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CsCl Prep of Plasmid DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C1.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for CsCl prep of plasmid DNA. This is a standard large scale prep. For plasmid DNA which gives a yield of 0.5 1.0 mg. - [Read CsCl Prep of Plasmid DNA Protocol]

DNA Extraction Procedure (CsCl) - Rhizobium - http://wheat.pw.usda.gov/~lazo/methods/lazo/rhizodna.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol describing extraction of DNA from Rhizobium. - [Read DNA Extraction Procedure (CsCl) - Rhizobium]

Purification of Bacteriophage lambda Particles by Isopycnic Centrifugation through CsCl Gradients - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3968

Category: Viral Protocols

Isopycnic centrifugation through CsCl gradients is used to prepare infectious bacteriophage {lambda} particles of the highest purity that are essentially free of contaminating bacterial nucleic acids. - [Read Purification of Bacteriophage lambda Particles by Isopycnic Centrifugation through CsCl Gradients]

Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradient - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3913

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

A solution containing plasmid DNA, saturating amounts of ethidium bromide, and CsCl (44% w/v) is layered between two solutions of lesser (35% w/v CsCl) and greater density (59% w/v CsCl). During centrifugation to equilibrium, the closed circular plasmid DNA and linear DNAs form bands at different densities. - [Read Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradient]

Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3927

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Solutions containing plasmid DNA are adjusted to a density of 1.55 g/ml with solid CsCl. The intercalating dye, ethidium bromide, which binds differentially to closed circular and linear DNAs, is then added to a concentration of 200 mu;g/ml. During centrifugation to equilibrium, the closed circular DNA and linear DNAs form bands at different densities. - [Read Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients]