confirmation Protocols

Search results for: confirmation

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

96-Well Confirmation Yeast PCR Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/96_well_confirmation.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for 96-well confirmation Yeast PCR. Includes: Clonal purification; Generate a master plate (96-well format); Making a frozen backup stock; Confirmation PCR for one Row; ORF Specific Confirmation PCR --> "A-B" primers (upstream junction); Transfer template DNA to multiwell PCR plate; Prepare and dispense master mix for A-B PCR. - [Read 96-Well Confirmation Yeast PCR Protocol]

Single tube confirmation PCR protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Single tube confirmation PCR protocol. Saccharomyces Genome Deletion Project. - [Read Single tube confirmation PCR protocol]

Single tube confirmation PCR protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: PCR Protocols

Single tube confirmation PCR protocol. Saccharomyces Genome Deletion Project. - [Read Single tube confirmation PCR protocol]

Single tube confirmation PCR protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: PCR Protocols: Colony PCR

The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformation along with a wild-type control. A series of five different PCR tests are performed on each colony to - [Read Single tube confirmation PCR protocol]

Single Tube Confirmation Yeast PCR Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for single tube confirmation yeast PCR. Includes: Clonal purification; Zymolyase treatment; PCR reactions; PCR conditions; Agarose gel electrophoresis. - [Read Single Tube Confirmation Yeast PCR Protocol]

Single-Strand Conformation Polymorphism Analysis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4118

Category: Genetics and Genomics: Cancer Genetics Protocols: Single Strand Conformational Polymorphism(SSCP) Protocols

Single-strand confirmation polymorphism analysis (SSCP) is a powerful and robust method for the detection of DNA sequence changes (single-base substitutions) based on shifts in electrophoretic mobility. In this protocol, the target sequence is simultaneously labeled and amplified, then heat-denatured and resolved by non-denaturing polyacrylamide gel electrophoresis. - [Read Single-Strand Conformation Polymorphism Analysis Protocol]

Viral Detection and Concentration in Food - http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf

Category: Viral Protocols

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods. - [Read Viral Detection and Concentration in Food]