columbia Protocols

Search results for: columbia

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Blocking of Unwanted Non-Specific Staining Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/NonSpecificBlocking.html

Category: Immunohistochemistry Protocols: Blocking Protocols

Protocol for blocking of unwanted non-specific staining. Includes: Blocking of endogenous enzymes; Blocking of endogenous fluorochromes; Blocking endogenous biotin; Blocking of endogenous Fc blocking; Blocking of crossreactive antigens in the tissue. - [Read Blocking of Unwanted Non-Specific Staining Protocol]

Blocking of unwanted non-specific staining in Immunofluorescence. - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/NonSpecificBlocking.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Blocking of unwanted non-specific staining in Immunofluorescence. Blocking of endogenous enzymes, Blocking of endogenous fluorochromes, Blocking endogenous biotin , Blocking of endogenous Fc blocking, Blocking of crossreactive antigens in the tissue. Cattoretti. Columbia University - [Read Blocking of unwanted non-specific staining in Immunofluorescence.]

Blocking Solutions Protocols - http://icg.cpmc.columbia.edu/cattoretti/Protocol/main_page_reagents.html#Blocking%20solutions

Category: Immunohistochemistry Protocols: Blocking Protocols

Protocols for blocking solutions. Includes: Endogenous peroxidase blocking solution (H2O2-Azide); Non-specific antibody background blocking solution: swine serum; Non-specific antibody background blocking solution: MILK; Enzymatic antigen retrieval solution; Unconjugated, biotin- and fluorochrome-conjugated antibody (primary) solutions; Unconjugated, biotin- and fluorochrome-conjugated antibody (secondary) solutions; Enzyme-conjugated secondary antibody solutions.; etc.. - [Read Blocking Solutions Protocols]

Collection of Mouse Peritoneal Cells Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/mousepathology/CollectPerC.html

Category: Mouse Protocols

Protocol for the collection of mouse peritoneal cells. - [Read Collection of Mouse Peritoneal Cells Protocol]

Dissociation of Mouse Spleen and Hemopoietic Tissue Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/mousepathology/DissociationSpleen.html

Category: Mouse Protocols

Protocol for the dissociation of mouse spleen and hemopoietic tissue. - [Read Dissociation of Mouse Spleen and Hemopoietic Tissue Protocol]

Double AP Staining Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/nbtbcipstaining.html#Double%20AP%20staining

Category: Histology Protocols: Histology Staining Procedures

Protocol for double AP staining. - [Read Double AP Staining Protocol]

Double immunofluorescence staining for BCL-6 - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/DoubleIFstain.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Double immunofluorescence staining for BCL-6. unfixed or acetone-fixed specimens. dewaxed, antigen retrieved slides - [Read Double immunofluorescence staining for BCL-6]

Double Immunofluorescence Staining for BCL-6 Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/DoubleIFstain.html

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Protocol for double immunofluorescence staining for BCL-6. Any type of tissue is suitable for this technique, as long as the antigenicity for your antigen(s) is preserved. Includes: unfixed or acetone-fixed specimens; dewaxed, antigen retrieved slides. - [Read Double Immunofluorescence Staining for BCL-6 Protocol]

Immunohistochemistry on Fixed, Paraffin-Embedded Sections Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/immunohistochemistryMWO.html

Category: Immunohistochemistry Protocols

Protocol for immunohistochemistry on fixed, paraffin-embedded sections. This method is widely used and applies to the detection of the overwhelming majority of antigens, with few exceptions for which enzymatic retrieval is required. The method uses a strong chelating agent, EDTA. Includes: Double indirect AP; AP Developing solution; Indirect immunohistochemistry with avidin-biotin and HRP; HRP Developing solution. - [Read Immunohistochemistry on Fixed, Paraffin-Embedded Sections Protocol]

Immunohistochemistry with AP-Conjugated (NBT/BCIP) Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/NBTBCIPstaining.html

Category: Immunohistochemistry Protocols

Protocol for immunohistochemistry with AP-Conjugated (NBT/BCIP). Protocol extensively blocks slides, further diluting the primary antibody, lengthening the incubation and washing time, using a simple AP-conjugated secondary at high dilution and use a slow long development with the most powerful IHC development, NBT/BCIP. Includes: Single AP stainiing and Double AP staining. - [Read Immunohistochemistry with AP-Conjugated (NBT/BCIP) Protocol]

In Situ Hybridization for Epstein Barr Virus-Encoded RNA (EBER) Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/EBER-ISH4doublestain.html

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for in situ hybridization for Epstein Barr virus-encoded RNA (EBER). Includes: Glassware decontamination; Double staining for EBER and a second antibody . - [Read In Situ Hybridization for Epstein Barr Virus-Encoded RNA (EBER) Protocol]

Preparation of cytospin from single cell suspension - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/CytospinPreparation.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Preparation of cytospin from single cell suspension. Columbia University. Cattoretti Lab. - [Read Preparation of cytospin from single cell suspension]

Preparing a cell block from cell lines / cell suspensions - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/Cell_block.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Preparing a cell block from cell lines / cell suspensions. Cattoretti, Colombia University. - [Read Preparing a cell block from cell lines / cell suspensions]

Preparing a Cell Block from Cell Lines Cell Suspensions Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/Cell_block.html

Category: Immunohistochemistry Protocols: Blocking Protocols

Protocol for preparing a cell block from cell lines / cell suspensions. Includes: Live cell preparation; Fixed cell preparation. - [Read Preparing a Cell Block from Cell Lines Cell Suspensions Protocol]

Protein extraction from whole tissues for isoelectric focusing - http://proteomics.missouri.edu/protocols/proteinExtraction.html

Category: Protein Protocols: Protein Extraction From Tissue

Protein extraction from whole tissues for isoelectric focusing. University of Missouri - Columbia Proteomics Center. SDS extraction followed by acetone precipitation. Also has Phenol extraction followed by methanolic ammonium acetate precipitation. - [Read Protein extraction from whole tissues for isoelectric focusing]

Single AP staining Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/nbtbcipstaining.html#PROCEDURE

Category: Histology Protocols: Histology Staining Procedures

Protocol for single AP staining. - [Read Single AP staining Protocol]