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Determining Number of Live and Dead Cells in Cell Population: Cytotoxicity Assay Protocol - http://www.promega.com/paguide/chap4.htm#title4

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Cell-based assays are important tools for contemporary biology and drug discovery because of their predictive potential for in vivo applications.This assay gives ratiometric, inversely proportional values of viability and cytotoxicity (Figure 4.15) that are useful for normalizing data to cell number. Also, this reagent is compatible with additional fluorescent and luminescent chemistries. - [Read Determining Number of Live and Dead Cells in Cell Population: Cytotoxicity Assay Protocol]

Directional Cloning into Plasmid Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3919

Category: Cloning Protocols: Vector Protocols

Directional cloning requires that the plasmid vector be cleaved with two restriction enzymes that generate incompatible termini and that the fragment of DNA to be cloned carries termini that are compatible with those of the doubly cleaved vector. - [Read Directional Cloning into Plasmid Vectors Protocol]

DNA Affinity Chromatography Using Gravity Flow - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4206

Category: Chromatography Protocols: DNA RNA Affinity Chromatography

DNA Affinity Chromatography, DNA affinity chromatography can be a low-tech method using gravity flow at 4Â°C, a disposable chromatography column, and DNA affinity resin prepared in the laboratory (see Preparation of a DNA Affinity Column). Include 10-20% glycerol and 0.025-0.1% NP-40 in the column buffers to suppress losses due to nonspecific adsorption of protein to surfaces. Load the protein in a buffer that is compatible with binding of the protein to its target site. Keith Brocklehurst et al - [Read DNA Affinity Chromatography Using Gravity Flow - Subscription Required]

Gateway-Compatible Yeast One-Hybrid Screens Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4590

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Protocol for gateway-compatible yeast one-hybrid screens. - [Read Gateway-Compatible Yeast One-Hybrid Screens Protocol]

Growing Adherent Cells on Coverslips or Multiwell Slides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4289

Category: Cell Culture Protocols

Glass is an excellent substrate for most tissue-culture-adapted cells and is compatible with all fixing and staining solutions. Glass coverslips in tissue-culture dishes or in 24-well multiwell plates are suitable carriers, as are multiwell slides. For high-resolution studies, choose glass coverslips of the highest available grade; #1 or #1.5 coverslips are the appropriate thickness. - [Read Growing Adherent Cells on Coverslips or Multiwell Slides Protocol]

Identification of Positive GATEWAY Expression Clones Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4647

Category: Bacteria Genetics Protocols

Protocol for the identification of positive GATEWAY expression clones when both the pENTRY and pDEST vectors contain the same marker for bacterial selection. Protocol describes ways in which difficult vector combinations can be used effectively to obtain the appropriate expression clone without having to convert the pENTRY clone or pDEST clone to vectors with compatible antibiotic resistances. - [Read Identification of Positive GATEWAY Expression Clones Protocol]

Mass spectrometry compatible silver staining of gels protocol - http://www.narrador.embl-heidelberg.de/GroupPages/PageLink/activities/protocols/silverstain.html

Category: Mass Spectrometry Protocols

Mass spectrometry compatible silver staining of gels protocol. EMBL Bioanalytical Research Group. Wilm. - [Read Mass spectrometry compatible silver staining of gels protocol]

Mounting Samples in DPX Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4460

Category: Cell Culture Protocols: Cell Fixing Protocols

Mounting media for immunohistology must be compatible with the detection method used. A suitable non-aqueous mounting medium is DPX. - [Read Mounting Samples in DPX Protocol]

Mounting Samples in Gelvatol or Mowiol Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4461

Category: Cell Culture Protocols: Cell Fixing Protocols

Mounting media for immunohistology must be compatible with the detection method used. Suitable aqueous media are made from Gelvatol or Mowiol. - [Read Mounting Samples in Gelvatol or Mowiol Protocol]

Silver Staining Protocol - http://www.lamondlab.com/pdf/silver_staining.pdf

Category: Proteomic Protocols: Silver Staining Protocols

Silver Staining Protocol. Compatible with mass spectrometry, excellent signal-to-noise ratio. Lammond Lab. - [Read Silver Staining Protocol]

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DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.