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Protein isolation with tissue array construction - http://147.52.72.117/ijmm/2003/volume12/number3/299.pdf

Category: Protein Protocols: Protein Extraction From Tissue

Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes - [Read Protein isolation with tissue array construction]

Protocols for microarray development in use in the Vodkin Laboratory - http://soybeangenomics.cropsci.uiuc.edu/protocols/index.html

Category: DNA Microarray Protocols

Reverse transcriptase labeling with Cy3/Cy5, Staining of total DNA on slides, construction, Laser scanning, Spot quantitation, Statistics, sample tracking. Vodkin Laboratory. U.Illinois. - [Read Protocols for microarray development in use in the Vodkin Laboratory]

Replication Timing Using Transient Hemimethylation Protocol - http://fangman-brewer.genetics.washington.edu/hemimethylation.html

Category: Yeast Protocols: Yeast Genetics Protocols: Yeast Replication Protocols

Protocol for replication timing using transient hemimethylation. Includes: Strain construction; Protocol for methylation timing; Quantitation. - [Read Replication Timing Using Transient Hemimethylation Protocol]

Ribocloning: DNA Cloning and Gene Construction Using PCR Primers Terminated with a Ribonucleotide - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4142

Category: PCR Protocols: PCR Cloning Protocols

Protocol exploits the discovery that Rnase A can efficiently cleave at single rC or rU bases embedded in double-stranded DNA. Entire plasmid vectors are amplified using long, high-fidelity PCR with riboprimers, which carry a single rC residue at their 3' end. Target DNA is amplified using similar primers, which also end in a rC residue. - [Read Ribocloning: DNA Cloning and Gene Construction Using PCR Primers Terminated with a Ribonucleotide]

RNA Isolation for cDNA Library Construction Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/RNA%20Isolation.pdf

Category: DNA Protocols: cDNA Library Protocols

Protocol for RNA isolation for cDNA library construction. - [Read RNA Isolation for cDNA Library Construction Protocol]

Shotgun Library Construction Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/ShotgunLibraryConstruction%28EJSVersion-050511%29.pdf

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Protocol for shotgun library construction. - [Read Shotgun Library Construction Protocol]

Synthesis of First-strand cDNA Catalyzed by Reverse Transcriptase for Construction of cDNA Libraries - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4065

Category: DNA Protocols: cDNA Library Protocols

Protocol for the construction of construction of cDNA libraries by synthesis of first-strand cDNA catalyzed by reverse transcriptase. - [Read Synthesis of First-strand cDNA Catalyzed by Reverse Transcriptase for Construction of cDNA Libraries]

Systematic Mutagenesis of E.coli K-12 MG1655 ORFs Protocol - http://www.genome.wisc.edu/functional/tnmutagenesis.htm

Category: E Coli Protocols: E coli Genetics Protocols

Protocol for systematic mutagenesis of E.coli K-12 MG1655 ORFs. Includes: Mutagenesis Project Progress; Construction of Sce-poson mutants; Mutagenesis of Essential Genes; Gene Gorging. - [Read Systematic Mutagenesis of E.coli K-12 MG1655 ORFs Protocol]