comfortable Protocols

Category: DNA Protocols: DNA Extraction Protocols: Extracting Ancient DNA

DNA Isolation from museum specimens: 1) what's the best tissue to sample; 2) what's the best way to get DNA out of that tissue? 1) the easiest well-dried tissue to get, and 2) the easiest extraction methods you are comfortable doing. Travis Glenn. - [Read Getting DNA from Old Dead Stuff]

Category: Primary Cell Isolation and Culture Protocols

There are several manual methods that can be used to perform tissue microdissection. Techniques using hand-held tools as well as mechanical micromanipulator-based approaches have been described. However, speed and precision are the most important parameters and any method that achieves these is adequate. Investigators should also expect to invest time initially by practicing on 10 to 20 cases to begin to feel comfortable with the technique. - [Read Manual Microdissection]