confocal Protocols

Category: Microscopy Protocols: In Vivo Imaging Protocols

To image early cleavages and chromatin dynamics, it is convenient to use histone H2B fused to GFP or lamin::GFP. Time-lapse movies can be obtained using conventional confocal microscope systems and their included software. Early embryos dissected from transgenic hermaphrodites are placed with egg salts on agar pads. Chromatin dynamics can be followed easily, and wild-type embryonic cells can be compared with mutants or RNAi-treated embryos. - [Read Protocol Live Imaging of Caenorhabditis Elegans]

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for performing wholemount fluorescent in situs. Instructions for two-color FISH and for a combination of fluorescent and colorimetric in situs we have called FCIS. Data can be imaged with a fluorescence stereoscope or a confocal. - [Read Xenopus Fluorescent in situs and FCIS Protocol]

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Zeiss Meta Confocal Microscopy Imaging Instructions. Operating Instructions with photos included. Keck Imaging Center. - [Read Zeiss Meta Confocal Microscopy Imaging Instructions]