cryostat Protocols

Category: Molecular Imaging: Immunofluorescence Microscopy

B or T cells in suspension, adherent cells on chambered coverglass or chamberslides, cryostat sections of unfixed, OCT embedded tissue. Susan Anderson. - [Read Immunofluorescence / confocal microscopy]

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Frozen tissue sections show good preservation of tissue structure and antigens. The principle disadvantages of using them in immunostaining are that the specimens must be stored frozen, and a special microtome, known as a cryostat, is required. Also, many clinical specimens are not available in this form, and most classic histological descriptions of tissue structure and pathology are based on the use of paraffin-embedded sections of formalin-fixed material. - [Read Preparing Frozen Tissue Sections for Immunostaining Protocol]

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for X-gal staining of Drosophila frozen cryostat sections. - [Read X-Gal Staining of Drosophila Frozen Cryostat Sections Protocol]