conformational Protocols

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Detection of Mutations by Single-strand Conformational Polymorphism Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3815

Category: Genetics and Genomics: Cancer Genetics Protocols: Single Strand Conformational Polymorphism(SSCP) Protocols

Single-strand conformational polymorphism (SSCP), one of several methods used to scan segments of DNA for mutations, exploits the electrophoretic differences in mobilities between single-stranded mutant and wild-type DNAs. - [Read Detection of Mutations by Single-strand Conformational Polymorphism Protocol]

Epitope Mapping by Competition Assay Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4277

Category: Antibody Protocols: Epitope Mapping Antibody Protocols

The simplest way to determine whether two monoclonal antibodies bind to distinct sites on a protein antigen is to carry out a competition assay. The assay can be used with antibodies that bind both conformational and linear epitopes, and it is most useful in the analysis of monoclonal antibody specificity because polyclonal sera typically recognize multiple different epitopes. - [Read Epitope Mapping by Competition Assay Protocol]

Mutation Detection By Single-Strand Conformational Polymorphism (SSCP) Protocol - http://www.ohsu.edu/nsi/faculty/reddyh/lab/protsscp.html

Category: Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols

Protocol for mutation detection by single-strand conformational polymorphism (SSCP). Includes: PCR Protocol; SSCP Gels; - [Read Mutation Detection By Single-Strand Conformational Polymorphism (SSCP) Protocol]

Mutation Detection By Single-Strand Conformational Polymorphism (SSCP) Protocol - http://www.ohsu.edu/nsi/faculty/reddyh/lab/protsscp.html

Category: Genetics and Genomics: Cancer Genetics Protocols: Single Strand Conformational Polymorphism(SSCP) Protocols

Protocol for mutation detection by single-strand conformational polymorphism (SSCP). Includes: PCR Protocol; SSCP Gels. - [Read Mutation Detection By Single-Strand Conformational Polymorphism (SSCP) Protocol]

Single-Strand Conformation Polymorphism Analysis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4118

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

The target sequence is simultaneously labeled and amplified, then heat-denatured and resolved by non-denaturing polyacrylamide gel electrophoresis. Differences in sequence alter the conformation of the DNA and hence its electrophoretic mobility and, because of the high resolution of polyacrylamide gels, most conformational changes caused by subtle changes in sequence can be detected. - [Read Single-Strand Conformation Polymorphism Analysis Protocol]