of Cells for RNAi in 24-Well Plates Protocol]

Mass Culture of Freshwater Hypotrichs Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4466

Category: Laboratory Model Organisms Protocols

Protocol describes the culture of freshwater hypotrichs using the alga Chlorogonium elongatum as a food source. - [Read Mass Culture of Freshwater Hypotrichs Protocol]

Media and Culture of Yeast Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=E5192D47CE9B0776DB7D136C34ED148A&objectid=6673ABA6C2DB2E4E7564EDFA26589256

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

The yeast, Saccharyomyces cerevisiae, has become an important organism in molecular, biochemical, and genetic analysis. The organism has specific requirements for growth under a variety of conditions. The media, both liquid and solid, simple, define, and complex are describe in this unit. Also included are methods for handling, storing, and shipping stock of yeast. - [Read Media and Culture of Yeast Protocol]

Media and Solutions Required for Routine ES Cell Culture Protocol - http://cmbi.bjmu.edu.cn/cmbidata/stem/culture/culture/grimwade.biochem.unimelb.edu.au/bowtell/cellbiol/sect50.htm

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

Protocol for media and solutions required for routine ES cell culture protocol. - [Read Media and Solutions Required for Routine ES Cell Culture Protocol]

Media for Culture of Aspergillus nidulans Protocol - http://www.fgsc.net/methods/anidmed.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for media for culture of Aspergillus nidulans. Includes minimal and complete media. - [Read Media for Culture of Aspergillus nidulans Protocol]

Media for Culture of Aspergillus nidulans Protocol - http://www.fgsc.net/methods/anidmed.html

Category: Fungi Protocols: Aspergillus Protocols

Protocol for media for culture of Aspergillus nidulans. Includes: Minimal medium; Complete media. - [Read Media for Culture of Aspergillus nidulans Protocol]

Media for Culture of Mammalian Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B6F09C1BB8FBC47FC2A7E61A8A3&objectid=66739B890A55AC13354418E67F28E693

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

The culture medium is an essential component of the in vitro environment and must be selected or designed with care. This protocol provides guidelines for design of serum-containing and serum-free media, selective and specialty media, and media for growth under special conditions such as soft-agar growth. - [Read Media for Culture of Mammalian Cells Protocol]

Methods for Synchronizing Cells at Specific Stages of the Cell Cycle - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662F47F15A7860600C31BDC6CCF455&objectid=6673FC95E03763BC6E766F163D5338A0

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Exponentially growing cells are asynchronous with respect to the cell cycle stage. Detection of cell cycle-related events is improved by enriching the culture for cells at the stage during which the particular event occurs. Methods for synchronizing cells are provided here, including those based on morphological features of the cell. - [Read Methods for Synchronizing Cells at Specific Stages of the Cell Cycle]

Mini-Chamber for Regulating Gaseous Environment During Culture - http://www.animal.ufl.edu/hansen/protocols/minigaschamber.htm

Category: Developmental Biology: Embryology Protocols

A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a device. - [Read Mini-Chamber for Regulating Gaseous Environment During Culture]

Mouse Embryonic Fibroblast Culture and Propagation Part II PDF - http://www.wicell.org/uploads/media/Protocols_Part_2_05.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: MEF Protocols Mouse Embryonic Fibroblasts

WiCell Reserarch Institute. Count MEF Cells, Trypsinize, Split, Freeze, Thaw, Irradiate, Reconstitute, Plate, Feeder. - [Read Mouse Embryonic Fibroblast Culture and Propagation Part II PDF]

Mouse embryonic stem (ES) cell culture - basic procedures - http://www.molgen.mpg.de/~rodent/ES_protocol.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Boris Greber. Important rules for ES cell culture, Signs of differentiation, General tips, ES medium, Thawing ES cells, Freezing, Passaging. - [Read Mouse embryonic stem (ES) cell culture - basic procedures]

Mouse embryonic stem (ES) cell culture - basic procedures PDF - http://www.molgen.mpg.de/~rodent/ES_protocol.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Great Procedures. rules for ES cell culture, differentiation of ES cells, ES medium, Thawing, Passaging, Freezing. Mouse embryonic stem (ES) cell culture - basic procedures. Max Planck Institute for Molecular Genetics. Boris Greber. - [Read Mouse embryonic stem (ES) cell culture - basic procedures PDF]

Articles (1-7 of 7)

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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