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Cell Culture Protocol - http://www.well.ox.ac.uk/flint/CellCulture.htm

Category: Cell Biology and Cell Culture

Cell Culture Protocol. A short protocol. - [Read Cell Culture Protocol]

Cell Culture Protocols. Basic Topics in PDF - http://www.cf.ac.uk/phrmy/PCB/PageCellCultProtocols.html

Category: Cell Biology and Cell Culture

Cell Culture Protocols. Basic Topics in PDF. Gumbleton Lab PCB. - [Read Cell Culture Protocols. Basic Topics in PDF]

Cell Culture Trypsinization Subculturing MolecularStation - http://www.molecularstation.com/cell/cell-culture-protocols/

Category: Cell Biology and Cell Culture

Cell Culture Trypsinization Subculturing MolecularStation. - [Read Cell Culture Trypsinization Subculturing MolecularStation]

Cell Freezing using Liquid Nitrogen N2 - http://www.liv.ac.uk/~giles/methods/cell_freezing.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing gealthy cells (> 95% viable) the protocol obtains a culture 80-90% viable 24 hours after thawing and growing on test vial from step 9. Antisense Research Group - [Read Cell Freezing using Liquid Nitrogen N2]

Cell Viability Assays that Measure ATP Protocol - http://www.promega.com/paguide/chap4.htm#title2

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method to determine the number of viable cells in culture. Detection is based on using the luciferase reaction to measure the amount of ATP from viable cells. The amount of ATP in cells correlates with cell viability. - [Read Cell Viability Assays that Measure ATP Protocol]

Cell Viability Assays that Measure Metabolic Capacity Protocol - http://www.promega.com/paguide/chap4.htm#title3

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The CellTiter-Blue® Cell Viability Assay uses an optimized reagent containing resazurin. The homogeneous procedure involves adding the reagent directly to cells in culture at a recommended ratio of 20µl of reagent to 100µl of culture medium. - [Read Cell Viability Assays that Measure Metabolic Capacity Protocol]

CellTiter-Glo Luminescent Cell Viability Assay Protocol - http://www.promega.com/tbs/tb288/tb288.pdf

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Protocol for CellTiter-Glo luminescent cell viability assay. This assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells. - [Read CellTiter-Glo Luminescent Cell Viability Assay Protocol]

Chambers for Examination of Live Cells under Mechanical Stress Protocol - http://www.cshprotocols.org/cgi/content/extract/2007/3/pdb.ip32

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Manual measurement and manipulation of the cell surface requires access to the cells, usually in an open chamber. Temperature-controlled chambers or stage inserts are preferred for maintaining physiological activity during the experiment. For example, heated culture dishes with coverslip glass bottoms (Bioptechs) permit high-resolution fluorescence microscopy of living cells during force application. - [Read Chambers for Examination of Live Cells under Mechanical Stress Protocol]

ChIP Assay Protocol PDF - http://www.bcm.edu/rosenlab/protocols/ChIP.pdf

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Formaldehyde cross-linking and chromatin immunoprecipitation assays of tissue culture cells, Based on Boyd and Farnham. Michelle Kallesen, Rosen Lab. - [Read ChIP Assay Protocol PDF]

Conditioned Medium for Retinal Explant Culture Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C6.html

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: Neuronal Cell Culture Media Protocols

Protocol for conditioned medium for retinal explant culture. - [Read Conditioned Medium for Retinal Explant Culture Protocol]

Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4350

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

Protocol provides methods for cryofreezing and subsequent thawing of mammalian cells. Pre-confluent cells are trypsinized, pelleted, resuspended in freezing medium, and gradually frozen. When needed, frozen cells are thawed quickly under running tap water and transferred to growth medium. - [Read Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples Protocol]

Culture and Characterization of Human Embryonic Stem Cells. PDF - http://www.liebertonline.com/doi/pdf/10.1089/scd.2004.13.325

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Culture and Characterization of Human Embryonic Stem Cells. DRAPER et al., STEM CELLS AND DEVELOPMENT 13:325Ã336 (2004) - [Read Culture and Characterization of Human Embryonic Stem Cells. PDF]

Culture and Expansion of OT-1 TCR Transgenic T cells Protocol - http://www.natureprotocols.com/2007/01/29/culture_and_expansion_of_ot1_t.php

Category: Immunology: T Cell Protocols

Protocol for culture and expansion of OT-1 TCR transgenic T cells. - [Read Culture and Expansion of OT-1 TCR Transgenic T cells Protocol]

Culture Chambers for Live-Cell Imaging - http://www.microscopyu.com/articles/livecellimaging/culturechambers.html

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Specimen chambers have had many designs published over the years describing systems that offer excellent optical properties while allowing specimens to be maintained for varying amounts of time. Ranging in complexity from the simple preparation of a sealed coverslip on a microscope slide to sophisticated perfusion chambers that enable tight control of virtually all environmental variables culture chambers are designed to to allow living specimens to be observed with minimal invasion at high res. - [Read Culture Chambers for Live-Cell Imaging]

Culture Conditions for Various Stable Cell Lines - http://www.flemingtonlab.com/Protocols/CultureStableCellLines.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Culture conditions for various stable cell lines. - [Read Culture Conditions for Various Stable Cell Lines]

Culture of BEND Cells (Bovine Endometrial Cells) Protocol - http://www.animal.ufl.edu/hansen/protocols/bend.htm

Category: Cell Culture Protocols

Protocol for culture of BEND cells (Bovine Endometrial Cells). Includes: Culture medium, Thawing Cells, Trypsinazation/ Splitting and freezing. - [Read Culture of BEND Cells (Bovine Endometrial Cells) Protocol]

Culture of Dissociated Cortical Neurons Protocol - http://crutcherlab.med.uc.edu/DCN.htm

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Protocol for culture of dissociated cortical neurons. - [Read Culture of Dissociated Cortical Neurons Protocol]

Culture of Dissociated Sympathetic Neurons Protocol - http://crutcherlab.med.uc.edu/DSN.htm

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Protocol for culture of dissociated sympathetic neurons. - [Read Culture of Dissociated Sympathetic Neurons Protocol]

Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion - http://www.animal.ufl.edu/hansen/protocols/endocult.htm

Category: Cell Culture Protocols

Protocol used to study secretion of proteins and prostaglandins by endometrium from the cow, ewe, mare, bitch and other species. The technique is also useful for culture of peri-implantation conceptuses and placental tissues for metabolic labelling studies and to obtain conceptus secretory proteins for biological studies.The medium used is called Pig MEM, which is a modified minimum essential medium supplemented with non-essential amino acids, vitamins, insulin and additional glucose. - [Read Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion]

Culture of Human Embryonic Stem Cells (hESC) - http://stemcells.nih.gov/research/NIHresearch/scunit/culture.asp

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Culture of Human Embryonic Stem Cells (hESC). one simple protocol outlined. NIH Stem Cell Unit - [Read Culture of Human Embryonic Stem Cells (hESC)]

Culture of Human Embryonic Stem Cells (hESC) Protocol - http://stemcells.nih.gov/research/NIHresearch/scunit/culture.asp

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Protocol for the culture of human embryonic stem cells (hESC). - [Read Culture of Human Embryonic Stem Cells (hESC) Protocol]

Culture of Human Prostatic Carcinoma Cell Lines - http://www.celldeath.de/apometh/rpmi.html

Category: Cell Biology and Cell Culture: Cell Line Culture Conditions

Culture of Human Prostatic Carcinoma Cell Lines, Growing and splitting the cells, Preparation of frozen stocks in liquid nitrogen, How to bring frozen cells back into culture, Concentrations of antibiotics for the selection of stable transfectants. LNCa - [Read Culture of Human Prostatic Carcinoma Cell Lines]

Culture Protocols and Information for T brucei - http://tryps.rockefeller.edu/crosslab_protocol_culture.html

Category: Bacterial Protocols

Culture protocols and information for T brucei. Detailed guide to the practice and history ofTrypanosoma brucei cultivation. This covers the culture of bloodstream and procyclic forms in liquid media and on agarose plates. - [Read Culture Protocols and Information for T brucei]

Culturing Marine Euplotids Using Dunaliella as a Food Source Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4467

Category: Laboratory Model Organisms Protocols

Protocol describes the culture of marine euplotids using Dunaliella salina or D. tetiolecta as a food organism. Dunaliella tolerate a wide range of salinity, thus they are fairly easy to grow in the lab using artificial sea salts. - [Read Culturing Marine Euplotids Using Dunaliella as a Food Source Protocol]

Culturing Trophoblast Stem (TS) Cell Lines Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4406

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Culture conditions have been established for a second blastocyst-derived cell line, trophoblast stem (TS) cells, in addition to embryonic stem (ES) cells. This protocol describes a method for culturing TS cell lines. These cells can then be used to study trophoblast differentiation and placental function. - [Read Culturing Trophoblast Stem (TS) Cell Lines Protocol]

Articles (1-7 of 7)

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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