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Competitive RT-PCR: Estimation of Copy Number Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4111

Category: PCR Protocols: Reverse Transcriptase RT-PCR Protocols

In this protocol, sample and competitor RNAs are reverse transcribed (separately) in a pilot experiment. A constant amount of sample RT product is then combined with a 2-logserial dilution of competitor RT product for PCR. Procedure provides an approximate copy number for the sample, which is then fine-tuned by repeating the experiment with a series of twofold dilutions of competitor. The experiment includes controls for sample-to-sample variations in RT efficiency. - [Read Competitive RT-PCR: Estimation of Copy Number Protocol]

Enzymatic Protocol for Immunocytochemistry - http://www.rndsystems.com/ihc_detail_objectname_EnzymaticProtocol20.aspx

Category: Immunology: Immunocytochemistry Protocols

Staining Tissue, Staining Cells Smeared on Slides. Positive staining controls, Negative staining controls, Immunoenzymatic Technique and FAQ. R & D Systems. - [Read Enzymatic Protocol for Immunocytochemistry]

General Method for Ras, Rac, Cdc42, and Rho Activation Assay - http://www.upstate.com/misc/protocol_detail.q.prot.e.general_method_ras.a.name.e.General_Method_for_Ras_Rac_Cdc42_and_Rho_Activation_Assay

Category: Signalling Signal Transduction Protocols

General protocol for Ras, Rac, Cdc42, and Rho activation assay. Includes: Affinity Precipitation/Immunoblot Protocol, Cell Culture and Extract Preparation (Adherent and Non Adherent cells), GTPÎ³S/GDP Loading for Positive and Negative Controls, Ras, Rac ,Cdc42, and Rho Pull-Down Assay and Western Blot and Detection. - [Read General Method for Ras, Rac, Cdc42, and Rho Activation Assay]

Processing Adherent Cultured Cells for Tissue Microarray Controls ... - http://www.tissuearray.org/CellLinesProtocolforTMA112.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Cell Fixation. 1. Gently wash cells 2x in sterile 1XPBS, ... [Take to Research Histology for embedding] Instructions: Embed pellets in vertical ... Jena Giltnane Yale University Dept. of Pathology. Processing Adherent Cultured Cells for Tissue Microarray Controls. - [Read Processing Adherent Cultured Cells for Tissue Microarray Controls ...]

Protein Coimmunoprecipitation in Arabidopsis Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4683

Category: Plant Biology Protocols: Arabidopsis Protein Peptide Protocols

Coimmunoprecipitation is one of the most useful techniques for revealing protein-protein interactions. Good negative controls to verify the specificity of the coimmunoprecipitation procedure are (1) performing the same immunoprecipitation experiment using beads coupled to the preimmune serum and (2) probing the Western blot with antibodies against protein known not to interact with the coimmunoprecipitated proteins under physiological conditions. - [Read Protein Coimmunoprecipitation in Arabidopsis Protocol]

Protocol Primary Mammalian Endothelial Cells - http://www.amaxa.com/fileadmin/groups/marketing/Downloads/Protocols/Primary_cells/amaxa_OP_Basic_Endothelial_VPI-1001.pdf

Category: Cell Biology and Cell Culture: Endothelial Cell Protocols

Primary mammalian endothelial cells protocol. This protocol is designed for primary endothelial cells isolated from various organs of mammals. Large and flat cells, often with large nuclei. Includes: Required reagents; DNA preparation and quality; Preparation of cells and cell culture; Important controls; Nucleofection protocol. - [Read Protocol Primary Mammalian Endothelial Cells]