creation Protocols

Category: Immunology: Chemotaxis

Chemotaxis Assay, Springer Lab. A chemotaxis assay's function is to assess whether a factor or molecule of interest has chemotactic activity on a motile cell type. Chemotaxis is the ability of a factor to cause the migration of a cell. The chemotactic assay is based on the creation of a chemical gradient of the chemotactic agent which will cause cells to migrate through the gradient towards the chemotactic agent. - [Read Chemotaxis Assay]

Category: DNA Protocols: EMSA DNA-Protein Protocols

EMSA probe creation using ds Oligonucleotides by annealing two complementary oligos, and Klenow for probe creation. - [Read EMSA using ds Oligonucleotides]

Category: Peptide Protocols: Antibodies from Peptides Protocols

Protocol for Antibody Creation from a Synthetic Peptide. Antibody from Peptides. Daniel O'Connor. - [Read Making antibodies to synthetic peptides]

Category: DNA Microarray Protocols

Heather R. Sanders. Hybridization, Probe Creation and RNA preparation for Microarray Protocols. - [Read Microarray Protocols]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Protocol Buffer Creation. Based on Jane Gitschier's group.Fred Hutchinson Cancer Research Center. - [Read PCR Protocol Buffer Creation]

Category: PCR Protocols

PCR Protocol Buffer Creation. Based on Jane Gitschier's group.Fred Hutchinson Cancer Research Center. - [Read PCR Protocol Buffer Creation]

Category: DNA Protocols: EMSA DNA-Protein Protocols

Creation of Probe for EMSA using Oligonucleotide annealing, p-32, and T4 Polynucleotide Kinase. - [Read Radiolabeling of Probes for Electrophoretic Mobility Shift Assay]