conjugate Protocols

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ELISA Method - http://mycoplasmas.vm.iastate.edu/lab_site/methods/immunol/ELISA.html

Reagents, protocol and Conjugate Preparation. Minion Lab, College of Veterinary Medicine, Iowa State. - [Read ELISA Method]

Phycoerythrin Conjugation Protocol - http://pingu.salk.edu/flow/protocols/pelabel.html

Category: Immunology

Protocol for phycoerythrin conjugation. Includes: Preparation of PE; SPDP modification of PE; SMCC modification of antibody; DTT treatment of SPDP-PE; Purification of reactants; Conjugation; Stop reaction; Concentrate product; Separate conjugate. - [Read Phycoerythrin Conjugation Protocol]

Preparation Of Peptide-KLH Conjugates For Immunization - http://mitchison.med.harvard.edu/protocols/ab1.html

Category: Peptide Protocols: Antibodies from Peptides Protocols

Preparation Of Peptide-KLH Conjugates For Immunization. Coupling of Peptide to KLH. And generation of Antibodies using peptide-KLH conjugate - Claire Walczak. - [Read Preparation Of Peptide-KLH Conjugates For Immunization]

Protocol Immunoprecipitation - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Antibody_Explorer/Procedures/Immunoprecipitation.html

Category: Immunology: Immunoprecipitation Protocols

Immunoprecopitation method, the protein from the cell or tissue homogenate is precipitated in an appropriate lysis buffer by means of an immune complex which includes the antigen (protein), primary antibody and Protein A-, G-, or L-agarose conjugate or a secondary antibody-agarose conjugate - [Read Protocol Immunoprecipitation]