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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

b-galactosidase Solution Assay Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/B2.html

Category: Immunohistochemistry Protocols

Protocol for b-galactosidase solution assay. - [Read b-galactosidase Solution Assay Protocol]

BAC-FISH Protocol - http://www.nhm.ac.uk/hosted_sites/schisto/protocols/BAC_FISH.html

Category: BACs Bacterial Artificial Chromosome Protocols

Protocol uses the BIOPRIME reaction kit from GibcoBRL to prepare biotin-labelled BAC DNA which is detected using FITC-Avidin (Vector Labs, DCS grade). Reagents from other manufacturers may work equally well but have not been tested. Includes: Labeling of BAC clones; Ethanol precipitation; Hybridization; Post-hybridisation treatment / detection. - [Read BAC-FISH Protocol]

BACs Bacterial Artificial Chromosomes - http://openwetware.org/wiki/Bacterial_artificial_chromosomes

Category: BACs Bacterial Artificial Chromosome Protocols

Information for BACs, Bacterial Artificial Chromosomes. Includes: Existing vectors; pBAC108L; pBeloBAC11; pBACe3.6; pFW11 and F' factor; pCC1BAC; pSMART VC Vectors; pIndigoBAC-5; Cosmids; pWEB-TNCTM; SuperCos 1; pFos1; PAC vectors; BAC purification; Putting the BAC into E. coli. - [Read BACs Bacterial Artificial Chromosomes]

Bacteria-Mediated (Feeding) RNAi Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Feeding_RNAi.htm

Category: C. elegans Protocols

Protocol for bacteria- mediated (feeding) RNAi. - [Read Bacteria-Mediated (Feeding) RNAi Protocol]

Bacterial Cell Maintenance Protocol - http://www.genome.ou.edu/protocol_book/protocol_partI.html#I.G

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

4 strains of E. coli are used in these studies: JM101 for M13 infection and isolation, XL1BMRF'for M13 or pUC-based DNA transformation, and ED8767 for cosmid DNA transformation. To maintain their respective F' episomes necessary for M13 viral infection, JM101 is streaked onto a M9 minimal media plate and XL1BMRF' is streaked onto an LB plate containing tetracycline. ED8767 is streaked onto an LB plate. These plates are incubated at 37degC overnight. For each strain, 3 ml. of appropriate liquid.. - [Read Bacterial Cell Maintenance Protocol]

Bacterial clone DNA Protocol for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/DNAprep.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Bacterial clone DNA Protocol for FISH. - [Read Bacterial clone DNA Protocol for FISH]

Bacterial Colony PCR - http://www.cbs.umn.edu/~kclark/protocols/bactPCR.html

Category: PCR Protocols: Colony PCR

Bacterial Colony PCR. Karl Clark, University of Minnesota. - [Read Bacterial Colony PCR]

Bacterial Glycerol Stocks Protocol - http://www.unc.edu/depts/van_dyke/protocols/bacteria/bacterial_glycerol_stocks.pdf

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

To 2mls of mid-log culture or 1ml of freshly saturated culture add 1ml(or an equal volume) of glycerol solution, mix gently, then freeze rapidly in liquid nitrogen or on dry ice. Store at -20°C and - 70°C. - [Read Bacterial Glycerol Stocks Protocol]

Bacterial Media Solutions and Stocks - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid11.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

agar, Ampicillin Stock, Alkaline Lysis Solution, SOB, SOC media, and X-gal stock solutions. Donis Keller. - [Read Bacterial Media Solutions and Stocks]

Bacterial Solutions - http://www.uhmc.sunysb.edu/bioscience/methods/bacteria_methods/bacteria_methods.htm

Category: Buffers Media, and Solutions: Bacterial Solutions

Bacterial Solutions. Stony Brook Univ. Hospital. - [Read Bacterial Solutions]

Bacterial Transformation Electroporation Protocol - http://rothlab.ucdavis.edu/protocols/T10.html

Category: Transfection Protocols: Electroporation Sonoporation Protocols

Protocol for bacterial transformation electroporation. Includes: Preparation of Electrocompetent cells. - [Read Bacterial Transformation Electroporation Protocol]

Bacteriophage Lamda Plant Genomic Library Construction Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/GenomicDNAPhageLibraryConstruction.pdf

Category: DNA Protocols: Genomic DNA Library Protocols

Protocol for bacteriophage lamda plant genomic library construction. Includes: Preparation of the insert DNA; Pilot Sau3A Digestion; Full-scale partial digestions. - [Read Bacteriophage Lamda Plant Genomic Library Construction Protocol]

Baculovirally Infected Sf9 Cell Lysis Protocol - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/Sf9lysis.html

Category: Cloning Protocols: Baculovirus Protein Expression Protocols

Protocol for Baculovirally infected Sf9 cell lysis. Includes: Harvesting; Lysis. - [Read Baculovirally Infected Sf9 Cell Lysis Protocol]

Baculovirus Analysis of Nonsecreted Protein Expression Protocol - http://www.ebioscience.com/ebioscience/appls/BV.htm#B

Category: Cloning Protocols: Baculovirus Protein Expression Protocols

Protocol for Baculovirus analysis of nonsecreted protein expression. - [Read Baculovirus Analysis of Nonsecreted Protein Expression Protocol]

Baculovirus Cotransfection Protocol - http://www.ebioscience.com/ebioscience/appls/BV.htm#A

Category: Cloning Protocols: Baculovirus Protein Expression Protocols

Protocol for Baculovirus cotransfection. - [Read Baculovirus Cotransfection Protocol]

Baculovirus Growing and Freezing Insect Cells Protocol - http://www.ebioscience.com/ebioscience/appls/BV.htm#C

Category: Cloning Protocols: Baculovirus Protein Expression Protocols

Protocol for Baculovirus growing and freezing insect cells. - [Read Baculovirus Growing and Freezing Insect Cells Protocol]

Baculovirus Production for Recombinant Proteins - http://axon.med.harvard.edu/~cepko/protocol/mike/B1.html

Category: Protein Protocols: Protein Expression Protocols

Baculovirus Production for Recombinant Proteins. Protocol is a modified version of the pharmingen Baculogold kit. Brian Dynlacht. Mike A. Dyer Lab. - [Read Baculovirus Production for Recombinant Proteins]

Baculovirus Protein Expression Protocols - http://www.ebioscience.com/ebioscience/appls/BV.htm

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Baculovirus protein expression protocols. Includes protocols: Cotransfection Protocol; Analysis of nonsecreted protein expression; Growing and Freezing Insect Cells. - [Read Baculovirus Protein Expression Protocols]

Baculovirus Protein Expression Protocols - http://www.ebioscience.com/ebioscience/appls/BV.htm

Category: Cloning Protocols: Baculovirus Protein Expression Protocols

Protocols for Baculovirus protein expression. - [Read Baculovirus Protein Expression Protocols]

Balanced Salts for Growth Media Protocol - http://www.sigmaaldrich.com/img/assets/11020/HBS.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

Protocol for balanced salts used for growth media. - [Read Balanced Salts for Growth Media Protocol]

Band Shift Protocols - http://www.pharma.ethz.ch/institute_groups/biomacromolecules/protocols/bandshift

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocols on band shift. Includes: Phosphorylation of recombinant antibodies; Labeling the antigen with a red fluorophore; Native gel electrophoresis; Band-shift assay for the determination of Kd; Band-shift assay for the determination of koff. - [Read Band Shift Protocols]

Basic Cell Culture Protocols - http://www.hyclone.com/library/basicprotocols.htm

Category: Cell Biology and Cell Culture

Basic Cell Culture Protocols. Passaging, Counting, Viability and more. Hyclone - [Read Basic Cell Culture Protocols]

Basic ELISA Method - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/BasicELISAProtocol.htm

Category: ELISA Protocols

Basic ELISA Method. All the steps for the basic ELISA protocol. Immunology Applications Core Facility, Univ. Chicago. - [Read Basic ELISA Method]

Basic Ideas in PCR Protocol Design. PDF - http://www.idtdna.com/support/technical/TechnicalBulletinPDF/A_Basic_PCR_Protocol.pdf

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Basic Ideas in PCR Protocol Design. PDF - [Read Basic Ideas in PCR Protocol Design. PDF]

Basic Ideas in PCR Protocol Design. PDF - http://www.idtdna.com/support/technical/TechnicalBulletinPDF/A_Basic_PCR_Protocol.pdf

Category: PCR Protocols

Basic Ideas in PCR Protocol Design. PDF - [Read Basic Ideas in PCR Protocol Design. PDF]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.