crosses Protocols

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How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab - http://www.fgsc.net/neurosporaprotocols/How%20to%20avoid%20pitfalls%20and%20keep%20out%20of%20trouble.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

How to Determine Gene Order Using 3-Point Crosses - http://www.fgsc.net/neurosporaprotocols/How%20to%20determine%20gene%20order%20using%203-point%20crosses%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to determine gene order using 3-point crosses. - [Read How to Determine Gene Order Using 3-Point Crosses]

How to Enhance the Formation of Perithecia and the Production of Progeny by Poorly Fertile Crosses - http://www.fgsc.net/neurosporaprotocols/How%20to%20enhance%20the%20formation%20of%20perithecia.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to enhance the formation of perithecia and the production of progeny by poorly fertile crosses. - [Read How to Enhance the Formation of Perithecia and the Production of Progeny by Poorly Fertile Crosses]

How to Establish Parentage in Reciprocal Crosses - http://www.fgsc.net/neurosporaprotocols/How%20to%20establish%20parentage%20%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to establish parentage in reciprocal crosses. - [Read How to Establish Parentage in Reciprocal Crosses]

How to Minimize “Bubble-Ascus” Abortion in Crosses for Cytology - http://www.fgsc.net/neurosporaprotocols/How%20to%20minimize%20bubble-ascus%20abortion%20in%20crosses%20for%20cytology.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to minimize “bubble-ascus” abortion in crosses for cytology. - [Read How to Minimize “Bubble-Ascus” Abortion in Crosses for Cytology]

How to Obtain Uncontaminated Progeny From Crosses Involving per-1 - http://www.fgsc.net/neurosporaprotocols/How%20to%20obtain%20uncontaminated%20pr%20edited.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to obtain uncontaminated progeny from crosses involving per-1. - [Read How to Obtain Uncontaminated Progeny From Crosses Involving per-1]

Neurospora Methods Protocols - http://www.fgsc.net/methods/stanford.html

Category: Fungi Protocols: Neurospora Protocols

Protocols for Neurospora methods. Includes: Standard strains; Crosses; Minimal medium; Color coding of media; Agar substrate for manipulation and isolation; Stock solutions of supplements; Mating-type tests; Preservation of stocks by silica gel; Cleaning of glassware; Control of mites. - [Read Neurospora Methods Protocols]

Plant Crosses Protocol - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/PlantWork/crosses.html

Category: Plant Biology Protocols: Plant Genetics Protocols

Protocol describing on how to perform crosses in plants. - [Read Plant Crosses Protocol]

Rapid Method for Making Large Numbers of Crosses Protocol - http://www.fgsc.net/neurosporaprotocols/Rapid__crossesDE.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol for a rapid method for making large numbers of crosses. - [Read Rapid Method for Making Large Numbers of Crosses Protocol]

Setting Up Arabidopsis Crosses Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4623

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Arabidopsis naturally self-pollinates, the generation of cross-progeny requires some intervention by the investigator. This protocol describes the generation and collection of seeds by crossing suitable Arabidopsis parent plants. - [Read Setting Up Arabidopsis Crosses Protocol]