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"CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size - http://www.mcb.uct.ac.za//corepcr.htm

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

"CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size - Molecular Biology Techniques Manual - Rybicki - [Read "CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size]

"CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size - http://www.mcb.uct.ac.za//corepcr.htm

Category: PCR Protocols: Standard PCR Protocol

Annexin V Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/annexin.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Different cell types vary in their phosphatidylserine (PS) content, along with the amount of PS exposure on the cell surface after cell death. This protocol is a guideline for getting started, however it may be necessary to adjust the concentration of the Annexin V-FITC. - [Read Annexin V Protocol for Flow Cytometry]

Basic ELISA Method - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/BasicELISAProtocol.htm

Category: ELISA Protocols

Basic ELISA Method. All the steps for the basic ELISA protocol. Immunology Applications Core Facility, Univ. Chicago. - [Read Basic ELISA Method]

Calcium Flux Analysis Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/calcium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for calcium flux analysis. Includes: Reagent Preparation; Sample Preparation; Cell Surface Staining; Instrument Set up; Optimizing the Instrument Settings; Recording Data to Apply Compensation; Recording Experimental Data. - [Read Calcium Flux Analysis Protocol for Flow Cytometry]

Calcium Flux Analysis Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/calcium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Protocol for calcium flux analysis for flow cytometry. - [Read Calcium Flux Analysis Protocol for Flow Cytometry]

CaspaTag Caspase Activity Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/caspatag.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

This assay is based on carboxyfluorescein labeled fluoromethyl ketone (FMK)-peptide inhibitors of caspases. Includes: Working Dilution of FMK-peptide inhibitors; 1X Working Dilution Wash Buffer; Protocol for Flow Cytometry. - [Read CaspaTag Caspase Activity Protocol]

Cell Culture Guidelines - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/CellCultureGuidelines.htm

Category: Cell Biology and Cell Culture: Troubleshooting Cell Culture Problems

Immunology Applications Core Facility, Univ. Chicago - [Read Cell Culture Guidelines]

Cell Cycle Protocol using Flow Cytometry - http://www.people.cornell.edu/pages/jls269/protocols/cellcycleprotocols.html

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Cell Cycle Protocol using Flow Cytometry. Cell cycle Analysis by Flow Cytometry. DNA content Analysis on the FACSAria. Analyzing Cell cycle FCM data. Biomedical Sciences Flow Cytometry Core Laboratory. Dr.Smith Cornell. - [Read Cell Cycle Protocol using Flow Cytometry]

Cell Thawing and cell line Freezing Protocol - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/FreezeThawProtocol.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Method for freezing and thawing cells. Immunology Applications Core Facility. U. Chicago - [Read Cell Thawing and cell line Freezing Protocol]

DNA Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/dna.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for DNA analysis by flow cytometry. - [Read DNA Analysis by Flow Cytometry Protocol]

ES mouse cell stem cell Culture - http://imgen.bcm.tmc.edu/dtmc/mouse_embryonic_stem_cell_culture.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Mouse Embryonic Stem Cell Culture. Southern Analysis of ES cells. The Darwin Transgenic Core Facility - [Read ES mouse cell stem cell Culture]

FASC Analysis of Cell Cycle using BrdU and PI Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/fasc.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocols for FASC analysis of cell cycle using BrdU and PI. Includes: Labeling of Cells with BrdU; Collecting the Cells; Processing the Cells. - [Read FASC Analysis of Cell Cycle using BrdU and PI Protocol]

Genotyping Transgenic Rodents by PCR - http://www.med.umich.edu/tamc/pcr.html

Category: Transgenic Mouse Protocols: Mouse Genotyping Protocols

Test mice and rats for the presence of the transgene by PCR. University of Michigan, Transgenic Animal Model Core - [Read Genotyping Transgenic Rodents by PCR]

Immunofluorescent Staining of Rat Thymocytes Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/immunofluorescent.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for the immunofluorescent staining of rat thymocytes. - [Read Immunofluorescent Staining of Rat Thymocytes Protocol]

In vitro Translation Assays for CFTR - http://pen2.igc.gulbenkian.pt/cftr/vr/c/ostedgaard_in_vitro_translation_assays_for_cftr.pdf

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol outlines the general procedure and requirements for in vitro translation of CFTR and outlines some assays using in vitro translated product. Core glycosylation of CFTR occurs in the ER. An assay for this processing step requires the addition of microsomal membranes to the basic in vitro translation mixture. This protocol takes this into account. - [Read In vitro Translation Assays for CFTR]

In-gel Digestion protocol - http://www.hmc.psu.edu/core/oldsite/Maldi/malditofprotocols.htm

Category: Proteomic Protocols

In-gel Digestion protocol. BA Stanley. - [Read In-gel Digestion protocol]

Isolation of DNA from Mouse Tail Biopsies - http://www.med.umich.edu/tamc/tDNA.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Isolation of DNA from Mouse Tail Biopsies. University of Michigan Transgenic Animal Model Core, Transgenic Animal Web. - [Read Isolation of DNA from Mouse Tail Biopsies]

Membrane Potential Analysis by Flow Cytometry Protoocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/membrane.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for membrane potential analysis by flow cytometry. Includes: Preparation of the dyes; Loading of the dyes; Flow Cytometric Analysis. - [Read Membrane Potential Analysis by Flow Cytometry Protoocol]

Microinjection of Mouse ES Cells into Blastocysts - http://www.bcm.edu/dtmc/?pmid=7791

Category: Microinjection Protocols: Microinjection of DNA Protocols

The core defines one injection as a total of forty blastocysts injected on two consecutive days using one or two clones for the same mutation. Please, refer to the Pricing page for information on the cost of one injection. - [Read Microinjection of Mouse ES Cells into Blastocysts]

Other MALDI-TOF Sample Prep Protocols - http://www.hmc.psu.edu/core/oldsite/Maldi/othermalditofprotocols.htm

Category: Proteomic Protocols

Other MALDI-TOF Sample Prep Protocols. BA Stanley. - [Read Other MALDI-TOF Sample Prep Protocols]

PARP PE Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/parp.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for PARP PE. - [Read PARP PE Protocol]

Preparation of a Highly Efficient Transcription Extract from Drosophila Embryos Protocol - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D3APITJJIWFIM1R3FQLMCFEWHUWBNSIV0?id=p9052

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol describes how to produce a soluble nuclear extract rich in basal pol II transcription factors from Drosophila embryos. This is a cell-free extract that contains all the necessary transcription factors and is capable of accurate initiation of transcription by RNA polymerase II but is deficient in core histones and histone H1. - [Read Preparation of a Highly Efficient Transcription Extract from Drosophila Embryos Protocol]

Protocol for Manual Peptide Synthesis - http://www.biotech.unl.edu/proteomics/protocol.html

Category: Peptide Protocols

Protocol for Manual Peptide Synthesis. Resin Swelling And Coupling Of Activated Amino Acid Esters. Deblocking and cleaving peptide from solid support. Synthesis of Peptides. The University of Nebraska-Lincoln Protein Core Facility - [Read Protocol for Manual Peptide Synthesis]

Simple In-gel Digestion Protocol - http://www.hmc.psu.edu/core/oldsite/Maldi/ingelCPiPS.htm

Category: Proteomic Protocols

In-gel Digestion Protocol. BA Stanley. - [Read Simple In-gel Digestion Protocol]