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Yeast RNA Isolation: Small-Scale Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4155

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Protocol describes rapid, small-scale yeast RNA isolation. It is based on the work of Schmitt et al. (1990). Note that all containers should be washed in Rnase Away (Invitrogen) or dry baked for 24 hours at 160°C. - [Read Yeast RNA Isolation: Small-Scale Protocol]

Yeast Two-hybrid Systems Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3889

Category: Yeast Protocols: Yeast Two-Hybrid Systems Protocols

Protocol for Yeast two-hybrid systems. - [Read Yeast Two-hybrid Systems Protocol]

Yeast Vital Stains: Calcofluor Staining of Chitin and Bud Scars Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4166

Category: Yeast Protocols: Yeast Staining Protocols

Various vital stains are available for yeast. This protocol describes the staining of chitin and bud. - [Read Yeast Vital Stains: Calcofluor Staining of Chitin and Bud Scars Protocol]

Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4163

Category: Yeast Protocols: Yeast Staining Protocols

Protocol describes the staining of nuclear and mitochondrial DNA with DAPI (4',6-diamidino-2-phenylindole). - [Read Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol]

Yeast Vital Stains: Visualizing Mitochondria with DiOC6 or DiIC5 Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4164

Category: Cell Organelle Protocols: Mitochondria Protocols

Protocol describes the visualization of mitochondria with DiOC6 or DiIC5(3). - [Read Yeast Vital Stains: Visualizing Mitochondria with DiOC6 or DiIC5 Protocol]

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In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

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