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Acid Cleaning Coverslips Protocol - http://spectorlab.cshl.edu/acid_clean.html

Category: Immunohistochemistry Protocols: Coated Treated Slides Protocols

Protocol for acid cleaning coverslips. - [Read Acid Cleaning Coverslips Protocol]

Analysis of the Plant Epidermis Experiments - http://www.arabidopsis.org/cshl-course/6-epidermis.html

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Experiments included for analysis of plant epidermis: Steroid induction of gene expression in Arabidopsis; Replica Molds and Casts of the plant epidermis. - [Read Analysis of the Plant Epidermis Experiments]

Fluorescence Mounting Medium (Antifade) - http://spectorlab.cshl.edu/fluorescence_medium.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Fluorescence Mounting Medium (Antifade). Spector Lab, CSHL. - [Read Fluorescence Mounting Medium (Antifade)]

Fluorescence Mounting Medium (Antifade) Protocol - http://spectorlab.cshl.edu/fluorescence_medium.html

Category: Immunology: Immunofluorescence Protocols

Protocol for fluorescence mounting medium (antifade). - [Read Fluorescence Mounting Medium (Antifade) Protocol]

Gene Expression and Protein Localization in Arabidopsis Protocols - http://www.arabidopsis.org/cshl-course/7-gene_expression.html

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocols for gene expression and protein localization in Arabidopsis. Includes: Detection of the native protein; Detection of a recombinant version; Immunofluorescence detection in Arabidopsis protoplasts; Isolation of Arabidopsis seedling protoplasts; Subcellular localization of GUS-fusion proteins in Arabidopsis seedlings; Localization of Arabidopsis proteins with GUS in situ enzyme assay. - [Read Gene Expression and Protein Localization in Arabidopsis Protocols]

Genetic Mapping of Arabidopsis Centromeres Using PCR-Based Markers Protocol - http://www.arabidopsis.org/cshl-course/3-genetic_mapping.html

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for genetic mapping of Arabidopsis centromeres using PCR-based markers. Includes: DNA markers facilitate genome analysis; Centromere mapping using tetrad analysis; Preparation of DNA from frozen plant tissue. - [Read Genetic Mapping of Arabidopsis Centromeres Using PCR-Based Markers Protocol]

Immunofluroescence Technique Protocol - http://spectorlab.cshl.edu/immunofluorescence.html

Category: Immunology: Immunofluorescence Protocols

Protocol for immunofluroescence technique. - [Read Immunofluroescence Technique Protocol]

Isolation of Arabidopsis cDNAs by Complementation in Yeast - http://www.arabidopsis.org/cshl-course/12-yeast.html

Category: Plant Biology Protocols: Arabidopsis Nucleic Acid Protocols

Includes: Isolation of Arabidopsis LEU2 cDNAs by complementation of the yeast leu2 mutation; Recovery of plasmid DNA from yeast cells; Analysis of Arabidopsis cDNAs that complement a yeast leu2 mutation; Preparation of library DNA from the Lacroute cDNA library; Preparation of Yeast Media. - [Read Isolation of Arabidopsis cDNAs by Complementation in Yeast]

mRNA In Situ Hybridization with Nick-Translated Probes Protocol - http://spectorlab.cshl.edu/in_situ_hybridization.html

Category: Cytogenetics Protocols: RNA In Situ Hybridization Protocols

Protocol for mRNA in situ hybridization with nick-translated probes. - [Read mRNA In Situ Hybridization with Nick-Translated Probes Protocol]

Post-Embedding Immunogold Method Protocol - http://spectorlab.cshl.edu/immunogold_labeling.html

Category: Immunohistochemistry Protocols

Protocol for post-embedding immunogold method. - [Read Post-Embedding Immunogold Method Protocol]

Ubiquitin Assay Protocol for Yeast - http://tanseylab.cshl.edu/pdf/His_Ub_Yeast.pdf

Category: Protein Protocols: Protein Ubiquitination Protocols

Ubiquitin Assay Protocol for Yeast. Tansey Lab. - [Read Ubiquitin Assay Protocol for Yeast]

Ultimate Freeze Thaw Lysis For Mammalian Cells - http://tanseylab.cshl.edu/pdf/Freeze_Thaw_Lysis.pdf

Category: Protein Protocols: Protein Extraction From Cells

Ultimate Freeze Thaw Lysis For Mammalian Cells. Tansey lab. Cell harvesting. Includes recipes for solutions for freeze thawing lysis for cells. - [Read Ultimate Freeze Thaw Lysis For Mammalian Cells]

Ultimate His-UB Assay for Mammalian Cells - http://tanseylab.cshl.edu/pdf/His_Ub_mammals.pdf

Category: Protein Protocols: Protein Ubiquitination Protocols

Ultimate His-UB Assay for Mammalian Cells. Tansey Lab Protocols. William P. Tansey. PREPARATION OF NI-NTA-AGAROSE, HARVESTING THE TRANSFECTION., ELUTION OF HIS-TAGGED PROTEINS. - [Read Ultimate His-UB Assay for Mammalian Cells]