coverslips Protocols

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Acid Cleaning Coverslips Protocol - http://spectorlab.cshl.edu/acid_clean.html

Category: Immunohistochemistry Protocols: Coated Treated Slides Protocols

Protocol for acid cleaning coverslips. - [Read Acid Cleaning Coverslips Protocol]

Chrome Gelatinised Slides and Coverslips Protocol - http://www.nottingham.ac.uk/pathology/protocols/gelslid.html

Category: Immunohistochemistry Protocols: Coated Treated Slides Protocols

Protocol for chrome gelatinised slides and coverslips. - [Read Chrome Gelatinised Slides and Coverslips Protocol]

Growing Adherent Cells on Coverslips or Multiwell Slides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4289

Category: Cell Culture Protocols

Glass is an excellent substrate for most tissue-culture-adapted cells and is compatible with all fixing and staining solutions. Glass coverslips in tissue-culture dishes or in 24-well multiwell plates are suitable carriers, as are multiwell slides. For high-resolution studies, choose glass coverslips of the highest available grade; #1 or #1.5 coverslips are the appropriate thickness. - [Read Growing Adherent Cells on Coverslips or Multiwell Slides Protocol]

Immunohistochemistry using Anti-Ganglioside Antibodies - http://www.glycotech.com/protocols/Proto2.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Indirect method measuring immunofluorescence coupled to second antibody. Best for membrane antigens in addition to intra- and extracellular antigens, may be applied to frozen tissue sections, to cells in suspension, and to cells attached to glass slides or coverslips. Tadashi Tai~Head, Department of Tumor Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan - [Read Immunohistochemistry using Anti-Ganglioside Antibodies]

Immunostaining of Cells Adherent to Coverslips - http://www.people.virginia.edu/~gwl6s/protocols/cellstain.html

Category: Immunology: Immunocytochemistry Protocols

Laurie Lab, Univ. Virginia. - [Read Immunostaining of Cells Adherent to Coverslips]

Plating Cells for Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4650

Category: Cell Culture Protocols

Protocol describes a method for plating cells for microinjection onto etched coverslips. The coverslips for microinjection must be marked so that microinjected cells can be identified at time points after injection. - [Read Plating Cells for Microinjection Protocol]

Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. The calcium phosphate method can be used to transfect neurons that have already been growing on coverslips in vitro. Transfected cells suitable for imaging can be obtained in cultures up to 15 days in vitro. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid]